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AB100751

Mouse VEGF ELISA Kit

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(13 Publications)

Mouse VEGF ELISA Kit is a Sandwich ELISA for the measurement of Mouse VEGF in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Vegf, Vegfa, L-VEGF, Vascular permeability factor, VPF

3 Images
Sandwich ELISA - Mouse VEGF ELISA Kit (AB100751)
  • sELISA

Lab

Sandwich ELISA - Mouse VEGF ELISA Kit (AB100751)

Mouse VEGF measured in various fluids showing quantity (pg) per mL of tested sample

Sandwich ELISA - Mouse VEGF ELISA Kit (AB100751)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse VEGF ELISA Kit (AB100751)

Representative Standard Curve using ab100751

Sandwich ELISA - Mouse VEGF ELISA Kit (AB100751)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse VEGF ELISA Kit (AB100751)

Representative Standard Curve using ab100751

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 2 pg/mL

Range

4.1 - 1000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's VEGF Mouse Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse VEGF in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Mouse VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "83 - 105 %", "average": "= 94.29" }, { "sample": "Serum", "range": "79 - 102 %", "average": "= 87.67" }, { "sample": "Plasma", "range": "81 - 103 %", "average": "= 89.32" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100751-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Recombinant Mouse VEGF Standard (lyophilized)", "number":"AB100751-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"VEGF Microplate (12 strips x 8 wells)", "number":"AB100751-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100751-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100751-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100751-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100751-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse VEGF", "number":"AB100751-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100751-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"160X HRP-Streptavidin Concentrate", "number":"AB100751-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.
Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.
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Product protocols

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Target data

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (PubMed : 35455969).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (PubMed : 26503042). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 16293765). May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity).
See full target information Vegfa
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:112 PubMed38491475

2024

Hypoxia preconditioning of adipose stem cell-derived exosomes loaded in gelatin methacryloyl (GelMA) promote type H angiogenesis and osteoporotic fracture repair.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqun Li,Shuo Fang,Shaohai Wang,Yang Xie,Yan Xia,Panfeng Wang,Zichen Hao,Shuogui Xu,Yuntong Zhang

Folia neuropathologica 60:76-91 PubMed35359148

2022

Inhibition of poly (ADP-ribose) polymerase 1 in neuron cells might enhance the therapeutic effect of leonurine under cholesterol stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hou,Zhigang Huan,Chao Zhang,Zhiyong Chen,Lingmei Ha,Shengxuan Huang

Science advances 6: PubMed33208358

2020

Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqun Li,Lipeng Wang,Biaotong Huang,Yanqiu Gu,Ying Luo,Xin Zhi,Yan Hu,Hao Zhang,Zhengrong Gu,Jin Cui,Liehu Cao,Jiawei Guo,Yajun Wang,Qirong Zhou,Hao Jiang,Chao Fang,Weizong Weng,Xiaofei Chen,Xiao Chen,Jiacan Su

Molecules (Basel, Switzerland) 25: PubMed33171718

2020

The Assessment of the Combined Treatment of 5-ALA Mediated Photodynamic Therapy and Thalidomide on 4T1 Breast Carcinoma and 2H11 Endothelial Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Krzysztof Zduniak,Katarzyna Gdesz-Birula,Marta Woźniak,Kamila Duś-Szachniewicz,Piotr Ziółkowski

Journal of orthopaedic translation 24:12-22 PubMed32518750

2020

Defactinib attenuates osteoarthritis by inhibiting positive feedback loop between H-type vessels and MSCs in subchondral bone.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjun Hu,Hangtian Wu,Ting Xu,Yutian Wang,Hanjun Qin,Zilong Yao,Peisheng Chen,Yongheng Xie,Zhiguo Ji,Kaifan Yang,Yu Chai,Xianrong Zhang,Bin Yu,Zhuang Cui

Stem cell research & therapy 11:86 PubMed32102690

2020

Insulin-like growth factor-II overexpression accelerates parthenogenetic stem cell differentiation into cardiomyocytes and improves cardiac function after acute myocardial infarction in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Sui,Wei Zhang,Tao Tang,Lili Gao,Ting Cao,Hongbo Zhu,Qinghua You,Bo Yu,Tao Yang

Molecular medicine reports 18:4486-4498 PubMed30221694

2018

Role of the Rho/ROCK signaling pathway in the protective effects of fasudil against acute lung injury in septic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Xiaofeng Wang,Wei Liu,Lichun Zhang

Frontiers in cell and developmental biology 4:99 PubMed27709112

2016

Autocrine VEGF Isoforms Differentially Regulate Endothelial Cell Behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Hideki Yamamoto,Helene Rundqvist,Cristina Branco,Randall S Johnson

PloS one 11:e0156198 PubMed27219328

2016

Palmitoylethanolamide Modulates Inflammation-Associated Vascular Endothelial Growth Factor (VEGF) Signaling via the Akt/mTOR Pathway in a Selective Peroxisome Proliferator-Activated Receptor Alpha (PPAR-α)-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanni Sarnelli,Alessandra D'Alessandro,Teresa Iuvone,Elena Capoccia,Stefano Gigli,Marcella Pesce,Luisa Seguella,Nicola Nobile,Giovanni Aprea,Francesco Maione,Giovanni Domenico de Palma,Rosario Cuomo,Luca Steardo,Giuseppe Esposito

American journal of physiology. Gastrointestinal a 307:G813-23 PubMed25169977

2014

Knockout of histidine decarboxylase decreases bile duct ligation-induced biliary hyperplasia via downregulation of the histidine decarboxylase/VEGF axis through PKA-ERK1/2 signaling.

Applications

Unspecified application

Species

Mouse

Allyson Graf,Fanyin Meng,Laura Hargrove,Lindsey Kennedy,Yuyan Han,Taylor Francis,Kyle Hodges,Yoshiyuki Ueno,Quy Nguyen,John F Greene,Heather Francis
View all publications
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