Nitrotyrosine ELISA Kit is a Competitive ELISA kit for the measurement of Nitrotyrosine in Urine, Plasma, Serum, Other biological fluids, Cell Lysate samples.
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- Cardiovascular research 119:2190-22012023Chronic SIRT1 supplementation in diabetic mice improves endothelial function by suppressing oxidative stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKangmin Yang,Srividya Velagapudi,Alexander Akhmedov,Simon Kraler,Tetiana Lapikova-Bryhinska,Martin O Schmiady,Xiaoping Wu,Leiluo Geng,Giovanni G Camici,Aimin Xu,Thomas F LüscherPubMed 37401647
- International journal of environmental research and public health 20:2023Soluble ST2 as a New Oxidative Stress and Inflammation Marker in Metabolic Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIgnacio Roy,Eva Jover,Lara Matilla,Virginia Alvarez,Amaya Fernández-Celis,Maite Beunza,Elena Escribano,Alicia Gainza,Rafael Sádaba,Natalia López-AndrésPubMed 36767947
- Communications biology 5:9292022Paternal low protein diet perturbs inter-generational metabolic homeostasis in a tissue-specific manner in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHannah L Morgan,Samuel Furse,Irundika H K Dias,Kiran Shabir,Marcos Castellanos,Iqbal Khan,Sean T May,Nadine Holmes,Matthew Carlile,Fei Sang,Victoria Wright,Albert Koulman,Adam J WatkinsPubMed 36075960
- European journal of applied physiology 122:1949-19642022Blood oxidative stress biomarkers in women: influence of oral contraception, exercise, and N-acetylcysteine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarlee M Quinn,Llion Roberts,Amanda J Cox,David N Borg,Evan N Pennell,Daniel R McKeating,Joshua J Fisher,Anthony V Perkins,Clare MinahanPubMed 35674828
- Brain, behavior, & immunity - health 21:1004352022Antroquinonol administration in animal preclinical studies for Alzheimer's disease (AD): A new avenue for modifying progression of AD pathophysiology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFernandez Francesca,Aust Caitlin,Lye Sarah,Griffiths Lyn RobynPubMed 35252893
- Cell biology international 46:381-3902022Overexpression of NDUFV1 alleviates renal damage by improving mitochondrial function in unilateral ureteral obstruction model mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuiting Wang,Chen Kairen,Lu Li,Lingling Zhang,Haifeng Gong,Xinzhong HuangPubMed 34936716
- Southern African journal of HIV medicine 22:12982021Arterial stiffness is associated with oxidative stress and endothelial activation among persons with treated HIV in Zambia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTheresa Chikopela,Fastone Goma,Longa Kaluba,Wilbroad Mutale,Chris Guure,Douglas C Heimburger,John R KoethePubMed 34858654
- American journal of physiology. Heart and circulatory physiology 321:H309-H3172021Deficiency in ST2 signaling ameliorates RSV-associated pulmonary hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuan D Vu,Jordy Saravia,Sridhar Jaligama,Rajshri V Baboeram Panday,Ryan D Sullivan,Salvatore Mancarella,Stephania A Cormier,Dai KimuraPubMed 34170196
- Beni-Suef University journal of basic and applied sciences 10:2021Endothelial dysfunction and body mass index: is there a role for plasma peroxynitrite?Applications:Unspecified applicationReactive species:Unspecified reactive speciesTheresa Chikopela,Douglas C Heimburger,Longa Kaluba,Pharaoh Hamambulu,Newton Simfukwe,Wilbroad Mutale,John R Koethe,Fastone GomaPubMed 33569505
- The Journal of nutrition 150:2460-24682020Nitrate-Rich Beetroot Juice Reduces Blood Pressure in Tanzanian Adults with Elevated Blood Pressure: A Double-Blind Randomized Controlled Feasibility Trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMario Siervo,Oliver Shannon,Navneet Kandhari,Meghna Prabhakar,William Fostier,Christina Köchl,Jane Rogathi,Gloria Temu,Blossom C M Stephan,William K Gray,Irene Haule,Stella-Maria Paddick,Blandina T Mmbaga,Richard WalkerPubMed 32729923
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Plasma, Serum, Other biological fluids, Cell Lysate
- Assay type
- Competitive
- Range
- 62.5 - 8000 nM
- Assay time
- 2h
- Sensitivity
- = 50 nM
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
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Nitrotyrosine ELISA Kit is a Competitive ELISA kit for the measurement of Nitrotyrosine in Urine, Plasma, Serum, Other biological fluids, Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Plasma, Serum, Other biological fluids, Cell Lysate
- Assay type
- Competitive
- Range
- 62.5 - 8000 nM
- Assay time
- 2h
- Assay Platform
- Microplate
- Sensitivity
- = 50 nM
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 30 | C.V. < 10 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 30 | C.V. < 15 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's Nitrotyrosine in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab210603) is designed for the competitive measurement of Free Nitrotyrosine protein in plasma, serum, cell lysates, urine, and other sample matrices. The ELISA utilizes an Nitrotyrosine-coated plate and an HRP-conjugated antibody for detection which allows for an assay range of 62.5 to 8000 nM Free Nitrotyrosine, with a sensitivity of 50 nM. The other highlights of this kit are a quick incubation time of 60 minutes, stable reagents, and an easy to use protocol.
Nitrotyrosine has been identified as a marker of inflammation and NO production. Nitrotyrosine is formed in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production. Since nitrotyrosine is a stable end product of peroxynitrite oxidation, assessment of its plasma concentration may be useful as a marker of NO-dependent damage in vivo. Since NOX is only an indicator for enhanced NO production, protein associated nitrotyrosine might be a more suitable marker for damage induced by reactive nitrogen intermediates derived from NO. Furthermore, most proteins have a longer half-life in the circulation than NOX levels. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock. In normal plasma low, undetectable, levels of nitrotyrosine are present.
Nitrosylation of the amino acid tyrosine occurs both for free tyrosine and for protein bound tyrosine.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nitrotyrosine also known as 3-nitrotyrosine is a marker for nitrosative stress and oxidative damage. It comes from the nitration of tyrosine an amino acid primarily through the action of reactive nitrogen species. Nitrotyrosine can be found in proteins and its molecular mass varies depending on the protein context. It is present across various tissues and fluid samples within the body particularly where high oxidative stress occurs.
- Biological function summary
Nitrotyrosine acts as an indicator of cellular damage and protein modification due to stress conditions. When proteins have nitrotyrosine residues their function might change which can lead to cellular signaling disruption. Nitrotyrosine is not usually part of a specific complex but it affects proteins and enzymes by altering their activity and stability contributing to larger stress response mechanisms.
- Pathways
Proteins modified by nitrotyrosine are involved in cellular pathways related to oxidative stress and inflammation. Particularly pathways like the nitric oxide synthase pathway and the inflammatory response pathway include nitrotyrosine-modified proteins. It is related to proteins such as nitric oxide synthase (NOS) and superoxide dismutase (SOD) important in managing oxidative balance and stress responses in cells.
- Associated diseases and disorders
Nitrotyrosine is significantly linked to diseases related to oxidative stress such as cardiovascular disease and neurodegenerative disorders. These conditions often have increased levels of nitrotyrosine indicating heightened oxidative damage. Through these diseases nitrotyrosine connects with proteins like amyloid-beta in neurodegenerative disorders and endothelial nitric oxide synthase (eNOS) in cardiovascular diseases reflecting the impact of oxidative stress on these proteins.
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2 product images
Competitive ELISA - Nitrotyrosine ELISA Kit (ab210603)
Standard Curve Nitrotyrosine ELISA Kit ab210603
Competitive ELISA - Nitrotyrosine ELISA Kit (ab210603)
Example of Nitrotyrosine standard curve.
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Product protocols
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