p38 MAPK alpha ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the semi-quantitative measurement of p38 MAPK alpha Mouse, Human in Tissue Homogenate, Cell Lysate samples.
Images
Publications
Filter by
- Pharmaceuticals (Basel, Switzerland) 15:2022Design, Synthesis, and Biological Evaluation of a Novel VEGFR-2 Inhibitor Based on a 1,2,5-Oxadiazole-2-Oxide Scaffold with MAPK Signaling Pathway Inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMater H Mahnashi,Fardous F El-Senduny,Mohammed Abdulrahman Alshahrani,Mahrous A Abou-SalimPubMed 35215358
- Evidence-based complementary and alternative medicine : eCAM 2021:55187502021Unveiling the Mechanism of Principal Drugs of Lianpu Drink on Chronic Gastritis by Network Pharmacology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuhan Zhou,Xiaohui Xu,Jiangqin Zeng,Zhiyi Liu,Miao Wang,Wenliang LvPubMed 34745289
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Homogenate, Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Mouse, Human
- Range
- 0.781 - 100 ng/mL
- Assay time
- 1h 1m
- Sensitivity
- = 300 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
What's included?
Recommended products
p38 MAPK alpha ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the semi-quantitative measurement of p38 MAPK alpha Mouse, Human in Tissue Homogenate, Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Homogenate, Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Mouse, Human
- Range
- 0.781 - 100 ng/mL
- Assay time
- 1h 1m
- Assay Platform
- Microplate
- Sensitivity
- = 300 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample HeLa Extract | n 6 | mean - | SD - | C.V. 9.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample HeLa Extract | n 3 | mean - | SD - | C.V. 6.4 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's p38 MAPKα in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of p38 MAPKα protein in Human and mouse cells.
The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
As of May 2020, this kit was reformulated with new antibodies to maintain continued long term supply.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
P38 also known as MAPK14 is a member of the mitogen-activated protein kinase (MAPK) family. It functions by phosphorylating various downstream substrates and plays a role in cellular responses. The molecular weight of p38 is approximately 38 kDa. This protein is widely expressed across many tissues including heart brain and lungs. Its activation by stimuli such as cytokines and stress factors helps regulate inflammation and cell cycle control.
- Biological function summary
P38 is involved in several cellular processes such as inflammation cell differentiation and apoptosis. It often functions as part of a MAPK signaling complex where it serves a critical role in transmitting signals from the cell surface to the nucleus. It interacts with upstream kinases for activation and affects cellular responses by phosphorylating transcription factors and other protein kinases. Through experiments using techniques like p38 western blot and alpha ELISA scientists can monitor its activity and understand its role in cellular physiology.
- Pathways
P38 signaling is integral to both the MAPK and NF-kB pathways. It helps mediate several cellular responses including inflammation and stress responses. Within these pathways p38 interacts with other proteins such as JNK and ERK which helps regulate adaptive and innate immune responses. These interactions ensure distinct yet overlapping signaling responses necessary for cellular homeostasis.
- Associated diseases and disorders
P38 plays a role in conditions such as rheumatoid arthritis and cancer. In rheumatoid arthritis p38 contributes to inflammatory processes promoting the production of pro-inflammatory cytokines. In cancer its role varies; while sometimes promoting cancer cell apoptosis it may also aid in tumor survival and proliferation. The protein TNF-alpha often connects indirectly with p38 through inflammatory pathways highlighting its involvement in these diseases.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
ELISA - p38 MAPK alpha ELISA Kit (ab221012)
Cell line analysis for Total p38 MAPKα from 200 µg/mL preparations of cell extracts.
Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).
ELISA - p38 MAPK alpha ELISA Kit (ab221012)
Induction of p38 MAPKα (pT180/Y182) phosphorylation in HeLa cells in response to anisomycin treatment.
HeLa cells were cultured in 96-well tissue culture plates and treated (15 min) with a dose-range of anisomycin before cell lysis. Data from quadruplicate measurements of p38 MAPKα (pT180/Y182) are plotted and compared against Total p38 MAPKα protein levels.
ELISA - p38 MAPK alpha ELISA Kit (ab221012)
Example of p38 MAPKα cell lysate standard curve.
Raw data values are fitted to a four-parameter, variable slope curve (+/- SD).
ELISA - p38 MAPK alpha ELISA Kit (ab221012)
Example of p38 MAPKα recombinant protein standard curve.
Raw data values are fitted to a four-parameter, variable slope curve (+/- SD).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com