Mouse YKL-40 / CHI3L1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse YKL-40 / CHI3L1 with a sensitivity of 3.17 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Brain, behavior, & immunity - health 26:1005552022Conserved YKL-40 changes in mice and humans after postoperative delirium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer David-Bercholz,Leah Acker,Ana I Caceres,Pau Yen Wu,Saanvi Goenka,Nathan O Franklin,Ramona M Rodriguiz,William C Wetsel,Michael Devinney,Mary Cooter Wright,Henrik Zetterberg,Ting Yang,Miles Berger,Niccolò TerrandoPubMed 36457825
- Biomedicines 10:2022Role of Chitinase-3-like Protein 1 in Cardioprotection and Angiogenesis by Post-Infarction Exercise Training.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhuo Li,Fangnan Wu,Lei Xi,Zhenjun TianPubMed 35625766
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 54.69 - 3500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 3.17 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
Alternative names
Brp39, Chil1, Chi3l1, Chitinase-3-like protein 1, BRP39 protein, Breast regression protein 39, Cartilage glycoprotein 39, CGP-39, GP-39
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Mouse YKL-40 / CHI3L1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse YKL-40 / CHI3L1 with a sensitivity of 3.17 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 54.69 - 3500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 3.17 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 2.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 7.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 109 | Range 106 - 112 % |
Sample type Serum | Average % = 108 | Range 106 - 112 % |
Sample type EDTA Plasma | Average % = 106 | Range 106 - 107 % |
Sample type Heparin Plasma | Average % = 98 | Range 96 - 103 % |
Sample type Citrate plasma | Average % = 98 | Range 96 - 99 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse YKL-40 / CHI3L1 ELISA Kit (ab238262) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of YKL-40 / CHI3L1 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse YKL-40 / CHI3L1 with 3.17 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
YKL-40 also known as chitinase-3-like protein 1 (CHI3L1) is a glycoprotein with a molecular weight of approximately 40 kDa. Scientists often study it for its roles in inflammatory processes. It has expressions in numerous tissues including liver cartilage and the central nervous system. The CHI3L1 protein does not possess chitinase activity unlike other members of the chitinase family yet it interacts with various ligands to influence cellular behavior.
- Biological function summary
YKL-40 plays significant roles in tissue remodeling and inflammation. It does not form part of a larger complex by itself but influences several cellular processes. This protein affects cell proliferation migration and survival making it involved in both normal physiological processes and pathological conditions. CHI3L1 also exhibits a connection to the immune response where it modulates macrophage activity and interacts with other immune cells.
- Pathways
YKL-40 is integral to the inflammatory and extracellular matrix pathways. It associates with proteins such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) influencing signaling cascades that modulate inflammation. Through these interactions CHI3L1 becomes a mediator of connective tissue metabolism and immune reactions in various pathophysiological states.
- Associated diseases and disorders
YKL-40 shows strong links to conditions like osteoarthritis and certain cancers. In osteoarthritis elevated levels of CHI3L1 reflect increased cartilage degradation while in oncology it correlates with tumor angiogenesis and metastasis potential. Its connection with proteins such as matrix metalloproteinases highlights its role in disease mechanisms highlighting its potential as a biomarker for early detection and disease progression monitoring.
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4 product images
Sandwich ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (ab238262)
Mouse CHI3L1 Competitor Std Curve Comparison.
Standard Curve comparison between Mouse CHI3L1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (ab238262)
Example of mouse CHI3L1 standard curve in Sample Diluent NS. The CHI3L1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (ab238262)
Interpolated concentrations of native CHI3L1 in mouse serum and plasma samples. The concentrations of CHI3L1 were measured in duplicates, interpolated from the CHI3L1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 5%, plasma (EDTA) 5% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CHI3L1 concentration was determined to be 58.00 ng/mL in serum, 28.30 ng/mL in plasma (citrate), 52.70 pg/mL in plasma (EDTA), and 82.22 ng/mL in plasma (Heparin).
Sandwich ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (ab238262)
Interpolated concentrations of native CHI3L1 in mouse supernatant samples. The concentrations of CHI3L1 were measured in duplicates, interpolated from the CHI3L1 standard curves and corrected for sample dilution. Undiluted samples are as follows: mouse heart supernatant 50%, mouse liver supernatant 50%, mouse lung supernatant 2.5%, and mouse heart supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CHI3L1 concentration was determined to be 6.31 ng/mL in mouse heart supernatant, 1.65 ng/mL in mouse liver supernatant), 157.17 pg/mL in mouse lung supernatant, and 23.91 ng/mL in mouse spleen supernatant.
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