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AB209882

Mouse VEGF ELISA Kit

5

(1 Review)

|

(16 Publications)

Mouse VEGF ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse VEGF with a sensitivity of 0.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations

View Alternative Names

Vegf, Vegfa, L-VEGF, Vascular permeability factor, VPF

6 Images
ELISA - Mouse VEGF ELISA Kit (AB209882)
  • ELISA

Supplier Data

ELISA - Mouse VEGF ELISA Kit (AB209882)
Sandwich ELISA - Mouse VEGF ELISA Kit (AB209882)
  • sELISA

Lab

Sandwich ELISA - Mouse VEGF ELISA Kit (AB209882)

Mouse VEGF standard curve comparison data.

Standard curve comparison between mouse VEGF SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 9-fold increase in sensitivity.

Sandwich ELISA - Mouse VEGF ELISA Kit (AB209882)
  • sELISA

Unknown

Sandwich ELISA - Mouse VEGF ELISA Kit (AB209882)

Interpolated concentrations of native VEGF in mouse lung extract sample based on a 500 μg/mL extract load and in mouse heart extract sample based on a 1,000 μg/mL extract load.

The concentrations of VEGF were measured in duplicate and interpolated from the VEGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGFA concentration was determined to be 842 pg/mL in lung sample and 50 pg/mL in heart sample.

Sandwich ELISA - Mouse VEGF ELISA Kit (AB209882)
  • sELISA

Unknown

Sandwich ELISA - Mouse VEGF ELISA Kit (AB209882)

Example of mouse VEGF standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse VEGF ELISA Kit (AB209882)
  • sELISA

Unknown

Sandwich ELISA - Mouse VEGF ELISA Kit (AB209882)

Interpolated concentrations of native VEGF in Mouse cell culture supernatant samples.

The concentrations of VEGF were measured in duplicates, interpolated from the VEGFA standard curves and corrected for sample dilution. Undiluted samples are as follows : 5 μg/mL LPS-treated P388D1 Day 6 supernatant 7.5%, lung day 5 supernatant 15%, kidney day 5 supernatant 30%, heart day 5 supernatant 30%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF concentration was determined to be 5,249 pg/mL in LPS P388D1 supernatant, 1,785 pg/mL in lung supernatant, 430 pg/mL in kidney supernatant and 30 pg/mL in heart supernatant samples.

Sandwich ELISA - Mouse VEGF ELISA Kit (AB209882)
  • sELISA

Unknown

Sandwich ELISA - Mouse VEGF ELISA Kit (AB209882)

Example of mouse VEGF standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Cell culture supernatant

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 0.3 pg/mL

Range

5.8 - 370 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse VEGF ELISA Kit ab209882 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse VEGF in cell culture extracts, cell culture supernatant, tissue extracts. This SimpleStep sensitivity is 0.3 pg/mL.

How the assay works

Mouse VEGF SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse VEGF ELISA Kit ab209882 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" } ]
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Recovery

[ { "sample": "Cell culture media", "range": "102.9 - 117.1 %", "average": "= 109.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab209882-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB209882-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB209882-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB209882-CMP13", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse VEGF Capture Antibody", "number":"AB209882-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB209882-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse VEGF Lyophilized Recombinant Protein", "number":"AB209882-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB209882-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB209882-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse VEGF Detector Antibody", "number":"AB209882-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB209882-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB209882-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB209882-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.
Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.
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Product protocols

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Target data

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (PubMed : 35455969).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (PubMed : 26503042). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 16293765). May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity).
See full target information Vegfa
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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Integrative cancer therapies 22:15347354231198195 PubMed37694878

2023

Effect of Moxibustion Combined With Cisplatin on Tumor Microenvironment Hypoxia and Vascular Normalization in Lewis Lung Cancer Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ni Mao,Xiaobo Wu,Chaofeng Wang,Huijuan Mao,Jianzi Wei

Advanced biology 7:e2200320 PubMed36988414

2023

VEGFA Promotes Skeletal Muscle Regeneration in Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Yori Endo,Charles D Hwang,Yuteng Zhang,Shayan Olumi,Daniel J Koh,Christina Zhu,Ronald L Neppl,Shailesh Agarwal,Indranil Sinha

Pharmaceutics 15: PubMed36839928

2023

Chitosan Surface-Modified PLGA Nanoparticles Loaded with Cranberry Powder Extract as a Potential Oral Delivery Platform for Targeting Colon Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mona M Mostafa,Maha M Amin,Mohamed Y Zakaria,Mohammed Abdalla Hussein,Marium M Shamaa,Shady M Abd El-Halim

Frontiers in nutrition 9:1060212 PubMed36742002

2023

Surfeit folic acid, protein, and exercise modify oncogenic inflammatory biomarkers and fecal microbiota.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca M Tuska,Sophia M Helm,C Foster Graf,Courtney James,Gabriel Kong,Leah T Stiemsma,David B Green,Susan Edgar Helm

American journal of translational research 15:233-240 PubMed36777829

2023

Concurrent chemoradiotherapy improves prognosis and quality of life of patients with metastatic and recurrent hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shugang Sun,Bin Shen,Ning Wang

Frontiers in cardiovascular medicine 9:981088 PubMed36440014

2022

Intravenous administration of human Muse cells recovers blood flow in a mouse model of hindlimb ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Hori,Tomoya Kitani,Kenji Yanishi,Takaomi Suga,Masaya Kogure,Tetsuro Kusaba,Yoshihiro Kushida,Mari Dezawa,Satoaki Matoba

Nature communications 13:4744 PubMed35961961

2022

Single-atom nanozymes catalytically surpassing naturally occurring enzymes as sustained stitching for brain trauma.

Applications

Unspecified application

Species

Unspecified reactive species

Shaofang Zhang,Yonghui Li,Si Sun,Ling Liu,Xiaoyu Mu,Shuhu Liu,Menglu Jiao,Xinzhu Chen,Ke Chen,Huizhen Ma,Tuo Li,Xiaoyu Liu,Hao Wang,Jianning Zhang,Jiang Yang,Xiao-Dong Zhang

EMBO molecular medicine 14:e14844 PubMed35362189

2022

SARS-CoV-2 spike spurs intestinal inflammation via VEGF production in enterocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Fa-Min Zeng,Ying-Wen Li,Zhao-Hua Deng,Jian-Zhong He,Wei Li,Lijie Wang,Ting Lyu,Zhanyu Li,Chaoming Mei,Meiling Yang,Yingying Dong,Guan-Min Jiang,Xiaofeng Li,Xi Huang,Fei Xiao,Ye Liu,Hong Shan,Huanhuan He

Folia neuropathologica 60:92-104 PubMed35359149

2022

Iduna contributes to the therapeutic effect of DHA in a cell and mouse model of traumatic brain injury via Wnt/MDM2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Shi,Fan Yang,Zhenyu Ge,Zongchun Tang,Kunhu Zhang,Bobo Chen,Hongbin Wang,Jian Hou,Hu Li,Zheng Tang

Brain : a journal of neurology 145:1449-1463 PubMed35048960

2022

VEGF signalling causes stalls in brain capillaries and reduces cerebral blood flow in Alzheimer's mice.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Ali,Kaja Falkenhain,Brendah N Njiru,Muhammad Murtaza-Ali,Nancy E Ruiz-Uribe,Mohammad Haft-Javaherian,Stall Catchers,Nozomi Nishimura,Chris B Schaffer,Oliver Bracko
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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