AB203367

Mouse VEGF R3 ELISA Kit (FLT4)

Recombinant
SimpleStep
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Mouse VEGF R3 ELISA Kit (FLT4) is a single-wash 90-min Simplestep used to quantify Mouse VEGF R3 (FLT4) with a sensitivity of 17.97 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Flt-4, Vegfr3, Flt4, Vascular endothelial growth factor receptor 3, VEGFR-3, Fms-like tyrosine kinase 4, Tyrosine-protein kinase receptor FLT4, FLT-4

3 Images

Sandwich ELISA - Mouse VEGF R3 ELISA Kit (FLT4) (AB203367)

sELISA

Supplier Data

Sandwich ELISA - Mouse VEGF R3 ELISA Kit (FLT4) (AB203367)

Example of VEGF R3 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse VEGF R3 ELISA Kit (FLT4) (AB203367)

Titration of mouse stimulated (100 ng/mL LPS + 50 ng/mL PMA, 3 days (+)) lung supernatant, and unstimulated (-) lung and liver cell culture supernatants within the working range of the assay.

The 1X dilution is 50.0% for the lung supernatants, and 25.0% for the liver supernatant. Interpolated data values (mean +/- SD, n = 2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse VEGF R3 ELISA Kit (FLT4) (AB203367)

Titration of mouse serum, citrate plasma, heparin plasma, and EDTA plasma within the working range of the assay.

The 1X dilution is 5% for mouse serum, and 2.5% for each of the plasmas. Interpolated data values (mean +/- SD, n = 2) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 17.97 pg/mL

Range

46.9 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse VEGF R3 ELISA Kit (FLT4) (ab203367) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VEGF R3 (FLT4) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse VEGF R3 (FLT4) with 17.97 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

The VEGF receptor family includes three members VEGF R1, VEGF R2, and VEGF R3 (FLT4). These receptors have a characteristic structure that includes 7 Ig-like domains in the extracellular domain and a cytoplasmic tyrosine kinase domain with a long kinase insert region. VEGF R3 is a tyrosine protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD. It plays an essential role in adult lymphangiogenesis, and in the development of the vascular network and cardiovascular system during embryonic development. VEGF R3 promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated VEGF R3 leads to enhanced production of VEGFC and VEGFA, creating a positive feedback loop that enhances FLT4 signaling.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.14" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.59" } ]

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Recovery

[ { "sample": "Serum", "range": "81 - 88 %", "average": "= 85" }, { "sample": "EDTA Plasma", "range": "96 - 104 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "95 - 101 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "101 - 107 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "87 - 88 %", "average": "= 87" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VEGF Receptor 3 also known as FLT-4 is a protein essential for lymphatic development and is expressed mainly in lymphatic endothelial cells. This receptor has a molecular mass of approximately 195 kDa. VEGF Receptor 3 belongs to the tyrosine kinase family and binds with high affinity to its ligands VEGF-C and VEGF-D. These interactions lead to the activation of intracellular signaling cascades that promote cell proliferation migration and survival.

Biological function summary

The VEGF Receptor 3 acts as an important regulator of lymphangiogenesis which is the growth of new lymphatic vessels from pre-existing ones. It is not typically part of larger protein complexes but it works closely with its ligands to facilitate the processes associated with lymph vessel formation and function. Proper activity of VEGF Receptor 3 is necessary for maintaining normal lymphatic vessel remodeling and function during development and in some adult tissues.

Pathways

The receptor is involved in the VEGF signaling pathway which plays an important role in vascular and lymphatic development. This pathway intersects with others like the PI3K/AKT pathway a critical mediator of cell growth and survival. VEGF Receptor 3 interacts with related receptors such as VEGF Receptor 2 in these pathways to regulate complex and essential physiological processes.

VEGF Receptor 3 has been linked to lymphedema which is swelling due to lymphatic system dysfunction and certain cancers where excessive angiogenesis occurs. Mutations or dysregulations in the receptor's signaling can lead to these conditions. In cancer for instance VEGF Receptor 3 along with its associated protein VEGF-C can promote tumor lymphangiogenesis facilitating metastasis. Antagonists like anti-VEGF therapies target these receptors to inhibit tumor progression and manage these disorders.

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Product protocols

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'.

See full target information Flt4

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse VEGF R3 ELISA Kit (FLT4)

Sandwich ELISA - Mouse VEGF R3 ELISA Kit (FLT4) (AB203367)

Sandwich ELISA - Mouse VEGF R3 ELISA Kit (FLT4) (AB203367)

Sandwich ELISA - Mouse VEGF R3 ELISA Kit (FLT4) (AB203367)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Assay Kits

Product promise