NEFL ELISA Kit is a single-wash 90-min Simplestep used to quantify NEFL with a sensitivity of 10.92 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human, Mouse, Rat
- Range
- 93.75 - 6000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 10.92 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
Alternative names
NF68, NFL, NEFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein
What's included?
Recommended products
NEFL ELISA Kit is a single-wash 90-min Simplestep used to quantify NEFL with a sensitivity of 10.92 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human, Mouse, Rat
- Range
- 93.75 - 6000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 10.92 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 8 | C.V. 7.8 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. 11 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Tissue Extracts | Average % = 95 | Range 84 - 102 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
NEFL ELISA Kit (ab288182) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NEFL protein in tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate NEFL with 10.92 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NEFL also known as neurofilament light polypeptide is a component of the cytoskeleton of neurons. It is one of the subunits of neurofilaments which also include medium and heavy chains. NEFL has a molecular mass of about 61.5 kDa. The protein is predominantly expressed in neurons throughout the central and peripheral nervous systems providing structural support and regulating axon diameter.
- Biological function summary
Neurofilament light polypeptide forms heteropolymers with other neurofilament proteins contributing to the assembly of stable neurofilament fibers. These fibers maintain neuronal integrity and proper conductance velocities of nerve signals. NEFL interacts closely with neurofilament medium and heavy chains playing a role in the structural framework of the neuron. This enables efficient transmission of electrical impulses along axons.
- Pathways
NEFL plays a significant role in the neurofilament pathway which is critical for neuronal cytoskeleton assembly and axonal transport. This pathway involves proteins such as NEFM (neurofilament medium) and NEFH (neurofilament heavy). NEFL's involvement in maintaining axonal caliber connects it to important signaling cascades like the axon guidance and neurotransmitter release pathways enhancing neuronal communication.
- Associated diseases and disorders
Defects in NEFL can lead to neurological conditions such as Charcot-Marie-Tooth disease type 2E and amyotrophic lateral sclerosis. Mutations or disruptions in NEFL impact axonal transport and neurofilament assembly contributing to neurodegeneration. NEFL is often studied alongside proteins like peripherin which can also exhibit mutations linked to similar neuropathies underlining its importance in neural health.
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2 product images
Sandwich ELISA - NEFL ELISA Kit (ab288182)
Interpolated concentrations of human NEFL in human brain tissue extract, mouse brain tissue extract, rat brain tissue extract.
Interpolated concentration of native NEFL was measured in duplicate at different sample concentrations. Undiluted samples are as follows: human brain extract 10 ug/mL, mouse brain extract 5 ug/mL, rat brain extract 10 ug/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - NEFL ELISA Kit (ab288182)
Example of human NEFL standard curve in 1X Cell Extraction Buffer PTR. Example of human NEFL standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Product protocols
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