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AB285284

Overall Exosome Capture and Quantification ELISA Assay Kit

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Overall Exosome Capture and Quantification ELISA Assay Kit is a Sandwich ELISA for the measurement of Overall Exosome Capture and Quantification ELISA Assay Kit in in Biofluids samples.
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Sandwich ELISA - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284)
  • sELISA

Supplier Data

Sandwich ELISA - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284)

CD9 Titration of COLO1 (1) and plasma (2) exosomes. Example of standard curves obtained. Exosome standard preparation derived from (1) COLO1 Cell Culture Supernatant and (2) Plasma of healthy donors. This standard curve is for demonstration only. For quantification purposes, a standard curve must be obtained with every assay.

Sandwich ELISA - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284)
  • sELISA

Supplier Data

Sandwich ELISA - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284)

CD9 titration of plasma healthy donor exosome standards.

Western blot - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284)
  • WB

Supplier Data

Western blot - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284)

CD9 marker detection by Western Blotting on lyophilized exosomes from human plasma and the recombinant CD9 protein.

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Key facts

Sample types

Plasma

Assay type

Sandwich

Range

0.78125 - 50 µg

Assay Platform

Microplate (12 x 8 well strips)

Product details

Overall Exosome Capture and Quantification ELISA Assay Kit ab285284 (previously called ExoQuant Overall Exosome Capture and Quantification ELISA Assay Kit (Human Plasma, Colorimetric), K1201) is a patented double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes.

It is a successful platform for exosomes quantification and characterization from small amount of human biological fluids (plasma, serum, urine, saliva) or cell media. It may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. The kit consists of ELISA plates pre-coated with proprietary pan-exosome antibodies enabling specific capture of exosomes from different biological samples, including cell culture supernatants and human biological fluids. Quantification and characterization of exosomal proteins is subsequently performed using appropriate detection antibodies against exosome associated antigens that can be for either generic or cell/tissue-specific exosomes. The kit contains Kit contains Lyophilized Exosome Standards for assay calibration. Lyophilized Exosome Standards, characterized for protein content and particle number allow the quantification of unknown sample by a standard calibration curve. Transparent and white plates are available depending on the downstream detection approach (colorimetric and luminometric respectively).

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 13" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Product protocols

Target data

websiteProtocolBooklet
en

Product promise

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