p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit is a single-wash 90-min Simplestep used to detect p38 MAPK alpha (pT180/Y182 + Total). The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Semi-quantitative Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 5 citations
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- Heliyon 9:e158052023The topoisomerase inhibitor CPT-11 prevents the growth and metastasis of lung cancer cells in nude mice by inhibiting EGFR/MAPK signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingqiu Yin,Junling Xie,Feng Peng,Liming Tan,Yun Xiao,Huiying Zheng,Lingzhi Yin,Huijing Situ,Shanqiang ZhangPubMed 37251857
- Mediators of inflammation 2022:11085692022Prevents Ulcerative Colitis by Promoting M2 Macrophage Polarization through Blocking the TLR4/MAPK/NF-B Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Sun,Ping Zhao,Xufeng Ding,Fang Li,Jie Jiang,Hua Huang,Lijiang JiPubMed 36619207
- Basic & clinical pharmacology & toxicology 132:154-1702022Berberine regulates pulmonary inflammatory microenvironment and decreases collagen deposition in response to bleomycin-induced pulmonary fibrosis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng Yang,Mengni Bian,Junbing Ma,Yonghe Dong,Dan Yang,Min Qiu,Zhixiang GaoPubMed 36433932
- Clinical proteomics 19:332022Proteomics-based evaluation of the mechanism underlying vascular injury via DNA interstrand crosslinks, glutathione perturbation, mitogen-activated protein kinase, and Wnt and ErbB signaling pathways induced by crotonaldehyde.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing-Zhang Xie,Jun-Li Liu,Qing-Zu Gao,De-Ying Bo,Lei Wang,Xiao-Chun Zhou,Meng-Meng Zhao,Yu-Chao Zhang,Yu-Jing Zhang,Guo-An Zhao,Lu-Yang JiaoPubMed 36002804
- Fundamental & clinical pharmacology 36:306-3232021Vitamin D3 potentiates the nephroprotective effects of vildagliptin-metformin combination in a rat model of metabolic syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNehal S Wahba,Rasha H Abdel-Ghany,Salah A Ghareib,Mohamed Abdel-Aal,Amira E Alsemeh,Dina SabryPubMed 34453360
- Journal of cellular biochemistry :2018Overexpression of the lncRNA FER1L4 inhibits paclitaxel tolerance of ovarian cancer cells via the regulation of the MAPK signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiwei Liu et. al.PubMed 30444026
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Homogenate, Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human, Mouse
- Assay time
- 1h 30m
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
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p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit is a single-wash 90-min Simplestep used to detect p38 MAPK alpha (pT180/Y182 + Total). The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Semi-quantitative Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 5 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Homogenate, Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human, Mouse
- Assay time
- 1h 30m
- Assay Platform
- Microplate
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample (pT180/Y182) | n 6 | C.V. 4.6 |
Sample Overall | n 6 | C.V. 9.2 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample (pT180/Y182) | n 3 | C.V. 2.5 |
Sample Overall | n 3 | C.V. 6.4 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit (ab176664) has been re-developed with new capture and detector antibodies. This new kit has the same name but a different product number (ab221013). We have identified new recombinant monoclonal antibodies to use in the SimpleStep ELISA platform that provide a higher sensitivity when quantifying Total p38 MAPKα protein in human and mouse cells.
Abcam's p38 MAPKα (pT180/Y182) and p38 MAPKα (Total) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the semi-quantitative measurement of p38 MAPKα (pT180/Y182) and Total p38 MAPKα protein in human and mouse cells.
The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Sensitivity:
p38 MAPK (pT180/Y182) = 0.2 ng/mL
p38 MAPK (Total) = 0.3 ng/mL
As of May 2020, this kit was reformulated with new antibodies to maintain continued long term supply.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
P38 alpha also known as MAPK14 is a significant member of the MAP kinase family involved in cellular response to stress signals. This protein has a molecular mass of about 38 kDa and is expressed in various tissues throughout the body. p38 alpha plays an important role in the signal transduction pathways that regulate inflammatory responses and cell differentiation. Its activity is modulated by multiple upstream kinases leading to cell-specific effects that are important for organismal homeostasis.
- Biological function summary
P38 alpha MAPK14 is a part of a larger mitogen-activated protein kinase (MAPK) complex where it serves to mediate signals from external stressors to the appropriate cellular processes. It is particularly active in its roles involving inflammation and apoptosis regulation. The protein interacts with other members of the MAPK family and additional proteins such as TAB1 to conduct these biological signals efficiently.
- Pathways
P38 alpha integrates into the p38 MAPK pathway and the NF-kB signaling pathway which are essential for managing cellular stress responses and inflammatory reactions. It closely interacts with other proteins like MKK3 and MKK6 which are directly upstream regulators phosphorylating and activating p38 MAPK14. This intricate connection allows p38 alpha to execute precise regulation within cellular environments.
- Associated diseases and disorders
P38 alpha MAPK14 is prominently associated with inflammatory diseases such as rheumatoid arthritis and cardiovascular disorders. In these conditions its aberrant activation or expression can lead to pathological inflammation and tissue damage. Additionally p38 alpha’s connection with TNF-alpha in inflammation highlights its relevance in therapeutic targets for related disorders reflecting the significance of its modulation to potentially mitigate disease progression.
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4 product images
ELISA - p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit (ab221013)
Example of p38 MAPKα (pT180/Y182) and p38 MAPKα (Total) cell lysate standard curve.
Raw data values are fitted to a four-parameter, variable slope curve (+/- SD).
ELISA - p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit (ab221013)
Example of p38 MAPKα (pT180/Y182) and p38 MAPKα (Total) recombinant protein standard curve.
Raw data values are fitted to a four-parameter, variable slope curve (+/- SD).
ELISA - p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit (ab221013)
Cell line analysis for Total p38 MAPKα from 100 µg/mL preparations of cell extracts.
Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).
ELISA - p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit (ab221013)
Induction of p38 MAPKα (pT180/Y182) phosphorylation in HeLa cells in response to anisomycin treatment.
HeLa cells were cultured in 96-well tissue culture plates and treated (15 min) with a dose-range of anisomycin before cell lysis. Data from quadruplicate measurements of p38 MAPKα (pT180/Y182) are plotted and compared against Total p38 MAPKα protein levels.
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