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AB285276

Neomycin ELISA Kit

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Neomycin ELISA Kit is a Competitive ELISA for the measurement of Neomycin in in Cell/Tissue Extracts, Biofluids samples.
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ELISA - Neomycin ELISA Kit (AB285276)
  • ELISA

Unknown

ELISA - Neomycin ELISA Kit (AB285276)

Neomycin ELISA Kit.

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Tissue sections, Milk

Assay type

Competitive

Sensitivity

= 0.1 ng/mL

Range

0.1 - 8.1 ng/mL

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Neomycin is an aminoglycoside antibiotic found in many topical medications such as creams, ointments, and eyedrops. It is typically used as a topical preparation, such as Neosporin. It can also be given orally, where it is usually combined with other antibiotics. Neomycin, like other aminoglycosides, has been shown to be ototoxic causing tinnitus, hearing loss, and vestibular problems in a small number of patients. Standard techniques/instruments (HPLC or GC-MS) are utilized to detect neomycin. However, these techniques are complex, expensive, laborious, and time-consuming. Immunoassay techniques, such as ELISAs are commonly preferred as a simple, reliable and rapid method for the quantification of neomycin in various samples. The Neomycin ELISA Kit (ab285276, E4611) is a competitive-based ELISA that can be used for the determination of this antibiotic in tissue, honey, milk and milk powder.

This product is manufactured by BioVision, an Abcam company and was previously called E4611 Neomycin ELISA Kit. E4611-100 is the same size as the 96 test size of ab285276.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neomycin commonly part of the aminoglycoside class of antibiotics directly targets bacterial ribosomes. It binds to the 30S ribosomal subunit interfering with protein synthesis. This disrupts the bacterial translation process leading to translational errors during protein production. Neomycin has a molecular mass of around 614.64 Da. It is expressed and used in many areas especially in laboratories and clinical settings to suppress bacterial growth in cultures and samples.
Biological function summary

The antibiotic action of neomycin related significantly to its binding affinity for ribosomal RNA within bacterial cells. This binding inhibits the translational activity effectively stopping bacterial cell growth and replication. Neomycin is not a part of a protein complex but functions by interacting with ribosomal RNA. Its broad-spectrum effects make it useful against many Gram-positive and Gram-negative bacteria.

Pathways

Neomycin impacts the protein translation and synthesis pathway within bacterial cells. By binding to the 30S ribosomal subunit neomycin halts the accuracy of mRNA translation affecting proteins within the bacterial cell necessary for their survival. It shares similarities with other aminoglycosides like gentamicin which also target protein synthesis pathways. These compounds utilize similar interventions to disrupt bacterial translation and growth.

Neomycin effectively treats infections caused by susceptible bacteria particularly skin and ocular types. It also finds use in preventing infections during bowel surgery due to its effectiveness in reducing bowel flora. Adverse effects like nephrotoxicity and ototoxicity relate to interactions with human cellular components particularly the proteins in the ear and kidney cells. Similar nephrotoxic effects are seen with other aminoglycosides including tobramycin indicating a class effect of this type of medication.
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Product protocols

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Target data

See full target information Neomycin
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websiteProtocolBooklet
en
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