Mouse Von Willebrand Factor A2 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Mouse Von Willebrand Factor A2 with a sensitivity of 7.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Images
Publications
Filter by
- JCI insight 6:2021NETosis in the pathogenesis of acute lung injury following cutaneous chemical burns.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRanu Surolia,Fu Jun Li,Zheng Wang,Mahendra Kashyap,Ritesh Kumar Srivastava,Amie M Traylor,Pooja Singh,Kevin G Dsouza,Harrison Kim,Jean-Francois Pittet,Jaroslaw W Zmijewski,Anupam Agarwal,Mohammad Athar,Aftab Ahmad,Veena B AntonyPubMed 34027893
Key facts
- Detection method
- Fluorescent
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 13.67 - 7000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 7.1 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
Alternative names
von Willebrand factor, vWF, Vwf
What's included?
Recommended products
Mouse Von Willebrand Factor A2 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Mouse Von Willebrand Factor A2 with a sensitivity of 7.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Key facts
- Detection method
- Fluorescent
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 13.67 - 7000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 7.1 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Plasma | n 5 | C.V. 5.3 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Plasma | n 3 | C.V. 13.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 83.6 | Range 80.2 - 89.7 % |
Sample type EDTA Plasma | Average % = 94.4 | Range 88.2 - 98.3 % |
Sample type Heparin Plasma | Average % = 82.2 | Range 78.8 - 86.7 % |
Sample type Citrate plasma | Average % = 86.3 | Range 82.7 - 90.3 % |
Sample type serum free media | Average % = 79 | Range 70.9 - 83.8 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Von Willebrand Factor A2 in vitro CatchPoint® SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Von Willebrand Factor A2 protein in mouse serum, plasma, and cell culture supernatant samples.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
The CatchPoint® SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
vWF A2 and vWF (von Willebrand Factor) are distinct proteins with non-overlapping amino acid sequences generated by proteolytic cleavage from the same precursor protein during intracellular processing. vWF is important in the maintenance of hemostasis; it is a glycoprotein that circulates in plasma as a series of high molecular weight multimers and mediates the adhesion of platelets to exposed sub-endothelium. vWF is synthesized in endothelial cells and megakaryocytes. vWF A2 is found in plasma and platelets, from which they are released by their activation including thrombin. Both vWF and vWF A2 are deficient in von Willebrand's disease.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sandwich ELISA - Mouse Von Willebrand Factor A2 ELISA Kit, Fluorescent (ab229397)
Example of mouse Von Willebrand Factor A2 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Von Willebrand Factor A2 ELISA Kit, Fluorescent (ab229397)
Interpolated concentrations of native vWF A2 in mouse serum and plasma samples.
The concentrations of vWF A2 were measured in duplicates, interpolated from the vWF A2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 0.75 %, plasma (citrate) 3.5 %, plasma (EDTA) 7.0 %, plasma (heparin) 3.5 %. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean vWF A2 concentration was determined to be 1003 ng/mL in serum, 146 ng/mL in plasma (citrate) 118 ng/mL in plasma (EDTA) and 190 ng/mL in plasma (heparin).
Sandwich ELISA - Mouse Von Willebrand Factor A2 ELISA Kit, Fluorescent (ab229397)
Interpolated concentrations of spiked mouse recombinant vWF A2 in serum-free cell culture media samples.
The concentrations of vWF A2 were measured in duplicates, interpolated from the vWF A2 standard curves and corrected for sample dilution. Undiluted samples are as follows: 7,000 pg/mL of vWF A2 in 100% serum-free cell culture media. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean spiked vWF A2 concentration was determined to be 5962 pg/mL in serum-free cell culture media.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com