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AB289700

Mouse VEGFB ELISA Kit

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Mouse VEGFB ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse VEGFB with a sensitivity of 39.432 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Vrf, Vegfb, Vascular endothelial growth factor B, VEGF-B, VEGF-related factor, VRF

2 Images
Sandwich ELISA - Mouse VEGFB ELISA Kit (AB289700)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse VEGFB ELISA Kit (AB289700)

Interpolated concentrations of spiked mouse VEGFB in cell extract and tissue extract.

Recombinant VEGFB was spiked into the following mouse samples and diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. Undiluted samples are 500 μg/mL B16-F10 cell extract and heart tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Mouse VEGFB ELISA Kit (AB289700)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse VEGFB ELISA Kit (AB289700)

Example of mouse VEGFB standard curve in 1X Cell Extraction Buffer PTR.

Example of mouse VEGFB standard curve. Background-subtracted data values (mean +/- SD) are graphed

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 39.432 pg/mL

Range

171.875 - 11000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse VEGFB ELISA Kit (ab289700) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse VEGFB in cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse VEGFB with 39.432 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12.4" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "102 - 115 %", "average": "= 110" }, { "sample": "Cell Lysate", "range": "92 - 95 %", "average": "= 93" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab289700-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB289700-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB289700-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB289700-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB289700-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB289700-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB289700-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse VEGFB Capture Antibody", "number":"AB289700-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB289700-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse VEGFB Lyophilized Recombinant Protein", "number":"AB289700-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB289700-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse VEGFB Detector Antibody", "number":"AB289700-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VEGFB also known as vascular endothelial growth factor B is a member of the VEGF family. It has a molecular mass of approximately 34-35 kDa. VEGFB interacts with its receptors to promote angiogenesis increasing blood supply to tissues. Expression of VEGFB occurs in the heart skeletal muscle and various endothelial cells. It also shows expression in tissues undergoing high metabolic activities. Besides its angiogenic role VEGFB exhibits participation in metabolic regulation.
Biological function summary

VEGFB supports angiogenesis by promoting endothelial cell growth and migration. It acts as a signal protein not forming part of a larger complex. VEGFB binds to VEGFR-1 (Flt-1) receptor which is involved in transmitting the growth-promoting signals. Its function extends to the regulation of fatty acid uptake into tissues highlighting its role in lipid metabolism and energy utilization.

Pathways

The signalling activities of VEGFB are significant in angiogenesis and metabolic pathways. VEGFB interacts closely with the VEGF-A pathway which is vital for forming new blood vessels. It also shares interactive roles with similar proteins such as VEGF-C and VEGF-D contributing to various angiogenic processes and cellular growth signalling systems.

Understanding VEGFB's role is important in conditions like cardiovascular disease and metabolic syndrome. Altered VEGFB signalling may link to heart diseases due to its regulatory function in maintaining heart vasculature. VEGFB has connections with VEGFR-1 and VEGF-A indicating its potential involvement in endothelial dysfunction and highlighting its significance in therapeutic strategies targeting such diseases.
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Product protocols

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Target data

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. VEGF-B seems to be required for normal heart function in adult but is not required for proper development of the cardiovascular system either during development or for angiogenesis in adults.
See full target information Vegfb
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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