Mouse YKL-40 / CHI3L1 ELISA Kit
- Recombinant
- SimpleStep
- What is this?
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(2 Publications)
Mouse YKL-40 / CHI3L1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse YKL-40 / CHI3L1 with a sensitivity of 3.17 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Brp39, Chil1, Chi3l1, Chitinase-3-like protein 1, BRP39 protein, Breast regression protein 39, Cartilage glycoprotein 39, CGP-39, GP-39
- ELISA
Supplier Data
ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (AB238262)
- sELISA
Supplier Data
Sandwich ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (AB238262)
Mouse CHI3L1 Competitor Std Curve Comparison.
Standard Curve comparison between Mouse CHI3L1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (AB238262)
Example of mouse CHI3L1 standard curve in Sample Diluent NS. The CHI3L1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (AB238262)
Interpolated concentrations of native CHI3L1 in mouse supernatant samples. The concentrations of CHI3L1 were measured in duplicates, interpolated from the CHI3L1 standard curves and corrected for sample dilution. Undiluted samples are as follows : mouse heart supernatant 50%, mouse liver supernatant 50%, mouse lung supernatant 2.5%, and mouse heart supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CHI3L1 concentration was determined to be 6.31 ng/mL in mouse heart supernatant, 1.65 ng/mL in mouse liver supernatant), 157.17 pg/mL in mouse lung supernatant, and 23.91 ng/mL in mouse spleen supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (AB238262)
Interpolated concentrations of native CHI3L1 in mouse serum and plasma samples. The concentrations of CHI3L1 were measured in duplicates, interpolated from the CHI3L1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, plasma (citrate) 5%, plasma (EDTA) 5% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CHI3L1 concentration was determined to be 58.00 ng/mL in serum, 28.30 ng/mL in plasma (citrate), 52.70 pg/mL in plasma (EDTA), and 82.22 ng/mL in plasma (Heparin).
Reactivity data
Product details
How the assay works
Mouse YKL-40 / CHI3L1 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse YKL-40 / CHI3L1 ELISA Kit ab238262 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-YKL-40/CHI3L1 antibody [EPR21754-8] - BSA and Azide free (Capture) ab245069
- Anti-YKL-40/CHI3L1 antibody [EPR21754-27] - BSA and Azide free (Detector) ab245069
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
YKL-40 plays significant roles in tissue remodeling and inflammation. It does not form part of a larger complex by itself but influences several cellular processes. This protein affects cell proliferation migration and survival making it involved in both normal physiological processes and pathological conditions. CHI3L1 also exhibits a connection to the immune response where it modulates macrophage activity and interacts with other immune cells.
Pathways
YKL-40 is integral to the inflammatory and extracellular matrix pathways. It associates with proteins such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-?) influencing signaling cascades that modulate inflammation. Through these interactions CHI3L1 becomes a mediator of connective tissue metabolism and immune reactions in various pathophysiological states.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (2)
Recent publications for all applications. Explore the full list and refine your search
Brain, behavior, & immunity - health 26:100555 PubMed36457825
2022
Applications
Unspecified application
Species
Unspecified reactive species
Biomedicines 10: PubMed35625766
2022
Applications
Unspecified application
Species
Unspecified reactive species
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