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Overall Exosome Capture and Quantification ELISA Assay Kit is a Sandwich ELISA kit for the measurement of Overall Exosome Capture and Quantification ELISA Assay Kit in Plasma samples.

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Images

Sandwich ELISA - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284), expandable thumbnail
  • Western blot - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284), expandable thumbnail
  • Sandwich ELISA - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284), expandable thumbnail

Key facts

Sample types
Plasma
Assay type
Sandwich
Range
0.78125 - 50 µg

What's included?

96 Tests
Components
25X Wash buffer
1 x 25 mL
Exosome Standards
2 x 1 Vial
HRP-Conjugate
1 x 1 Vial
Immunoplate (Transparent)
1 x 1 Unit
Primary antibody
1 x 20 µL
Sample buffer
2 x 10 mL
Stop Solution
1 x 10 mL
Substrate chromogenic solutions
1 x 10 mL

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Overall Exosome Capture and Quantification ELISA Assay Kit is a Sandwich ELISA kit for the measurement of Overall Exosome Capture and Quantification ELISA Assay Kit in Plasma samples.

Key facts

Sample types
Plasma
Assay type
Sandwich
Range
0.78125 - 50 µg
Assay Platform
Microplate (12 x 8 well strips)

Precision

Intra assay

Sample
Overall
n
0
C.V.
< 10

Inter assay

Sample
Overall
n
0
C.V.
< 13

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Overall Exosome Capture and Quantification ELISA Assay Kit ab285284 (previously called ExoQuant Overall Exosome Capture and Quantification ELISA Assay Kit (Human Plasma, Colorimetric), K1201) is a patented double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes.

It is a successful platform for exosomes quantification and characterization from small amount of human biological fluids (plasma, serum, urine, saliva) or cell media. It may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. The kit consists of ELISA plates pre-coated with proprietary pan-exosome antibodies enabling specific capture of exosomes from different biological samples, including cell culture supernatants and human biological fluids. Quantification and characterization of exosomal proteins is subsequently performed using appropriate detection antibodies against exosome associated antigens that can be for either generic or cell/tissue-specific exosomes. The kit contains Kit contains Lyophilized Exosome Standards for assay calibration. Lyophilized Exosome Standards, characterized for protein content and particle number allow the quantification of unknown sample by a standard calibration curve. Transparent and white plates are available depending on the downstream detection approach (colorimetric and luminometric respectively).

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Overall Exosome Capture and Quantification ELISA Assay Kit (ab285284), expandable thumbnail

    Sandwich ELISA - Overall Exosome Capture and Quantification ELISA Assay Kit (ab285284)

    CD9 Titration of COLO1 (1) and plasma (2) exosomes. Example of standard curves obtained. Exosome standard preparation derived from (1) COLO1 Cell Culture Supernatant and (2) Plasma of healthy donors. This standard curve is for demonstration only. For quantification purposes, a standard curve must be obtained with every assay.

  • Western blot - Overall Exosome Capture and Quantification ELISA Assay Kit (ab285284), expandable thumbnail

    Western blot - Overall Exosome Capture and Quantification ELISA Assay Kit (ab285284)

    CD9 marker detection by Western Blotting on lyophilized exosomes from human plasma and the recombinant CD9 protein.

  • Sandwich ELISA - Overall Exosome Capture and Quantification ELISA Assay Kit (ab285284), expandable thumbnail

    Sandwich ELISA - Overall Exosome Capture and Quantification ELISA Assay Kit (ab285284)

    CD9 titration of plasma healthy donor exosome standards.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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