Mouse Von Willebrand Factor A2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Von Willebrand Factor A2 with a sensitivity of 37 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
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- Frontiers in pharmacology 14:13046972023Efficacies of S-nitrosoglutathione (GSNO) and GSNO reductase inhibitor in SARS-CoV-2 spike protein induced acute lung disease in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJudong Kim,Fei Qiao,Avtar K Singh,Jeseong Won,Inderjit SinghPubMed 38143504
- International journal of molecular sciences 24:2023Assaying ADAMTS13 Activity as a Potential Prognostic Biomarker for Sinusoidal Obstruction Syndrome in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasakazu Saeki,Seiichi Munesue,Yuri Higashi,Ai Harashima,Ryohei Takei,Satoshi Takada,Shinichi Nakanuma,Tetsuo Ohta,Shintaro Yagi,Hidehiro Tajima,Yasuhiko YamamotoPubMed 38003518
- International journal of molecular sciences 24:2023Recombinant ADAMTS-13 Improves Survival of Mice Subjected to Endotoxemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Gao,Zhou Zhou,Ruidong Ma,Huaizhu Wu,Trung Nguyen,Li Liu,Jingfei DongPubMed 37511541
- Acta pharmaceutica Sinica. B 11:3791-38052021Dual roles of p62/SQSTM1 in the injury and recovery phases of acetaminophen-induced liver injury in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Qian,Qingyun Bai,Xiao Yang,Jephte Y Akakpo,Lili Ji,Li Yang,Thomas Rülicke,Kurt Zatloukal,Hartmut Jaeschke,Hong-Min Ni,Wen-Xing DingPubMed 35024307
- Medical science monitor : international medical jo 25:4811-48182019Role of Programmed Cell Death 4 (PDCD4)-Mediated Akt Signaling Pathway in Vascular Endothelial Cell Injury Caused by Lower-Extremity Ischemia-Reperfusion in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuanhuan Chen et. al.PubMed 31253757
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 109.38 - 7000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 37 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
Alternative names
von Willebrand factor, vWF, Vwf
What's included?
Recommended products
Mouse Von Willebrand Factor A2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Von Willebrand Factor A2 with a sensitivity of 37 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 109.38 - 7000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 37 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Mouse Plasma | n 5 | C.V. 5.3 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Mouse Plasma | n 3 | C.V. 13.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 83.6 | Range 80.2 - 89.7 % |
Sample type EDTA Plasma | Average % = 94.4 | Range 88.2 - 98.3 % |
Sample type Heparin Plasma | Average % = 82.2 | Range 78.8 - 86.7 % |
Sample type Citrate plasma | Average % = 86.3 | Range 82.7 - 90.3 % |
Sample type serum free media | Average % = 79 | Range 70.9 - 83.8 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse Von Willebrand Factor A2 ELISA Kit (ab208980) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Von Willebrand Factor A2 protein in cit plasma, edta plasma, hep plasma, serum, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Von Willebrand Factor A2 with 37 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
vWF A2 and vWF (von Willebrand Factor) are distinct proteins with non-overlapping amino acid sequences generated by proteolytic cleavage from the same precursor protein during intracellular processing. vWF is important in the maintenance of hemostasis; it is a glycoprotein that circulates in plasma as a series of high molecular weight multimers and mediates the adhesion of platelets to exposed sub-endothelium. vWF is synthesized in endothelial cells and megakaryocytes. vWF A2 is found in plasma and platelets, from which they are released by their activation including thrombin. Both vWF and vWF A2 are deficient in von Willebrand’s disease. .
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3 product images
Sandwich ELISA - Mouse Von Willebrand Factor A2 ELISA Kit (ab208980)
Example of mouse vWF A2 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Von Willebrand Factor A2 ELISA Kit (ab208980)
Interpolated concentrations of native vWF A2 in mouse serum and plasma samples.
The concentrations of vWF A2 were measured in duplicates, interpolated from the vWF A2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 0.75 %, plasma (citrate) 3.5 %, plasma (EDTA) 7.0 %, plasma (heparin) 3.5 %. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean vWF A2 concentration was determined to be 1003 ng/mL in serum, 146 ng/mL in plasma (citrate) 118 ng/mL in plasma (EDTA) and 190 ng/mL in plasma (heparin).
Sandwich ELISA - Mouse Von Willebrand Factor A2 ELISA Kit (ab208980)
Interpolated concentrations of spiked mouse recombinant vWF A2 in serum-free cell culture media samples.
The concentrations of vWF A2 were measured in duplicates, interpolated from the vWF A2 standard curves and corrected for sample dilution. Undiluted samples are as follows: 7,000 pg/mL of vWF A2 in 100% serum-free cell culture media. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean spiked vWF A2 concentration was determined to be 5962 pg/mL in serum-free cell culture media.
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