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AB325735

Mouse Uromodulin ELISA Kit, Chemiluminescent

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Mouse Uromodulin ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse Uromodulin with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.05 - 120 ng/ml with a sensitivity of 0.02ng/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

Uromodulin, Tamm-Horsfall urinary glycoprotein, THP, Umod

7 Images
Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)
  • sELISA

Lab

Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)
  • sELISA

Lab

Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)

Dynamic range comparison between the Chemiluminescent Mouse Uromodulin SimpleStep ELISA, colorimetric Mouse Uromodulin SimpleStep ELISA (ab245726), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)
  • sELISA

Lab

Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)

Interpolated concentration of native Uromodulin was measured in duplicate at different sample concentrations. Undiluted samples are 25% serum, plasma (citrate), plasma (EDTA) and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)
  • sELISA

Lab

Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)

Standard curve comparison between the Chemiluminescent Mouse Uromodulin SimpleStep ELISA and colorimetric Mouse Uromodulin SimpleStep ELISA (ab245726) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)

Interpolated concentrations of native Uromodulin in mouse kidney tissue extract based on a 100 µg/mL extract load. The concentrations of Uromodulin were measured in duplicate and interpolated from the Uromodulin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Uromodulin concentration was determined to be 41624 pg/mL in kidney tissue extract.

Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)

Interpolated concentrations of native Uromodulin in mouse urine. The concentrations of Uromodulin were measured in duplicates interpolated from the Uromodulin standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 400. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Uromodulin concentration was determined to be 12867 ng/mL in urine.

Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)

Interpolated concentrations of spiked Uromodulin in cell culture media. The concentrations of Uromodulin were measured in duplicates interpolated from the Uromodulin standard curves and corrected for sample dilution. Undiluted samples are as follows : cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2).

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Urine, Cell culture media, Tissue Extracts, Citrate plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.02 ng/mL

Range

0.05 - 120 ng/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Mouse Uromodulin ELISA Kit, Chemiluminescent (ab325735 ) is a rapid single-wash sandwich 55-min ELISA to measure Mouse Uromodulin in Serum, EDTA Plasma, Urine, Cell culture media, Tissue Extracts, Citrate plasma with a sensitivity of 0.02 ng/mL.

How the assay works

Mouse Uromodulin ELISA Kit, Chemiluminescent (ab325735 ) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Uromodulin (ab325735 ) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Uromodulin antibody [EPR22133-150] - BSA and Aside free (Capture) ab259581
- Anti-Uromodulin antibody [EPR22133-142] - BSA and Aside free (Detector) ab259582
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.2" } ]
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Recovery

[ { "sample": "Serum", "range": "80 - 92 %", "average": "= 87" }, { "sample": "EDTA Plasma", "range": "87 - 91 %", "average": "= 89" }, { "sample": "Urine", "range": "112 - 121 %", "average": "= 117" }, { "sample": "Cell culture media", "range": "99 - 100 %", "average": "= 99" }, { "sample": "Tissue Extracts", "range": "92 - 96 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "82 - 87 %", "average": "= 84" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Uromodulin. Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability. May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelia.. Uromodulin, secreted form. In the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes and inhibits formation of liquid containing supersaturated salts and subsequent formation of salt crystals (PubMed : 14871399, PubMed : 15327412). Protects against urinary tract infections by binding to type 1 fimbriated E.coli (PubMed : 11134021). Binds to the bacterial adhesin fimH which mediates the stable formation of bacterial aggregates, prevents the binding of E.coli to uroplakins UPK1A and UPK1B which act as urothelial receptors for type I fimbriae, and allows for pathogen clearance through micturation (By similarity). Also promotes aggregation of other bacteria including K.pneumoniae, P.aeruginosa and S.mitis and so may also protect against other uropathogens (By similarity).
See full target information Umod
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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