AB221013

p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit

SimpleStep
What is this?

(1 Review)

(6 Publications)

p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit is a single-wash 90-min Simplestep used to detect p38 MAPK alpha (pT180/Y182 + Total). The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Semi-quantitative Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 5 citations

4 Images

ELISA - p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit (AB221013)

ELISA

Supplier Data

ELISA - p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit (AB221013)

Example of p38 MAPKα (pT180/Y182) and p38 MAPKα (Total) recombinant protein standard curve.

Raw data values are fitted to a four-parameter, variable slope curve (+/- SD).

ELISA

Supplier Data

ELISA - p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit (AB221013)

Induction of p38 MAPKα (pT180/Y182) phosphorylation in HeLa cells in response to anisomycin treatment.

HeLa cells were cultured in 96-well tissue culture plates and treated (15 min) with a dose-range of anisomycin before cell lysis. Data from quadruplicate measurements of p38 MAPKα (pT180/Y182) are plotted and compared against Total p38 MAPKα protein levels.

ELISA

Supplier Data

ELISA - p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit (AB221013)

Example of p38 MAPKα (pT180/Y182) and p38 MAPKα (Total) cell lysate standard curve.

Raw data values are fitted to a four-parameter, variable slope curve (+/- SD).

ELISA

Supplier Data

ELISA - p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit (AB221013)

Cell line analysis for Total p38 MAPKα from 100 µg/mL preparations of cell extracts.

Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).

Key facts

Detection method

Colorimetric

Sample types

Tissue Homogenate, Cell Lysate

Reacts with

Human, Mouse

Results type

Semi-Quantitative

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit (ab176664) has been re-developed with new capture and detector antibodies. This new kit has the same name but a different product number (ab221013). We have identified new recombinant monoclonal antibodies to use in the SimpleStep ELISA platform that provide a higher sensitivity when quantifying Total p38 MAPKα protein in human and mouse cells.

Abcam's p38 MAPKα (pT180/Y182) and p38 MAPKα (Total) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the semi-quantitative measurement of p38 MAPKα (pT180/Y182) and Total p38 MAPKα protein in human and mouse cells.

The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

Sensitivity:

p38 MAPK (pT180/Y182) = 0.2 ng/mL

p38 MAPK (Total) = 0.3 ng/mL

As of May 2020, this kit was reformulated with new antibodies to maintain continued long term supply.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "(pT180/Y182)", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.4" }, { "reproducibilityType": "Intra", "sample": "(pT180/Y182)", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.2" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab221013-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"10X Wash Buffer PT", "number":"AB221013-CMP02", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"p38 MAPK alpha (Total) Capture Antibody", "number":"AB221013-CMP07", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"p38 MAPK alpha (pT180/Y182) Capture Antibody", "number":"AB221013-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB221013-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB221013-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB221013-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution", "number":"AB221013-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"5X Cell Extraction Buffer PTR", "number":"AB221013-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB221013-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized p38 MAPK alpha Control Lysate", "number":"AB221013-CMP04", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"p38 MAPK alpha (pT180/Y182) Detector Antibody", "number":"AB221013-CMP06", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"p38 MAPK alpha (Total) Detector Antibody", "number":"AB221013-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P38 alpha also known as MAPK14 is a significant member of the MAP kinase family involved in cellular response to stress signals. This protein has a molecular mass of about 38 kDa and is expressed in various tissues throughout the body. p38 alpha plays an important role in the signal transduction pathways that regulate inflammatory responses and cell differentiation. Its activity is modulated by multiple upstream kinases leading to cell-specific effects that are important for organismal homeostasis.

Biological function summary

P38 alpha MAPK14 is a part of a larger mitogen-activated protein kinase (MAPK) complex where it serves to mediate signals from external stressors to the appropriate cellular processes. It is particularly active in its roles involving inflammation and apoptosis regulation. The protein interacts with other members of the MAPK family and additional proteins such as TAB1 to conduct these biological signals efficiently.

Pathways

P38 alpha integrates into the p38 MAPK pathway and the NF-kB signaling pathway which are essential for managing cellular stress responses and inflammatory reactions. It closely interacts with other proteins like MKK3 and MKK6 which are directly upstream regulators phosphorylating and activating p38 MAPK14. This intricate connection allows p38 alpha to execute precise regulation within cellular environments.

P38 alpha MAPK14 is prominently associated with inflammatory diseases such as rheumatoid arthritis and cardiovascular disorders. In these conditions its aberrant activation or expression can lead to pathological inflammation and tissue damage. Additionally p38 alpha’s connection with TNF-alpha in inflammation highlights its relevance in therapeutic targets for related disorders reflecting the significance of its modulation to potentially mitigate disease progression.

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Product protocols

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Target data

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e15805 PubMed37251857

2023

The topoisomerase inhibitor CPT-11 prevents the growth and metastasis of lung cancer cells in nude mice by inhibiting EGFR/MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yingqiu Yin,Junling Xie,Feng Peng,Liming Tan,Yun Xiao,Huiying Zheng,Lingzhi Yin,Huijing Situ,Shanqiang Zhang

Mediators of inflammation 2022:1108569 PubMed36619207

2022

Prevents Ulcerative Colitis by Promoting M2 Macrophage Polarization through Blocking the TLR4/MAPK/NF-B Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Sun,Ping Zhao,Xufeng Ding,Fang Li,Jie Jiang,Hua Huang,Lijiang Ji

Basic & clinical pharmacology & toxicology 132:154-170 PubMed36433932

2022

Berberine regulates pulmonary inflammatory microenvironment and decreases collagen deposition in response to bleomycin-induced pulmonary fibrosis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Yang,Mengni Bian,Junbing Ma,Yonghe Dong,Dan Yang,Min Qiu,Zhixiang Gao

Clinical proteomics 19:33 PubMed36002804

2022

Proteomics-based evaluation of the mechanism underlying vascular injury via DNA interstrand crosslinks, glutathione perturbation, mitogen-activated protein kinase, and Wnt and ErbB signaling pathways induced by crotonaldehyde.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Zhang Xie,Jun-Li Liu,Qing-Zu Gao,De-Ying Bo,Lei Wang,Xiao-Chun Zhou,Meng-Meng Zhao,Yu-Chao Zhang,Yu-Jing Zhang,Guo-An Zhao,Lu-Yang Jiao

Fundamental & clinical pharmacology 36:306-323 PubMed34453360

2021

Vitamin D3 potentiates the nephroprotective effects of vildagliptin-metformin combination in a rat model of metabolic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Nehal S Wahba,Rasha H Abdel-Ghany,Salah A Ghareib,Mohamed Abdel-Aal,Amira E Alsemeh,Dina Sabry

Journal of cellular biochemistry : PubMed30444026

2018

Overexpression of the lncRNA FER1L4 inhibits paclitaxel tolerance of ovarian cancer cells via the regulation of the MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Siwei Liu,Bingyu Zou,Tian Tian,Xiaohui Luo,Banyun Mao,Xun Zhang,Huajiang Lei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit

ELISA - p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit (AB221013)

ELISA - p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit (AB221013)

ELISA - p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit (AB221013)

ELISA - p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit (AB221013)

Key facts

Detection method

Sample types

Reacts with

Results type

Assay time

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise