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AB213756

N Cadherin ELISA Kit

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(3 Publications)

N Cadherin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of N Cadherin in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

CD325, CDHN, NCAD, CDH2, Cadherin-2, CDw325, Neural cadherin, N-cadherin

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Sandwich ELISA - N Cadherin ELISA Kit (AB213756)
  • sELISA

Supplier Data

Sandwich ELISA - N Cadherin ELISA Kit (AB213756)

N-Cadherin ELISA Kit (ab213756) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 20 pg/mL

Range

156 - 10000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The N Cadherin Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213756) is designed for the quantitative measurement of Human N-Cadherin in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for Cadherin-2/N-Cadherin has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: D160-A724) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cadherin-2/N Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Cadherin-2/N-Cadherin amount of sample captured in plate.

N Cadherin, also known as Cadherin-2 (CDH2), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. N-Cadherin is expressed in the brain, skeletal and cardiac muscle. N Cadherin is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "12.5 ng/mL", "standardDeviation": "0.675", "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "26.4 ng/mL", "standardDeviation": "1.61", "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "40.7 ng/mL", "standardDeviation": "2.768", "coefficientOfVariability": "6.8" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "9.6 ng/mL", "standardDeviation": "0.365", "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "22.4 ng/mL", "standardDeviation": "0.963", "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "38.2 ng/mL", "standardDeviation": "1.986", "coefficientOfVariability": "5.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213756-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213756-CMP10", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- Human Cadherin-2 antibody", "number":"AB213756-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Cadherin-2 coated Microplate (12 x 8 wells)", "number":"AB213756-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213756-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213756-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213756-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213756-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Human Cadherin-2 standard", "number":"AB213756-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213756-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213756-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213756-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.
Biological function summary

N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.

Pathways

N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.

N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.
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Product protocols

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Target data

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone : upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
See full target information CDH2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 9:9820 PubMed31285444

2019

MicroRNA-212-3p inhibits the Proliferation and Invasion of Human Hepatocellular Carcinoma Cells by Suppressing CTGF expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Qing Chen,Yang-Liu Ou,Zhi-Ping Huang,Yong-Gang Hong,Yuan-Ping Tao,Zhen-Guang Wang,Jun-Sheng Ni,Li-Qiang Hao,Hui Lin

Journal of cellular and molecular medicine 23:1541-1552 PubMed30597727

2018

MiR-552 promotes the proliferation, migration and EMT of hepatocellular carcinoma cells by inhibiting AJAP1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqing Qu,Xinyuan Wen,Keli Su,Wei Gou

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 98:102-110 PubMed29247949

2017

Long non-coding RNA OIP5-AS1 functions as an oncogene in lung adenocarcinoma through targeting miR-448/Bcl-2.

Applications

WB

Species

Human

Jun Deng,Huan Deng,Chunfeng Liu,Yujia Liang,Songping Wang
View all publications
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