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AB245726

Mouse Uromodulin ELISA Kit

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(3 Publications)

Mouse Uromodulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Uromodulin with a sensitivity of 212 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Uromodulin, Tamm-Horsfall urinary glycoprotein, THP, Umod

11 Images
ELISA - Mouse Uromodulin ELISA Kit (AB245726)
  • ELISA

Supplier Data

ELISA - Mouse Uromodulin ELISA Kit (AB245726)
Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)

Interpolated concentrations of spiked Uromodulin in cell culture media. The concentrations of Uromodulin were measured in duplicates, interpolated from the Uromodulin standard curves and corrected for sample dilution. Undiluted samples are as follows : cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)

Interpolated concentrations of native Uromodulin in mouse urine. The concentrations of Uromodulin were measured in duplicates, interpolated from the Uromodulin standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uromodulin concentration was determined to be 12,867 ng/mL in urine.

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)

Interpolated concentrations of native Uromodulin in mouse serum, plasma samples. The concentrations of Uromodulin were measured in duplicates, interpolated from the Uromodulin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 6.25%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uromodulin concentration was determined to be 28,874 pg/mL in serum, 21.506 pg/mL in plasma (citrate), 27,219 pg/mL in plasma (EDTA), and 39,374 pg/mL in plasma (heparin).

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)

Example of mouse Uromodulin standard curve in 1X Cell Extraction Buffer PTR. The Uromodulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)

Interpolated concentrations of native Uromodulin in mouse kidney tissue extract based on a 100 μg/mL extract load. The concentrations of Uromodulin were measured in duplicate and interpolated from the Uromodulin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uromodulin concentration was determined to be 41,624 pg/mL in kidney tissue extract.

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)

Example of mouse Uromodulin standard curve in Sample Diluent NS. The Uromodulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)
  • sELISA

Lab

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)

Interpolated concentration of native Uromodulin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 400 mouse urine.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)
  • sELISA

Lab

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)

Interpolated concentration of native Uromodulin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50 µg/mL mouse kidney tissue extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)
  • sELISA

Lab

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)

Example of mouse Uromodulin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)
  • sELISA

Lab

Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726)

Example of mouse Uromodulin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Tissue Extracts, Urine

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 212 pg/mL

Range

625 - 40000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Uromodulin ELISA Kit ab245726 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Uromodulin in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts, urine. This SimpleStep sensitivity is 212 pg/mL.

How the assay works

Mouse Uromodulin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Uromodulin ELISA Kit ab245726 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Mouse Uromodulin ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-UMOD antibody [EPR22133-150] - BSA and Azide free (Capture) ab259582
- Anti-UMOD antibody [EPR22133-142] - BSA and Azide free (Detector) ab259582

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]
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Recovery

[ { "sample": "Serum", "range": "80 - 92 %", "average": "= 87" }, { "sample": "EDTA Plasma", "range": "87 - 91 %", "average": "= 89" }, { "sample": "Urine", "range": "112 - 121 %", "average": "= 117" }, { "sample": "Cell culture media", "range": "99 - 100 %", "average": "= 99" }, { "sample": "Tissue Extracts", "range": "92 - 96 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "82 - 87 %", "average": "= 84" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UMOD also known as uromodulin or Tamm-Horsfall protein is a glycoprotein with an approximate mass of 85 to 90 kDa. This protein is mostly expressed in the thick ascending limb of the loop of Henle in the kidney. Uromodulin is secreted into urine and represents the most abundant protein in normal urine. Its function is to contribute to the water impermeability of the thick ascending limb impact on concentration of urine and provide a barrier to bacteria adhering in the urinary tract.
Biological function summary

Uromodulin plays a role in preventing urinary tract infections by inhibiting the adhesion of bacteria through its glycoprotein structure. It forms a gel-like layer in the urinary tract that traps pathogens. Uromodulin also participates in immune regulation serving as a modulator by interacting with immune cells. Additionally it influences renal handling of ions which impacts blood pressure regulation.

Pathways

Uromodulin is involved in pathways associated with water and salt balance in the kidneys. This protein interacts with NKCC2 the sodium-potassium-chloride cotransporter to mediate ion transport in the thick ascending limb of the loop of Henle. Uromodulin also connects to the renin-angiotensin system helping to regulate blood pressure by influencing sodium reabsorption and vascular functions.

Uromodulin is linked to disorders such as familial juvenile hyperuricemic nephropathy and medullary cystic kidney disease type 2. These conditions stem from mutations in the UMOD gene leading to abnormal protein folding. It's noted that UMOD mutations can alter interactions with proteins like NKCC2 impacting ion transport and contributing to the pathophysiology of these kidney disorders.
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Product protocols

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Target data

Uromodulin. Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability. May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelia.. Uromodulin, secreted form. In the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes and inhibits formation of liquid containing supersaturated salts and subsequent formation of salt crystals (PubMed : 14871399, PubMed : 15327412). Protects against urinary tract infections by binding to type 1 fimbriated E.coli (PubMed : 11134021). Binds to the bacterial adhesin fimH which mediates the stable formation of bacterial aggregates, prevents the binding of E.coli to uroplakins UPK1A and UPK1B which act as urothelial receptors for type I fimbriae, and allows for pathogen clearance through micturation (By similarity). Also promotes aggregation of other bacteria including K.pneumoniae, P.aeruginosa and S.mitis and so may also protect against other uropathogens (By similarity).
See full target information Umod
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Acta physiologica (Oxford, England) 233:e13705 PubMed34114742

2021

A five amino acids deletion in NKCC2 of C57BL/6 mice affects analysis of NKCC2 phosphorylation but does not impact kidney function.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Moser,Yuya Sugano,Agnieszka Wengi,Viktoria Fisi,Lena Lindtoft Rosenbaek,Marta Mariniello,Dominique Loffing-Cueni,James A McCormick,Robert A Fenton,Johannes Loffing

The American journal of pathology 190:2436-2452 PubMed32926855

2020

A Uromodulin Mutation Drives Autoimmunity and Kidney Mononuclear Phagocyte Endoplasmic Reticulum Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Plotkin,Charles A O'Brien,Joseph Goellner,Joshua Williams,Weleetka Carter,Shree Sharma,Annjanette Stone

Scientific reports 9:19517 PubMed31863061

2019

The Urinary Excretion of Uromodulin is Regulated by the Potassium Channel ROMK.

Applications

Unspecified application

Species

Unspecified reactive species

Guglielmo Schiano,Bob Glaudemans,Eric Olinger,Nadine Goelz,Michael Müller,Dominique Loffing-Cueni,Georges Deschenes,Johannes Loffing,Olivier Devuyst
View all publications
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