Mouse XCL1 ELISA Kit (Lymphotactin)
- Recombinant
- SimpleStep
- What is this?
Be the first to review this product! Submit a review
|
(0 Publication)
Mouse XCL1 ELISA Kit (Lymphotactin) is a single-wash 90-min Simplestep used to quantify Mouse XCL1 (Lymphotactin) with a sensitivity of 6.26 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Lptn, Ltn, Scyc1, Xcl1, Lymphotactin, C motif chemokine 1, Cytokine SCM-1, Lymphotaxin, Small-inducible cytokine C1
- sELISA
Supplier Data
Sandwich ELISA - Mouse XCL1 ELISA Kit (Lymphotactin) (AB269540)
Example of mouse XCL1 standard curve in Sample Diluent NS.
The XCL1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse XCL1 ELISA Kit (Lymphotactin) (AB269540)
Interpolated concentrations of spiked XCL1 in 1640 RPMI cell culture media samples containing 10% fetal bovine serum.
The concentrations of XCL1 were measured in duplicates, interpolated from the XCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows : 1640 RPMI media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Mouse XCL1 ELISA Kit (Lymphotactin) (AB269540)
Interpolated concentrations of native XCL1 in mouse serum and plasma samples.
The concentrations of XCL1 were measured in duplicates, interpolated from the XCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean XCL1 concentration was determined to be 141.47 pg/mL in serum, 118.18 pg/mL in plasma (citrate), 195.80 pg/ml in plasma (heparin), and 162.36 pg/ml in plasma (EDTA).
- sELISA
Supplier Data
Sandwich ELISA - Mouse XCL1 ELISA Kit (Lymphotactin) (AB269540)
Interpolated concentrations of spiked XCL1 in mouse serum and plasma samples.
The concentrations of XCL1 were measured in duplicates, interpolated from the XCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Reactivity data
Product details
Mouse XCL1 ELISA Kit (Lymphotactin) (ab269540) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of XCL1 (Lymphotactin) protein in cell culture media, edta plasma, hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse XCL1 (Lymphotactin) with 6.26 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
XCL1 (Lymphotactin) is a secreted glycosylated protein of C chemokine family. It plays a role in chemotactic activity of lymphocytes, but not monocytes or neutrophils. In the thymus, XCL1 mediates medullary accumulation of dendritic cells and contributes to T cell development which plays a role in establishment of self-tolerance. XCL2 is functionally related XCL1 homolog, these proteins share 98% amino acids sequence identity.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
XCL1 participates in chemotaxis guiding the migration of specific cells to enhance the immune response. Although XCL1 does not form part of a larger protein complex it interacts with its specific receptor XCR1 on target cells. This interaction triggers intracellular signaling pathways contributing to the controlled movement and positioning of immune cells in response to infection or inflammatory signals.
Pathways
XCL1 is significantly involved in the chemokine signaling pathway and plays a part in immune response regulation. This pathway shared with other chemokines like CCL2 and CCL5 orchestrates the precise movement and activity of immune cells. XCL1’s interaction with XCR1 is central to initiating downstream signaling processes which influence immune responses and inflammation.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com