JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB290978

Mouse WISP1 ELISA Kit - Extracellular

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse WISP1 ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Mouse WISP1 with a sensitivity of 11.472 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Elm1, Wisp1, Ccn4, CCN family member 4, ELM-1, WNT1-inducible-signaling pathway protein 1, WISP-1

3 Images
Sandwich ELISA - Mouse WISP1 ELISA Kit - Extracellular (AB290978)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse WISP1 ELISA Kit - Extracellular (AB290978)

Interpolated concentration of native WISP1 was measured in duplicate at different sample concentrations. Undiluted samples are 50% C2C12 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse WISP1 ELISA Kit - Extracellular (AB290978)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse WISP1 ELISA Kit - Extracellular (AB290978)

Interpolated concentration of native WISP1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 100% plasma (EDTA) and 40% plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse WISP1 ELISA Kit - Extracellular (AB290978)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse WISP1 ELISA Kit - Extracellular (AB290978)

Example of mouse WISP1 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Cell culture supernatant, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 11.472 pg/mL

Range

28.125 - 1800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB300310

VILIP1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - VILIP1 ELISA Kit (AB300310)

  • 0
  • 0
Assay Kits

AB272476

Mouse CD30-L ELISA Kit (TNFSF8)

Recombinant|SimpleStep

Sandwich ELISA - Mouse CD30-L ELISA Kit (TNFSF8) (AB272476)

  • 0
  • 0
Primary Antibodies

AB285076

Anti-WISP1 antibody [EPR23996-174] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-WISP1 antibody [EPR23996-174] - BSA and Azide free (Detector) (AB285076)

  • 0
  • 0
Assay Kits

AB300311

Human HDAC4 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human HDAC4 ELISA Kit (AB300311)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse WISP1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of WISP1 protein in mouse plasma (heparin), plasma (EDTA), urine, and cell culture supernatant. Quantitate Mouse WISP1 with 11.472 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.2" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "105 - 123 %", "average": "= 114" }, { "sample": "EDTA Plasma", "range": "98 - 119 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "100 - 134 %", "average": "= 114" }, { "sample": "Urine", "range": "75 - 85 %", "average": "= 81" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab290978-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB290978-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB290978-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB290978-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB290978-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB290978-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse WISP1 Capture Antibody", "number":"AB290978-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse WISP1 Lyophilized Recombinant Protein", "number":"AB290978-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB290978-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse WISP1 Detector Antibody", "number":"AB290978-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB290978-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WISP1 also known as Wnt1 Inducible Signaling Pathway Protein 1 or CCN4 is a protein with significant roles in cellular processes. This protein has an approximate mass of 41 kDa. You can find its expression in various tissues including bone cartilage and vascular tissues. WISP1 belongs to the CCN family of proteins which are matricellular proteins that interact with different cell surface molecules and receptor systems to modulate cellular functions.
Biological function summary

WISP1 plays an important role in cellular proliferation differentiation and survival. It functions as a matricellular protein that can form complexes with integrins and other cell surface receptors. This interaction influences cellular responses to environmental signals. WISP1 contributes to extracellular matrix interaction and cellular adhesion. By regulating these processes WISP1 supports tissue development repair and regeneration.

Pathways

The network of interactions influenced by WISP1 impacts several key signaling pathways including the Wnt signaling pathway and the TGF-beta pathway. These pathways are important for regulating cell growth and differentiation. In connection with the Wnt pathway WISP1 interacts with proteins like beta-catenin which plays an important role in signal transduction and gene expression. These interactions help maintain proper cellular function and structural integrity.

WISP1 shows links to cancer and fibrosis. Dysregulation of WISP1 expression and its related pathways can contribute to the development and progression of these conditions. In cancers WISP1 interacts with proteins like beta-catenin and contributes to the regulation of oncogenic processes such as tumor growth and metastasis. In fibrosis WISP1 influences the deposition of extracellular matrix proteins affecting tissue scarring and organ function.
style
left-column

Product protocols

style
left-column

Target data

Downstream regulator in the Wnt/Frizzled-signaling pathway (By similarity). Associated with cell survival. Adheres to skin and melanoma fibroblasts (By similarity). In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan (By similarity). Suppresses tumor growth in vivo.
See full target information Ccn4
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com