Mouse Uromodulin ELISA Kit, 90-min ELISA (ab245726)

Mouse Uromodulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Uromodulin with a sensitivity of 212 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

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Sandwich ELISA - Mouse Uromodulin ELISA Kit (AB245726), expandable thumbnail

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Key facts

Detection method: Colorimetric
Sample types: Cell culture media, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Tissue Extracts, Urine
Assay type: Sandwich (quantitative)
Reactive species: Mouse
Range: 625 - 40000 pg/mL
Assay time: 1h 30m
Sensitivity: = 212 pg/mL

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Target data

See full target information Umod

Function

Uromodulin. Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability. May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelia. Uromodulin, secreted form. In the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes and inhibits formation of liquid containing supersaturated salts and subsequent formation of salt crystals (PubMed:14871399, PubMed:15327412). Protects against urinary tract infections by binding to type 1 fimbriated E.coli (PubMed:11134021). Binds to the bacterial adhesin fimH which mediates the stable formation of bacterial aggregates, prevents the binding of E.coli to uroplakins UPK1A and UPK1B which act as urothelial receptors for type I fimbriae, and allows for pathogen clearance through micturation (By similarity). Also promotes aggregation of other bacteria including K.pneumoniae, P.aeruginosa and S.mitis and so may also protect against other uropathogens (By similarity).

Alternative names

Uromodulin, Tamm-Horsfall urinary glycoprotein, THP, Umod

What's included?

1 x 384 Tests

Components

10X Mouse Uromodulin Capture Antibody

1 x 600 µL

10X Mouse Uromodulin Detector Antibody

1 x 600 µL

10X Wash Buffer PT (ab206977)

1 x 20 mL

384 well CaptSure™ microplates

1 x 1 Unit

5X Cell Extraction Buffer PTR (ab193970)

1 x 50 mL

Antibody Diluent 4BR

1 x 6 mL

Mouse Uromodulin Lyophilized Recombinant Protein

2 x 1 Vial

Plate Seals

1 x 1 Unit

Sample Diluent NS (ab193972)

2 x 50 mL

SimpleStep Pre-Coated 96-Well Microplate (ab206978)

1 x 1 Unit

Stop Solution

2 x 12 mL

TMB Development Solution

2 x 12 mL

Key facts

Detection method: Colorimetric
Sample types: Cell culture media, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Tissue Extracts, Urine
Assay type: Sandwich (quantitative)
Reactive species: Mouse
Range: 625 - 40000 pg/mL
Assay time: 1h 30m
Assay Platform: Pre-coated microplate (12 x 8 well strips)
Sensitivity: = 212 pg/mL

Precision

Intra assay

Sample	n	mean	SD	C.V.
Sample Serum	n 8	mean -	SD -	C.V. 4

Inter assay

Sample	n	mean	SD	C.V.
Sample Serum	n 3	mean -	SD -	C.V. 1.2

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Serum	Average % = 87	Range 80 - 92 %
Sample type EDTA Plasma	Average % = 89	Range 87 - 91 %
Sample type Urine	Average % = 117	Range 112 - 121 %
Sample type Cell culture media	Average % = 99	Range 99 - 100 %
Sample type Tissue Extracts	Average % = 94	Range 92 - 96 %
Sample type Citrate plasma	Average % = 84	Range 82 - 87 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Mouse Uromodulin ELISA Kit (ab245726) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Uromodulin protein in cit plasma, edta plasma, hep plasma, serum, tissue extracts, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Uromodulin with 212 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

UMOD also known as uromodulin or Tamm-Horsfall protein is a glycoprotein with an approximate mass of 85 to 90 kDa. This protein is mostly expressed in the thick ascending limb of the loop of Henle in the kidney. Uromodulin is secreted into urine and represents the most abundant protein in normal urine. Its function is to contribute to the water impermeability of the thick ascending limb impact on concentration of urine and provide a barrier to bacteria adhering in the urinary tract.

Biological function summary

Uromodulin plays a role in preventing urinary tract infections by inhibiting the adhesion of bacteria through its glycoprotein structure. It forms a gel-like layer in the urinary tract that traps pathogens. Uromodulin also participates in immune regulation serving as a modulator by interacting with immune cells. Additionally it influences renal handling of ions which impacts blood pressure regulation.

Pathways

Uromodulin is involved in pathways associated with water and salt balance in the kidneys. This protein interacts with NKCC2 the sodium-potassium-chloride cotransporter to mediate ion transport in the thick ascending limb of the loop of Henle. Uromodulin also connects to the renin-angiotensin system helping to regulate blood pressure by influencing sodium reabsorption and vascular functions.

Associated diseases and disorders

Uromodulin is linked to disorders such as familial juvenile hyperuricemic nephropathy and medullary cystic kidney disease type 2. These conditions stem from mutations in the UMOD gene leading to abnormal protein folding. It's noted that UMOD mutations can alter interactions with proteins like NKCC2 impacting ion transport and contributing to the pathophysiology of these kidney disorders.

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10 product images

Sandwich ELISA - Mouse Uromodulin ELISA Kit (ab245726)
Example of mouse Uromodulin standard curve in Sample Diluent NS. The Uromodulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Uromodulin ELISA Kit (ab245726)
Example of mouse Uromodulin standard curve in 1X Cell Extraction Buffer PTR. The Uromodulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Uromodulin ELISA Kit (ab245726)
Interpolated concentrations of native Uromodulin in mouse serum, plasma samples. The concentrations of Uromodulin were measured in duplicates, interpolated from the Uromodulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 6.25%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uromodulin concentration was determined to be 28,874 pg/mL in serum, 21.506 pg/mL in plasma (citrate), 27,219 pg/mL in plasma (EDTA), and 39,374 pg/mL in plasma (heparin).
Sandwich ELISA - Mouse Uromodulin ELISA Kit (ab245726)
Interpolated concentrations of native Uromodulin in mouse urine. The concentrations of Uromodulin were measured in duplicates, interpolated from the Uromodulin standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 1:400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uromodulin concentration was determined to be 12,867 ng/mL in urine.
Sandwich ELISA - Mouse Uromodulin ELISA Kit (ab245726)
Interpolated concentrations of spiked Uromodulin in cell culture media. The concentrations of Uromodulin were measured in duplicates, interpolated from the Uromodulin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Mouse Uromodulin ELISA Kit (ab245726)
Interpolated concentrations of native Uromodulin in mouse kidney tissue extract based on a 100 μg/mL extract load. The concentrations of Uromodulin were measured in duplicate and interpolated from the Uromodulin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uromodulin concentration was determined to be 41,624 pg/mL in kidney tissue extract.
Sandwich ELISA - Mouse Uromodulin ELISA Kit (ab245726)
Example of mouse Uromodulin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Uromodulin ELISA Kit (ab245726)
Example of mouse Uromodulin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Uromodulin ELISA Kit (ab245726)
Interpolated concentration of native Uromodulin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50 µg/mL mouse kidney tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Mouse Uromodulin ELISA Kit (ab245726)
Interpolated concentration of native Uromodulin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:400 mouse urine. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.