Mouse VCAM1 ELISA Kit (CD106) is a single-wash 90-min Simplestep used to quantify Mouse VCAM1 (CD106) with a sensitivity of 5.59 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
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- Nature communications 14:10622023Molecular imaging of liver inflammation using an anti-VCAM-1 nanobody.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaxime Nachit,Christopher Montemagno,Romain Clerc,Mitra Ahmadi,François Briand,Sandrine Bacot,Nick Devoogdt,Cindy Serdjebi,Catherine Ghezzi,Thierry Sulpice,Alexis Broisat,Isabelle A Leclercq,Pascale PerretPubMed 36828835
- Journal of advanced research 54:29-422023LncRNA NIPA1-SO confers atherosclerotic protection by suppressing the transmembrane protein NIPA1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Jiang,Yu Song,Mei-Xia Ren,Run-Chao He,Xian-Hui Dong,Xue-Heng Li,Zhi-Feng Lu,Shu Li,Jia Wu,Yan-Rou Bei,Fei Liu,Yan Long,Shao-Guo Wu,Xue-Hui Liu,Li-Mei Wu,Hong-Ling Yang,David G McVey,Xiao-Yan Dai,Shu Ye,Yan-Wei HuPubMed 36736696
- Theranostics 12:4564-45802022FGFR blockade boosts T cell infiltration into triple-negative breast cancer by regulating cancer-associated fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYushen Wu,Ziying Yi,Jie Li,Yuxian Wei,Rui Feng,Jiazhou Liu,Jiefeng Huang,Yuru Chen,Xiaoyu Wang,Jiazheng Sun,Xuedong Yin,Yunhai Li,Jingyuan Wan,Li Zhang,Jing Huang,Huimin Du,Xiaoyi Wang,Qin Li,Guosheng Ren,Hongzhong LiPubMed 35832090
- Experimental and therapeutic medicine 23:3032022Sevoflurane preconditioning prevents acute renal injury caused by ischemia-reperfusion in mice via activation of the Nrf2 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen-Xi Wang,Zhen-Ru Zhao,Ying Bai,Ya-Xing Li,Xiao-Ning Gao,Sen Zhang,Yan-Bin SunPubMed 35340877
- Frontiers in nutrition 9:8314852022Effects of Diosmin on Vascular Leakage and Inflammation in a Mouse Model of Venous Obstruction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunjie Zou,Dongping Yuan,Jin Yang,Yun YuPubMed 35273990
- Folia neuropathologica 59:359-3712022USP38 might be a new therapeutic target for glioma via regulation of cancer cell metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Hu,Ke-Ni Wang,Hai-Rui LingHuPubMed 35114776
- Frontiers in cardiovascular medicine 8:7855542022Diosmin Alleviates Venous Injury and Muscle Damage in a Mouse Model of Iliac Vein Stenosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiye Guo,Xiaolong Du,Yihua Zhang,Chunwan Su,Feng Ran,Qiulun LuPubMed 35097005
- Journal of cellular biochemistry 119:4514-45272018Effects of microRNA-330 on vulnerable atherosclerotic plaques formation and vascular endothelial cell proliferation through the WNT signaling pathway in acute coronary syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Ren,Rui Ma,Zhu-Bo Zhang,Yang Li,Ping Lei,Jian-Long MenPubMed 29236323
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 46.9 - 3000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 5.59 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation.
Alternative names
CD106, Vcam-1, Vcam1, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1
What's included?
Recommended products
Mouse VCAM1 ELISA Kit (CD106) is a single-wash 90-min Simplestep used to quantify Mouse VCAM1 (CD106) with a sensitivity of 5.59 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 46.9 - 3000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 5.59 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 8 | mean - | SD - | C.V. 4.3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 5.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 107 | Range 104 - 109 % |
Sample type EDTA Plasma | Average % = 109 | Range 106 - 111 % |
Sample type Heparin Plasma | Average % = 108 | Range 107 - 108 % |
Sample type Citrate plasma | Average % = 111 | Range 109 - 113 % |
Sample type Cell culture media | Average % = 87 | Range 86 - 88 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse VCAM1 ELISA Kit (CD106) (ab201278) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VCAM1 (CD106) protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse VCAM1 (CD106) with 5.59 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
VCAM1 is important in cell-cell recognition, and appears to function in leukocyte-endothelial cell adhesion. It interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.
- Biological function summary
Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.
- Pathways
VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.
- Associated diseases and disorders
Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.
Product promise
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5 product images
Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (ab201278)
Mouse VCAM1 (CD106) standard curve comparison data.
Standard curve comparison between mouse VCAM1 (CD106) SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 9-fold increase in sensitivity.
Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (ab201278)
Example of VCAM1 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (ab201278)
Example of VCAM1 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (ab201278)
Titration of mouse serum, citrate plasma, EDTA plasma, and Heparin plasma within the working range of the assay.
The 1X dilution was 0.2% for each biological. Interpolated data values (mean +/- SD, n = 2) are graphed.
Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (ab201278)
Titration of mouse unstimulated (-) Lung and L929 extracts within the working range of the assay.
The 1X dilution was an extract load of 250 μg/mL. Interpolated data values (mean +/- SD, n = 2) are graphed.
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