Oxytocin ELISA Kit
3
(4 Reviews)
|
(8 Publications)
Oxytocin ELISA Kit is a competitive ELISA designed to quantify Oxytocin with a sensitivity of 15 pg/mL
- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Wide dynamic range - quantifies 15.6 - 1000 pg/ml
View Alternative Names
OT, OT NPI, OXT, Ocytocin, Oxytocin neurophysin I preproprotein, Oxytocin prepro (neurophysin I), Oxytocin-neurophysin 1
- cELISA
Supplier Data
Competitive ELISA - Oxytocin ELISA Kit (AB133050)
Representative Standard Curve using ab133050.
Reactivity data
Product details
Abcam's Oxytocin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Oxytocin in Tissue Culture media, Human Serum, Saliva, Cerebrospinal Fluid and Urine.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Oxytocin antigen and a polyclonal rabbit antibody specific to Oxytocin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Oxytocin captured in the plate.
Oxytocin is a neurohypophysial peptide which is produced in the paraventricular nuclei of the hypothalamus and stored in the posterior pituitary. The molecule consists of nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail. A hormone once thought to be limited to female smooth muscle reproductive physiology, more current findings have determined that oxytocin also functions as a neurotransmitter, may be involved in neuropsychiatric disorders, social/sexual behavior and is important in male reproductive physiology. Oxytocin and the related neurohypophysial peptide, Arg-Vasopressin, maintain renal water and sodium balance.
Highly conserved across species boundaries, oxytocin-like neurohypophysial peptides are substituted primarily at residues 4 and/or 8. In the Oxytocin-like peptide, mesotocin, a common peptide found in some fishes, reptiles, amphibians, marsupials and nonmammalian tetrapods, the leucine at residue 8 is substituted for isoleucine. Acting in classical endocrine fashion, Oxytocin elicits regulatory effects by binding specific cell surface receptors which in turn initiate a secondary intracellular response cascade via a phosphoinositide signaling pathway.
Cross Reactivity
| Compound | % Cross Reactivity |
| Mesotocin | 7.0 |
| Oxytocin | 100 |
| Arg8-Vasotocin | 7.5 |
| Ser4,Ile8-Oxytocin | <0.2 |
| TRH | <0.2 |
| Somatostatin | <0.2 |
| Met-Enkephalin | <0.2 |
| VIP | <0.2 |
| Lys8-Vasopressin | <0.2 |
| Arg8-Vasopressin | <0.2 |
| α−ANP | <0.2 |
| Growth Hormone | <0.2 |
| Tocinoic acid | <0.2 |
| Melanostatin | <0.2 |
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Oxytocin plays several significant roles in the body. It is involved in reproductive and social behaviors such as childbirth and lactation. During childbirth oxytocin induces uterine contractions facilitating the labor process. It also promotes milk ejection during breastfeeding by mediating the contraction of myoepithelial cells surrounding the mammary glands. Furthermore oxytocin contributes to social bonding and modulates emotional responses. It acts both in the central nervous system and peripherally affecting various physiological processes.
Pathways
Oxytocin is an important player in the neuroendocrine system especially in the pathways regulating labor and lactation. It functions closely with other neuropeptides and hormones such as vasopressin which also shares sequence homology and structural similarities. Oxytocin influences the MAPK/ERK signaling pathway which impacts cellular growth and differentiation. These pathways highlight oxytocin's role not only in reproductive events but also in the broader context of cell signaling networks within the body.
Product protocols
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Target data
Publications (8)
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BMC psychology 10:36 PubMed35193693
2022
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Annals of the New York Academy of Sciences 1502:121-131 PubMed34273130
2021
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Translational psychiatry 10:356 PubMed33077706
2020
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Molecular medicine reports 22:1921-1931 PubMed32705167
2020
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Nature biomedical engineering 4:544-559 PubMed32341538
2020
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Beneficial microbes 10:653-659 PubMed31157556
2019
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Scientific reports 8:13084 PubMed30166555
2018
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Personality and mental health 11:91-100 PubMed28397403
2017
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Human
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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