p38 MAPK alpha (pT180/pY182) + total p38 MAPK alpha ELISA Kit is a Semi-quantitative ELISA kit for the measurement of p38 MAPK alpha (pT180/pY182) + total p38 MAPK alpha in Mouse, Human in Cell Lysate samples.
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- Nature communications 9:46852018Bi-directional signaling by membrane-bound KitL induces proliferation and coordinates thymic endothelial cell and thymocyte expansion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMario Buono et. al.PubMed 30410062
- Scientific reports 8:165192018Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelanie Gartz et. al.PubMed 30410044
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Mouse, Human
- Assay time
- 5h
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
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p38 MAPK alpha (pT180/pY182) + total p38 MAPK alpha ELISA Kit is a Semi-quantitative ELISA kit for the measurement of p38 MAPK alpha (pT180/pY182) + total p38 MAPK alpha in Mouse, Human in Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Mouse, Human
- Assay time
- 5h
- Assay Platform
- Microplate
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
ab126453 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated p38 alpha protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-p38 alpha (Thr180/Tyr182) and total p38 alpha (help normalize the results of phospho-p38 alpha from different cell lysate being compared). An anti-p38 alpha (Thr180/Tyr182) (half plate, red marker on left side) and anti-total p38 alpha antibody (half plate, black marker on right side) has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated (left side) and total (right side) p38 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti p38 alpha is used to detect phosphorylated or total p38 alpha. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of p38 alpha (Thr180/Tyr182) or total p38 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
P38 alpha also known as MAPK14 is a significant member of the MAP kinase family involved in cellular response to stress signals. This protein has a molecular mass of about 38 kDa and is expressed in various tissues throughout the body. p38 alpha plays an important role in the signal transduction pathways that regulate inflammatory responses and cell differentiation. Its activity is modulated by multiple upstream kinases leading to cell-specific effects that are important for organismal homeostasis.
- Biological function summary
P38 alpha MAPK14 is a part of a larger mitogen-activated protein kinase (MAPK) complex where it serves to mediate signals from external stressors to the appropriate cellular processes. It is particularly active in its roles involving inflammation and apoptosis regulation. The protein interacts with other members of the MAPK family and additional proteins such as TAB1 to conduct these biological signals efficiently.
- Pathways
P38 alpha integrates into the p38 MAPK pathway and the NF-kB signaling pathway which are essential for managing cellular stress responses and inflammatory reactions. It closely interacts with other proteins like MKK3 and MKK6 which are directly upstream regulators phosphorylating and activating p38 MAPK14. This intricate connection allows p38 alpha to execute precise regulation within cellular environments.
- Associated diseases and disorders
P38 alpha MAPK14 is prominently associated with inflammatory diseases such as rheumatoid arthritis and cardiovascular disorders. In these conditions its aberrant activation or expression can lead to pathological inflammation and tissue damage. Additionally p38 alpha’s connection with TNF-alpha in inflammation highlights its relevance in therapeutic targets for related disorders reflecting the significance of its modulation to potentially mitigate disease progression.
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3 product images
Western blot - p38 MAPK alpha (pT180/pY182) + total p38 MAPK alpha ELISA Kit (ab126453)
HeLa cells were treated or untreated with Anisomycin for 10 min at 37°C. Cell lysates were analyzed by Western Blot.
Sandwich ELISA - p38 MAPK alpha (pT180/pY182) + total p38 MAPK alpha ELISA Kit (ab126453)
HeLa cells were treated or untreated with Anisomycin for 10 min at 37°C. Cell lysates were analyzed using this phosphoELISA.
Sandwich ELISA - p38 MAPK alpha (pT180/pY182) + total p38 MAPK alpha ELISA Kit (ab126453)
HeLa cells were treated with Anisomycin at 37°C for 10 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.
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