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AB100750

Mouse VCAM1 ELISA Kit

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(5 Publications)

Mouse VCAM1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse VCAM1 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

CD106, Vcam-1, Vcam1, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1

2 Images
Sandwich ELISA - Mouse VCAM1 ELISA Kit (AB100750)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse VCAM1 ELISA Kit (AB100750)

Representative Standard Curve using ab100750

Sandwich ELISA - Mouse VCAM1 ELISA Kit (AB100750)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse VCAM1 ELISA Kit (AB100750)

Representative Standard Curve using ab100750

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 1.5 pg/mL

Range

2.05 - 500 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's VCAM1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse VCAM1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Mouse VCAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and VCAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse VCAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VCAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 107 %", "average": "= 97.36" }, { "sample": "Serum", "range": "82 - 105 %", "average": "= 96.43" }, { "sample": "Plasma", "range": "81 - 104 %", "average": "= 94.64" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100750-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100750-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100750-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse VCAM1", "number":"AB100750-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse VCAM1 Standard (lyophilized)", "number":"AB100750-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"120X HRP-Streptavidin Concentrate", "number":"AB100750-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100750-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"VCAM1 Microplate (12 strips x 8 wells)", "number":"AB100750-CMP01", "productcode":"" }, { "size":"2 x 30 mL", "name":"Assay Diluent A", "number":"AB100750-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100750-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.
Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.
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Product protocols

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Target data

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation.
See full target information Vcam1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular biosciences 7:604042 PubMed33777999

2021

Soluble Epoxide Hydrolase Inhibition Prevents Experimental Type 4 Cardiorenal Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Mouad Hamzaoui,Clothilde Roche,David Coquerel,Thomas Duflot,Valery Brunel,Paul Mulder,Vincent Richard,Jérémy Bellien,Dominique Guerrot

Alzheimer's research & therapy 11:44 PubMed31084613

2019

ApoA-I deficiency increases cortical amyloid deposition, cerebral amyloid angiopathy, cortical and hippocampal astrogliosis, and amyloid-associated astrocyte reactivity in APP/PS1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Emily B Button,Guilaine K Boyce,Anna Wilkinson,Sophie Stukas,Arooj Hayat,Jianjia Fan,Brennan J Wadsworth,Jerome Robert,Kris M Martens,Cheryl L Wellington

Molecular medicine reports 18:4486-4498 PubMed30221694

2018

Role of the Rho/ROCK signaling pathway in the protective effects of fasudil against acute lung injury in septic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Xiaofeng Wang,Wei Liu,Lichun Zhang

American journal of physiology. Regulatory, integr 313:R560-R571 PubMed28835451

2017

Obesity-induced vascular inflammation involves elevated arginase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Yao,Anil Bhatta,Zhimin Xu,Jijun Chen,Haroldo A Toque,Yongjun Chen,Yimin Xu,Zsolt Bagi,Rudolf Lucas,Yuqing Huo,Ruth B Caldwell,R William Caldwell

The Journal of nutritional biochemistry 44:52-59 PubMed28399420

2017

Dietary rose hip exerts antiatherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice.

Applications

FuncS

Species

Mouse

Michele Cavalera,Ulrika Axling,Catarina Rippe,Karl Swärd,Cecilia Holm
View all publications
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