Mouse uPA Receptor/U-PAR ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse uPA Receptor/U-PAR with a sensitivity of 6.354 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Urine, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 39.063 - 2500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 6.354 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA.
Alternative names
CD87, Urokinase plasminogen activator surface receptor, U-PAR, uPAR, Plaur
What's included?
Recommended products
Mouse uPA Receptor/U-PAR ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse uPA Receptor/U-PAR with a sensitivity of 6.354 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Urine, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 39.063 - 2500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 6.354 pg/mL
- General recovery
- 102%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 2.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 102 | Range 102 - 104 % |
Sample type EDTA Plasma | Average % = 90 | Range 84 - 96 % |
Sample type Citrate plasma | Average % = 95 | Range 91 - 98 % |
Sample type Heparin Plasma | Average % = 106 | Range 99 - 110 % |
Sample type Urine | Average % = 99 | Range 96 - 103 % |
Sample type Cell culture supernatant | Average % = 108 | Range 103 - 113 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse uPA Receptor/U-PAR SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of uPA Receptor/U-PAR protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Urine, Cell culture supernatant. Quantitate Mouse uPA Receptor/U-PAR with 6.354 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sandwich ELISA - Mouse uPA Receptor/U-PAR ELISA Kit (ab315782)
Example of mouse uPA Receptor/U-PAR standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse uPA Receptor/U-PAR ELISA Kit (ab315782)
Interpolated concentration of native uPA Receptor/U-PAR was measured in duplicate at different sample concentrations. Undiluted samples are as follows: mouse serum 15%, plasma – citrate 50%, plasma – EDTA 50%, and plasma – heparin 50%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in of Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Mouse uPA Receptor/U-PAR ELISA Kit (ab315782)
Interpolated concentration of native uPA Receptor/U-PAR was measured in duplicate at different sample concentrations. Undiluted samples are as follows: mouse urine 3% and RAW264.7 cell culture supernatant 12.5%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in of Sample Diluent NS + 1X Enhancer.
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Product protocols
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