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AB206980

Mouse VE Cadherin ELISA Kit

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Mouse VE Cadherin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse VE Cadherin with a sensitivity of 167.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD144, Cadherin-5, Vascular endothelial cadherin, VE-cadherin, Cdh5

2 Images
Sandwich ELISA - Mouse VE Cadherin ELISA Kit (AB206980)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse VE Cadherin ELISA Kit (AB206980)

Linearity of dilution of native mouse VE-Cadherin protein in mouse serum and plasma.

The concentrations of mouse VE-Cadherin were measured in duplicate and interpolated from the mouse VE-Cadherin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD). 1X serum is at a concentration of 0.25%, and 1X plasma (citrate and EDTA) is at a concentration of 0.5%, and 1X plasma (heparin) is at a concentration of 0.25%.

Sandwich ELISA - Mouse VE Cadherin ELISA Kit (AB206980)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse VE Cadherin ELISA Kit (AB206980)

Example of VE-Cadherin standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 167.6 pg/mL

Range

234.37 - 15000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Primary Antibodies

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Anti-VE Cadherin antibody - Intercellular Junction Marker

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse VE Cadherin ELISA Kit (ab206980) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VE Cadherin protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse VE Cadherin with 167.6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

VE-Cadherin (also known as Cadherin 5 or CD144) is a calcium-dependent cell-cell adhesion glycoprotein. VE-Cadherin is a member of the cadherin superfamily, which interact with themselves in connecting cells. VE-Cadherin is expressed in endothelial cells and interacts with KRIT1 to establish and maintain endothelial polarity.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" } ]
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Recovery

[ { "sample": "Serum", "range": "107 - 109 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "94 - 103 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "86 - 100 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "105 - 109 %", "average": "= 106" }, { "sample": "Tissue Culture Media", "range": "101 - 104 %", "average": "= 103" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab206980-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB206980-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB206980-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB206980-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB206980-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB206980-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB206980-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse VE-Cadherin Lyophilized Recombinant Protein", "number":"AB206980-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB206980-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse VE-Cadherin Capture Antibody", "number":"AB206980-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse VE-Cadherin Detector Antibody", "number":"AB206980-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VE Cadherin also known as CD144 or Cadherin-5 is a protein of about 135 kDa involved in cell adhesion. It is a transmembrane protein characterized by its presence at endothelial cell junctions. VE Cadherin belongs to the cadherin superfamily and its expression predominantly occurs in vascular endothelial cells. This protein facilitates the adhesion between adjacent endothelial cells maintaining the integrity of endothelial barriers.
Biological function summary

VE Cadherin performs essential roles in vascular development and permeability. It is a component of adherens junctions and connects with catenins to mediate cell-cell adhesion through calcium-dependent binding. This protein's adhesive function is critical for blood vessel maturation and response to inflammatory stimuli. Altering VE Cadherin expression impacts vascular stability and endothelial cell layering.

Pathways

VE Cadherin interacts with the Wnt and VEGF signaling pathways. It plays a role in the regulation of endothelial cell survival and migration. The interaction with β-catenin is important in the Wnt pathway influencing the transcription of target genes that modulate cell behavior. In the VEGF pathway VE Cadherin's association with protein kinase C highlights its involvement in endothelial permeability and angiogenesis processes.

VE Cadherin dysregulation links to cancer progression and atherosclerosis. Its role in maintaining endothelial barrier integrity implicates it in tumor metastasis where disrupted cell adhesion facilitates cancer cell dissemination. Additionally altered VE Cadherin expression or function associates with atherosclerotic disease where damaged vascular endothelium leads to plaque formation. The connection of VE Cadherin with β-catenin in these conditions highlights its significance in pathological angiogenesis and vascular remodeling.
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Product protocols

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Target data

Cadherins are calcium-dependent cell adhesion proteins (By similarity). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (By similarity). This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (PubMed : 20332120, PubMed : 9220534). It associates with alpha-catenin forming a link to the cytoskeleton (By similarity). Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1 (PubMed : 24806444). Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen (PubMed : 27466317). These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B (By similarity). Positively regulates reorientation of actin stress fibers and endothelial cell reorientation in response to cellular mechantransduction (By similarity). Required for activation of PRKCZ and for localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction (By similarity). Associates with CTNND1/p120-catenin to control CADH5 endocytosis (PubMed : 33972531).
See full target information Cdh5
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websiteProtocolBooklet
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