p38 MAPK alpha (pT180/pY182) + total p38 MAPK alpha ELISA Kit
Be the first to review this product! Submit a review
|
(2 Publications)
- sELISA
Supplier Data
Sandwich ELISA - p38 MAPK alpha (pT180/pY182) + total p38 MAPK alpha ELISA Kit (AB126453)
HeLa cells were treated or untreated with Anisomycin for 10 min at 37°C. Cell lysates were analyzed using this phosphoELISA.
- sELISA
Supplier Data
Sandwich ELISA - p38 MAPK alpha (pT180/pY182) + total p38 MAPK alpha ELISA Kit (AB126453)
HeLa cells were treated with Anisomycin at 37°C for 10 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.
- WB
Supplier Data
Western blot - p38 MAPK alpha (pT180/pY182) + total p38 MAPK alpha ELISA Kit (AB126453)
HeLa cells were treated or untreated with Anisomycin for 10 min at 37°C. Cell lysates were analyzed by Western Blot.
false
Complementary Products
Assay Kits
AB126445
ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit
- 0
- 0
Reactivity data
Product details
ab126453 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated p38 alpha protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-p38 alpha (Thr180/Tyr182) and total p38 alpha (help normalize the results of phospho-p38 alpha from different cell lysate being compared). An anti-p38 alpha (Thr180/Tyr182) (half plate, red marker on left side) and anti-total p38 alpha antibody (half plate, black marker on right side) has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated (left side) and total (right side) p38 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti p38 alpha is used to detect phosphorylated or total p38 alpha. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of p38 alpha (Thr180/Tyr182) or total p38 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
P38 alpha MAPK14 is a part of a larger mitogen-activated protein kinase (MAPK) complex where it serves to mediate signals from external stressors to the appropriate cellular processes. It is particularly active in its roles involving inflammation and apoptosis regulation. The protein interacts with other members of the MAPK family and additional proteins such as TAB1 to conduct these biological signals efficiently.
Pathways
P38 alpha integrates into the p38 MAPK pathway and the NF-kB signaling pathway which are essential for managing cellular stress responses and inflammatory reactions. It closely interacts with other proteins like MKK3 and MKK6 which are directly upstream regulators phosphorylating and activating p38 MAPK14. This intricate connection allows p38 alpha to execute precise regulation within cellular environments.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (2)
Recent publications for all applications. Explore the full list and refine your search
Nature communications 9:4685 PubMed30410062
2018
Applications
Unspecified application
Species
Unspecified reactive species
Scientific reports 8:16519 PubMed30410044
2018
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com