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AB273256

Ovine IL-17A ELISA Kit

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Ovine IL-17A ELISA Kit is a Sandwich ELISA for the measurement of Ovine IL-17A in Sheep in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Ovine IL-17A ELISA Kit (AB273256)
  • sELISA

Supplier Data

Sandwich ELISA - Ovine IL-17A ELISA Kit (AB273256)

Ovine IL-17A ELISA Kit standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Sheep

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.45 pg/mL

Range

0.41 - 100 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Ovine IL-17A ELISA Kit (ab273256) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Ovine IL-17A in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Ovine IL-17A coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17A present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Ovine IL-17A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17A. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "104 - 121 %", "average": "= 110.1" }, { "sample": "Plasma", "range": "82 - 139 %", "average": "= 105.1" }, { "sample": "Cell culture media", "range": "92 - 105 %", "average": "= 98.12" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273256-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273256-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin Concentrate", "number":"AB273256-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273256-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Ovine IL-17A Antibody", "number":"AB273256-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273256-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Ovine IL-17A Standard (Lyophilized)", "number":"AB273256-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Ovine IL-17A coated Microplate (12 x 8 wells)", "number":"AB273256-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273256-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

See full target information IL17A
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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