Mouse VEGF ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse VEGF with a sensitivity of 0.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations
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- Integrative cancer therapies 22:153473542311981952023Effect of Moxibustion Combined With Cisplatin on Tumor Microenvironment Hypoxia and Vascular Normalization in Lewis Lung Cancer Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNi Mao,Xiaobo Wu,Chaofeng Wang,Huijuan Mao,Jianzi WeiPubMed 37694878
- Advanced biology 7:e22003202023VEGFA Promotes Skeletal Muscle Regeneration in Aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYori Endo,Charles D Hwang,Yuteng Zhang,Shayan Olumi,Daniel J Koh,Christina Zhu,Ronald L Neppl,Shailesh Agarwal,Indranil SinhaPubMed 36988414
- Pharmaceutics 15:2023Chitosan Surface-Modified PLGA Nanoparticles Loaded with Cranberry Powder Extract as a Potential Oral Delivery Platform for Targeting Colon Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMona M Mostafa,Maha M Amin,Mohamed Y Zakaria,Mohammed Abdalla Hussein,Marium M Shamaa,Shady M Abd El-HalimPubMed 36839928
- Frontiers in nutrition 9:10602122023Surfeit folic acid, protein, and exercise modify oncogenic inflammatory biomarkers and fecal microbiota.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRebecca M Tuska,Sophia M Helm,C Foster Graf,Courtney James,Gabriel Kong,Leah T Stiemsma,David B Green,Susan Edgar HelmPubMed 36742002
- American journal of translational research 15:233-2402023Concurrent chemoradiotherapy improves prognosis and quality of life of patients with metastatic and recurrent hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShugang Sun,Bin Shen,Ning WangPubMed 36777829
- Frontiers in cardiovascular medicine 9:9810882022Intravenous administration of human Muse cells recovers blood flow in a mouse model of hindlimb ischemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYusuke Hori,Tomoya Kitani,Kenji Yanishi,Takaomi Suga,Masaya Kogure,Tetsuro Kusaba,Yoshihiro Kushida,Mari Dezawa,Satoaki MatobaPubMed 36440014
- Nature communications 13:47442022Single-atom nanozymes catalytically surpassing naturally occurring enzymes as sustained stitching for brain trauma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaofang Zhang,Yonghui Li,Si Sun,Ling Liu,Xiaoyu Mu,Shuhu Liu,Menglu Jiao,Xinzhu Chen,Ke Chen,Huizhen Ma,Tuo Li,Xiaoyu Liu,Hao Wang,Jianning Zhang,Jiang Yang,Xiao-Dong ZhangPubMed 35961961
- EMBO molecular medicine 14:e148442022SARS-CoV-2 spike spurs intestinal inflammation via VEGF production in enterocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFa-Min Zeng,Ying-Wen Li,Zhao-Hua Deng,Jian-Zhong He,Wei Li,Lijie Wang,Ting Lyu,Zhanyu Li,Chaoming Mei,Meiling Yang,Yingying Dong,Guan-Min Jiang,Xiaofeng Li,Xi Huang,Fei Xiao,Ye Liu,Hong Shan,Huanhuan HePubMed 35362189
- Folia neuropathologica 60:92-1042022Iduna contributes to the therapeutic effect of DHA in a cell and mouse model of traumatic brain injury via Wnt/MDM2 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinyu Shi,Fan Yang,Zhenyu Ge,Zongchun Tang,Kunhu Zhang,Bobo Chen,Hongbin Wang,Jian Hou,Hu Li,Zheng TangPubMed 35359149
- Brain : a journal of neurology 145:1449-14632022VEGF signalling causes stalls in brain capillaries and reduces cerebral blood flow in Alzheimer's mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuhammad Ali,Kaja Falkenhain,Brendah N Njiru,Muhammad Murtaza-Ali,Nancy E Ruiz-Uribe,Mohammad Haft-Javaherian,Stall Catchers,Nozomi Nishimura,Chris B Schaffer,Oliver BrackoPubMed 35048960
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 5.8 - 370 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed:35455969). Involved in protecting cells from hypoxia-mediated cell death (PubMed:35455969). VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (PubMed:26503042). Also binds the DEAR/FBXW7-AS1 receptor (PubMed:16293765). May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity).
Alternative names
Vegf, Vegfa, L-VEGF, Vascular permeability factor, VPF
What's included?
Recommended products
Mouse VEGF ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse VEGF with a sensitivity of 0.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 5.8 - 370 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 0.3 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 5 | C.V. 5.6 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 3 | C.V. 7.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture media | Average % = 109.6 | Range 102.9 - 117.1 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse VEGF ELISA Kit (ab209882) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VEGF protein in cell culture extracts, cell culture supernatant, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse VEGF with 0.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
VEGF is a secreted growth factor of PDGF family active in angiogenesis, vasculogenesis and endothelial cell growth both in fetus and adult. Alternative splicing produces isoforms including 120, 164 and 188 amino acid long forms in mouse. VEGF induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. VEGF dimers bind to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, induce their homodimerization and autophosphorylation.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.
- Biological function summary
VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.
- Pathways
Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.
- Associated diseases and disorders
VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.
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5 product images
Sandwich ELISA - Mouse VEGF ELISA Kit (ab209882)
Mouse VEGF standard curve comparison data.
Standard curve comparison between mouse VEGF SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 9-fold increase in sensitivity.
Sandwich ELISA - Mouse VEGF ELISA Kit (ab209882)
Example of mouse VEGF standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse VEGF ELISA Kit (ab209882)
Example of mouse VEGF standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse VEGF ELISA Kit (ab209882)
Interpolated concentrations of native VEGF in Mouse cell culture supernatant samples.
The concentrations of VEGF were measured in duplicates, interpolated from the VEGFA standard curves and corrected for sample dilution. Undiluted samples are as follows: 5 μg/mL LPS-treated P388D1 Day 6 supernatant 7.5%, lung day 5 supernatant 15%, kidney day 5 supernatant 30%, heart day 5 supernatant 30%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF concentration was determined to be 5,249 pg/mL in LPS P388D1 supernatant, 1,785 pg/mL in lung supernatant, 430 pg/mL in kidney supernatant and 30 pg/mL in heart supernatant samples.
Sandwich ELISA - Mouse VEGF ELISA Kit (ab209882)
Interpolated concentrations of native VEGF in mouse lung extract sample based on a 500 μg/mL extract load and in mouse heart extract sample based on a 1,000 μg/mL extract load.
The concentrations of VEGF were measured in duplicate and interpolated from the VEGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGFA concentration was determined to be 842 pg/mL in lung sample and 50 pg/mL in heart sample.
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