AB229397

Mouse Von Willebrand Factor A2 ELISA Kit, Fluorescent

CatchPoint SimpleStep ELISA
Recombinant
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(1 Publication)

Mouse Von Willebrand Factor A2 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Mouse Von Willebrand Factor A2 with a sensitivity of 7.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader

View Alternative Names

von Willebrand factor, vWF, Vwf

3 Images

Sandwich ELISA - Mouse Von Willebrand Factor A2 ELISA Kit, Fluorescent (AB229397)

sELISA

Lab

Sandwich ELISA - Mouse Von Willebrand Factor A2 ELISA Kit, Fluorescent (AB229397)

Example of mouse Von Willebrand Factor A2 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Von Willebrand Factor A2 ELISA Kit, Fluorescent (AB229397)

Interpolated concentrations of spiked mouse recombinant vWF A2 in serum-free cell culture media samples.

The concentrations of vWF A2 were measured in duplicates, interpolated from the vWF A2 standard curves and corrected for sample dilution. Undiluted samples are as follows : 7,000 pg/mL of vWF A2 in 100% serum-free cell culture media. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean spiked vWF A2 concentration was determined to be 5962 pg/mL in serum-free cell culture media.

sELISA

Supplier Data

Sandwich ELISA - Mouse Von Willebrand Factor A2 ELISA Kit, Fluorescent (AB229397)

Interpolated concentrations of native vWF A2 in mouse serum and plasma samples.

The concentrations of vWF A2 were measured in duplicates, interpolated from the vWF A2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 0.75 %, plasma (citrate) 3.5 %, plasma (EDTA) 7.0 %, plasma (heparin) 3.5 %. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean vWF A2 concentration was determined to be 1003 ng/mL in serum, 146 ng/mL in plasma (citrate) 118 ng/mL in plasma (EDTA) and 190 ng/mL in plasma (heparin).

Key facts

Detection method

Fluorescent

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 7.1 pg/mL

Range

13.67 - 7000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Von Willebrand Factor A2 in vitro CatchPoint^® SimpleStep ELISA^® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Von Willebrand Factor A2 protein in mouse serum, plasma, and cell culture supernatant samples.

This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.

The CatchPoint^® SimpleStep ELISA^® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

vWF A2 and vWF (von Willebrand Factor) are distinct proteins with non-overlapping amino acid sequences generated by proteolytic cleavage from the same precursor protein during intracellular processing. vWF is important in the maintenance of hemostasis; it is a glycoprotein that circulates in plasma as a series of high molecular weight multimers and mediates the adhesion of platelets to exposed sub-endothelium. vWF is synthesized in endothelial cells and megakaryocytes. vWF A2 is found in plasma and platelets, from which they are released by their activation including thrombin. Both vWF and vWF A2 are deficient in von Willebrand's disease.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "13.3" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" } ]

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Recovery

[ { "sample": "Serum", "range": "80.2 - 89.7 %", "average": "= 83.6" }, { "sample": "EDTA Plasma", "range": "88.2 - 98.3 %", "average": "= 94.4" }, { "sample": "Heparin Plasma", "range": "78.8 - 86.7 %", "average": "= 82.2" }, { "sample": "Citrate plasma", "range": "82.7 - 90.3 %", "average": "= 86.3" }, { "sample": "serum free media", "range": "70.9 - 83.8 %", "average": "= 79" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab229397-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB229397-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB229397-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB229397-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse vWF A2 Capture Antibody", "number":"AB229397-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse vWF A2 Lyophilized Recombinant Protein", "number":"AB229397-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB229397-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB229397-CMP04", "productcode":"" }, { "size":"1 x 120 µL", "name":"100X Stoplight Red Substrate", "number":"AB229397-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse vWF A2 Detector Antibody", "number":"AB229397-CMP10", "productcode":"" }, { "size":"1 x 50 µL", "name":"500X Hydrogen Peroxide (H2O2, 3%)", "number":"AB229397-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stoplight Red Substrate Buffer", "number":"AB229397-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

See full target information Vwf

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 6: PubMed34027893

2021

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Applications

Unspecified application

Species

Unspecified reactive species

Ranu Surolia,Fu Jun Li,Zheng Wang,Mahendra Kashyap,Ritesh Kumar Srivastava,Amie M Traylor,Pooja Singh,Kevin G Dsouza,Harrison Kim,Jean-Francois Pittet,Jaroslaw W Zmijewski,Anupam Agarwal,Mohammad Athar,Aftab Ahmad,Veena B Antony

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse Von Willebrand Factor A2 ELISA Kit, Fluorescent

Sandwich ELISA - Mouse Von Willebrand Factor A2 ELISA Kit, Fluorescent (AB229397)

Sandwich ELISA - Mouse Von Willebrand Factor A2 ELISA Kit, Fluorescent (AB229397)

Sandwich ELISA - Mouse Von Willebrand Factor A2 ELISA Kit, Fluorescent (AB229397)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

Product promise