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AB314372

Mouse Von Willebrand Factor ELISA Kit

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Mouse Von Willebrand Factor ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Von Willebrand Factor with a sensitivity of 0.045 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

von Willebrand factor, vWF, Vwf

3 Images
ELISA - Mouse Von Willebrand Factor ELISA Kit (AB314372)
  • ELISA

Supplier Data

ELISA - Mouse Von Willebrand Factor ELISA Kit (AB314372)
Sandwich ELISA - Mouse Von Willebrand Factor ELISA Kit (AB314372)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Von Willebrand Factor ELISA Kit (AB314372)

Interpolated concentration of native Von Willebrand Factor was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 1 : 25 serum, 1 : 200 plasma (citrate), 1 : 100 plasma (EDTA), 1 : 40 plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse Von Willebrand Factor ELISA Kit (AB314372)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Von Willebrand Factor ELISA Kit (AB314372)

Example of mouse Von Willebrand Factor standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 0.045 ng/mL

Range

0.359 - 23 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Von Willebrand Factor ELISA Kit ab314372 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Von Willebrand Factor in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 0.045 ng/mL.

How the assay works

Mouse Von Willebrand Factor SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Von Willebrand Factor ELISA Kit ab314372 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
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Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]
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Recovery

[ { "sample": "Serum", "range": "102 - 115 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "102 - 121 %", "average": "= 110" }, { "sample": "EDTA Plasma", "range": "97 - 107 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "92 - 107 %", "average": "= 98" }, { "sample": "Cell culture media", "range": "93 - 102 %", "average": "= 97" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314372-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB314372-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB314372-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB314372-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB314372-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB314372-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Von Willebrand Factor Capture Antibody", "number":"AB314372-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Von Willebrand Factor Lyophilized Recombinant Protein", "number":"AB314372-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Von Willebrand Factor Detector Antibody", "number":"AB314372-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB314372-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB314372-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
See full target information Vwf
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websiteProtocolBooklet
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