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AB100752

Mouse VEGF ELISA Kit

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(2 Publications)

Mouse VEGF ELISA Kit is a Sandwich ELISA for the measurement of Mouse VEGF in Mouse in Cell/Tissue Extracts samples.

View Alternative Names

Vegf, Vegfa, L-VEGF, Vascular permeability factor, VPF

1 Images
Sandwich ELISA - Mouse VEGF ELISA Kit (AB100752)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse VEGF ELISA Kit (AB100752)

Representative Standard Curve using ab100752

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 2 pg/mL

Range

4.1 - 1000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's VEGF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse VEGF in cell lysates and tissue lysates.

This assay employs an antibody specific for Mouse VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Recovery

[ { "sample": "Cell culture extracts", "range": "82 - 103 %", "average": "= 88.62" }, { "sample": "Tissue Extracts", "range": "81 - 102 %", "average": "= 87.87" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100752-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"2X Cell Lysis Buffer", "number":"AB100752-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"VEGF Microplate (12 strips x 8 wells)", "number":"AB100752-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100752-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Sample Diluent Buffer", "number":"AB100752-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100752-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100752-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100752-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse VEGF Standard (lyophilized)", "number":"AB100752-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse VEGF", "number":"AB100752-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"160X HRP-Streptavidin Concentrate", "number":"AB100752-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.
Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.
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Product protocols

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Target data

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (PubMed : 35455969).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (PubMed : 26503042). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 16293765). May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity).
See full target information Vegfa
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 8:1004 PubMed29270129

2017

P311 Deficiency Leads to Attenuated Angiogenesis in Cutaneous Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Song Wang,Xiaorong Zhang,Wei Qian,Daijun Zhou,Xunzhou Yu,Rixing Zhan,Ying Wang,Jun Wu,Weifeng He,Gaoxing Luo

Iranian journal of basic medical sciences 17:231-58 PubMed24904714

2014

The effect of exercise training on the level of tissue IL-6 and vascular endothelial growth factor in breast cancer bearing mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sadegh Amani Shalamzari,Hamid Agha-Alinejad,Shaban Alizadeh,Shirin Shahbazi,Zahra Kashani Khatib,AbdolReza Kazemi,Mohamad Amin Saei,Neda Minayi
View all publications
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Product promise

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