Skip to main content

Oxytocin ELISA Kit is a Competitive ELISA kit for the measurement of Oxytocin in Human in Cerebral Spinal Fluid, Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum, Tissue Culture Media samples.


Images

Competitive ELISA - Oxytocin ELISA Kit (AB133050), expandable thumbnail

Publications

Key facts

Detection method
Colorimetric
Sample types
Cerebral Spinal Fluid, Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum, Tissue Culture Media
Assay type
Competitive
Reactive species
Human
Range
15.6 - 1000 pg/mL
Assay time
25h
Sensitivity
= 15 pg/mL

Reactivity data

Application
cELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Alternative names

OT, OT NPI, OXT, Ocytocin, Oxytocin neurophysin I preproprotein, Oxytocin prepro (neurophysin I), Oxytocin-neurophysin 1

What's included?

1 x 96 Tests
Components
20X Wash Buffer Concentrate
1 x 27 mL
Assay Buffer
1 x 27 mL
Goat anti-rabbit IgG Microplate (12 x 8 wells)
1 x 1 Unit
Oxytocin Alkaline Phosphatase Conjugate
1 x 5 mL
Oxytocin Antibody
1 x 5 mL
Oxytocin Standard
1 x 0.5 mL
Plate Sealer
1 x 1 Unit
Stop Solution
1 x 5 mL
pNpp Substrate
1 x 20 mL

Recommended products

Oxytocin ELISA Kit is a Competitive ELISA kit for the measurement of Oxytocin in Human in Cerebral Spinal Fluid, Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum, Tissue Culture Media samples.

Key facts

Detection method
Colorimetric
Sample types
Cerebral Spinal Fluid, Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum, Tissue Culture Media
Assay type
Competitive
Reactive species
Human
Range
15.6 - 1000 pg/mL
Assay time
25h
Assay Platform
Microplate
Sensitivity
= 15 pg/mL

Precision

Intra assay

Sample
High
n
20
mean
363.7 pg/mL
SD
-
C.V.
13.3
Sample
Low
n
20
mean
39.9 pg/mL
SD
-
C.V.
12.6
Sample
Media
n
20
mean
121.4 pg/mL
SD
-
C.V.
10.2

Inter assay

Sample
High
n
17
mean
397.2 pg/mL
SD
-
C.V.
11.8
Sample
Low
n
17
mean
47 pg/mL
SD
-
C.V.
20.9
Sample
Media
n
17
mean
145.1 pg/mL
SD
-
C.V.
16.5

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 104
Range
Sample type
Urine
Average %
= 111
Range
Sample type
Saliva
Average %
= 90
Range

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

Abcam's Oxytocin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Oxytocin in Tissue Culture media, Human Serum, Saliva, Cerebrospinal Fluid and Urine.

A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Oxytocin antigen and a polyclonal rabbit antibody specific to Oxytocin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Oxytocin captured in the plate.

Oxytocin is a neurohypophysial peptide which is produced in the paraventricular nuclei of the hypothalamus and stored in the posterior pituitary. The molecule consists of nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail. A hormone once thought to be limited to female smooth muscle reproductive physiology, more current findings have determined that oxytocin also functions as a neurotransmitter, may be involved in neuropsychiatric disorders, social/sexual behavior and is important in male reproductive physiology. Oxytocin and the related neurohypophysial peptide, Arg-Vasopressin, maintain renal water and sodium balance.

Highly conserved across species boundaries, oxytocin-like neurohypophysial peptides are substituted primarily at residues 4 and/or 8. In the Oxytocin-like peptide, mesotocin, a common peptide found in some fishes, reptiles, amphibians, marsupials and nonmammalian tetrapods, the leucine at residue 8 is substituted for isoleucine. Acting in classical endocrine fashion, Oxytocin elicits regulatory effects by binding specific cell surface receptors which in turn initiate a secondary intracellular response cascade via a phosphoinositide signaling pathway.

Cross Reactivity

Compound% Cross Reactivity
Mesotocin7.0
Oxytocin100
Arg8-Vasotocin7.5
Ser4,Ile8-Oxytocin<0.2
TRH<0.2
Somatostatin<0.2
Met-Enkephalin<0.2
VIP<0.2
Lys8-Vasopressin<0.2
Arg8-Vasopressin<0.2
α−ANP<0.2
Growth Hormone<0.2
Tocinoic acid<0.2
Melanostatin<0.2

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Oxytocin also known as the "love hormone" is a peptide consisting of nine amino acids with a molecular mass of approximately 1007 Daltons. It is synthesized in the hypothalamus and secreted by the posterior pituitary gland. Additionally oxytocin is expressed in other tissues including the uterus ovaries and even the heart. Its mechanical function involves binding to its receptor the oxytocin receptor (OXTR) which is a G-protein-coupled receptor and triggering intracellular signaling cascades that lead to calcium mobilization muscle contraction and other cellular responses.

Biological function summary

Oxytocin plays several significant roles in the body. It is involved in reproductive and social behaviors such as childbirth and lactation. During childbirth oxytocin induces uterine contractions facilitating the labor process. It also promotes milk ejection during breastfeeding by mediating the contraction of myoepithelial cells surrounding the mammary glands. Furthermore oxytocin contributes to social bonding and modulates emotional responses. It acts both in the central nervous system and peripherally affecting various physiological processes.

Pathways

Oxytocin is an important player in the neuroendocrine system especially in the pathways regulating labor and lactation. It functions closely with other neuropeptides and hormones such as vasopressin which also shares sequence homology and structural similarities. Oxytocin influences the MAPK/ERK signaling pathway which impacts cellular growth and differentiation. These pathways highlight oxytocin's role not only in reproductive events but also in the broader context of cell signaling networks within the body.

Associated diseases and disorders

Oxytocin has links to conditions like autism spectrum disorders (ASD) and postpartum depression. Researchers suggest that alterations in oxytocin levels or its receptor function may contribute to social and emotional dysfunctions found in ASD. In postpartum depression abnormal oxytocin activities are thought to affect mood and emotional bonding between mother and child. Oxytocin interacts with other proteins like the serotonin receptor influencing mood regulation and emotional stability. Understanding oxytocin’s role in these disorders may lead to potential therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Competitive ELISA - Oxytocin ELISA Kit (ab133050), expandable thumbnail

    Competitive ELISA - Oxytocin ELISA Kit (ab133050)

    Representative Standard Curve using ab133050.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com