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Mouse VEGF ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse VEGF in Mouse in Plasma, Cell culture supernatant, Serum samples.
Alternative names=Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, Vegfa, Vegf
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
4.1 - 1000 pg/mL
- Sensitivity
< 2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Images
Target data
Function
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity). Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (PubMed:26503042).
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Mouse VEGF ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse VEGF in Mouse in Plasma, Cell culture supernatant, Serum samples.
Alternative names=Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, Vegfa, Vegf
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
4.1 - 1000 pg/mL
- Assay Platform
Microplate
- Sensitivity
< 2 pg/mL
- General recovery
90%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 94.29 | Range 83 - 105 % |
Sample type Serum | Average % = 87.67 | Range 79 - 102 % |
Sample type Plasma | Average % = 89.32 | Range 81 - 103 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Abcam's VEGF Mouse Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse VEGF in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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