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AB237651

Nivolumab ELISA Kit

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Nivolumab ELISA Kit is a sandwich ELISA designed to quantify Nivolumab with a sensitivity of 30 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 30 - 1000 ng/mL

View Alternative Names

Opdivo

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Sandwich ELISA - Nivolumab ELISA Kit (AB237651)
  • sELISA

Supplier Data

Sandwich ELISA - Nivolumab ELISA Kit (AB237651)

Example of Nivolumab standard curve.

Typical Standard Curve : This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 30 ng/mL

Range

30 - 1000 ng/mL

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Nivolumab ELISA Kit ab237651 is a sandwich ELISA to measure Nivolumab in serum, plasma with a sensitivity of 30 ng/ml.

How the assay works

Note: Nivolumab ELISA kit ab237651 is intended for Research Use Only, not for use in diagnostic procedures.

This kit is a sandwich based ELISA kit. The color developed is proportional to the amount of Nivolumab in the sample or standard. Results of samples can be determined directly using the standard curve.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Nivolumab ELISA Kit ab237651 protocol summary

1. Add Assay buffer to each well and standard or sample to appropriate wells. Incubate
2. Wash
3. Add HRP-conjugate to each well. Incubate
4. Wash
5. Add TMB Substrate. Incubate the plate
6. Add Stop Solution and read OD at 450 nm

This product is manufactured by BioVision, an Abcam company and was previously called E4382 BioSim™ Nivolumab (Human) ELISA Kit. E4382-100 is the same size as the 96 test size of ab237651.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 15" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 15" } ]
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Recovery

[ { "sample": "Serum", "range": "85 - 115 %", "average": null } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab237651-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"Plate sealers", "number":"AB237651-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB237651-CMP03", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Nivolumab Standard S6", "number":"AB237651-CMP10", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Nivolumab Standard S2", "number":"AB237651-CMP06", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Nivolumab Standard S4", "number":"AB237651-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB substrate", "number":"AB237651-CMP14", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Nivolumab Standard S3", "number":"AB237651-CMP07", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Nivolumab Standard S7", "number":"AB237651-CMP11", "productcode":"" }, { "size":"2 x 50 mL", "name":"Assay Buffer", "number":"AB237651-CMP01", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Nivolumab Standard S5", "number":"AB237651-CMP09", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Nivolumab Standard S1", "number":"AB237651-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Wash buffer (20X)", "number":"AB237651-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB237651-CMP15", "productcode":"" }, { "size":"1 x 12 mL", "name":"HRP-conjugate Probe", "number":"AB237651-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Nivolumab
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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websiteProtocolBooklet
en
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Associated Products

Select an associated product type
Alternative Product
Assay Kits

AB237665

anti-Nivolumab ELISA Kit

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nivolumab-elisa-kit-ab237665

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com