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AB237651

Nivolumab ELISA Kit

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(1 Publication)

Nivolumab ELISA Kit is a sandwich ELISA designed to quantify Nivolumab with a sensitivity of 30 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 30 - 1000 ng/mL

View Alternative Names

Opdivo

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Sandwich ELISA - Nivolumab ELISA Kit (AB237651)
  • sELISA

Supplier Data

Sandwich ELISA - Nivolumab ELISA Kit (AB237651)

Example of Nivolumab standard curve.

Typical Standard Curve : This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 30 ng/mL

Range

30 - 1000 ng/mL

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Nivolumab ELISA Kit ab237651 is a sandwich ELISA to measure Nivolumab in serum, plasma with a sensitivity of 30 ng/ml.

How the assay works

Note: Nivolumab ELISA kit ab237651 is intended for Research Use Only, not for use in diagnostic procedures.

This kit is a sandwich based ELISA kit. The color developed is proportional to the amount of Nivolumab in the sample or standard. Results of samples can be determined directly using the standard curve.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Nivolumab ELISA Kit ab237651 protocol summary

1. Add Assay buffer to each well and standard or sample to appropriate wells. Incubate
2. Wash
3. Add HRP-conjugate to each well. Incubate
4. Wash
5. Add TMB Substrate. Incubate the plate
6. Add Stop Solution and read OD at 450 nm

This product is manufactured by BioVision, an Abcam company and was previously called E4382 BioSim™ Nivolumab (Human) ELISA Kit. E4382-100 is the same size as the 96 test size of ab237651.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 15" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 15" } ]
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Recovery

[ { "sample": "Serum", "range": "85 - 115 %", "average": null } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information Nivolumab
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:4698 PubMed38844770

2024

Ultrasound-mediated delivery of doxorubicin to the brain results in immune modulation and improved responses to PD-1 blockade in gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Víctor A Arrieta,Andrew Gould,Kwang-Soo Kim,Karl J Habashy,Crismita Dmello,Gustavo I Vázquez-Cervantes,Irina Palacín-Aliana,Graysen McManus,Christina Amidei,Cristal Gomez,Silpol Dhiantravan,Li Chen,Daniel Y Zhang,Ruth Saganty,Meghan E Cholak,Surya Pandey,Matthew McCord,Kathleen McCortney,Brandyn Castro,Rachel Ward,Miguel Muzzio,Guillaume Bouchoux,Carole Desseaux,Michael Canney,Alexandre Carpentier,Bin Zhang,Jason M Miska,Maciej S Lesniak,Craig M Horbinski,Rimas V Lukas,Roger Stupp,Catalina Lee-Chang,Adam M Sonabend
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com