p70S6K (pT389) ELISA Kit is a single-wash 90-min Simplestep used to quantify p70S6K (pT389) with a sensitivity of 1 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
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- Disease models & mechanisms 16:2023Vemurafenib improves muscle histopathology in a mouse model of LAMA2-related congenital muscular dystrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAriany Oliveira-Santos,Marisela Dagda,Jennifer Wittmann,Robert Smalley,Dean J BurkinPubMed 37021539
- Medical oncology (Northwood, London, England) 39:1432022Unraveling the therapeutic potential of GANT61/Dactolisib combination as a novel prostate cancer modality.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohamed Youssef,Nermine Moussa,Maged W Helmy,Medhat HarounPubMed 35834137
- Molecular medicine reports 19:85-922018Downregulation of NLRP2 inhibits HUVEC viability by inhibiting the MAPK signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolu Zhang et. al.PubMed 30431084
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Homogenate, Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Mouse, Human
- Range
- 1 - 100 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression (PubMed:11500364, PubMed:12801526, PubMed:14673156, PubMed:15071500, PubMed:15341740, PubMed:16286006, PubMed:17052453, PubMed:17053147, PubMed:17936702, PubMed:18952604, PubMed:19085255, PubMed:19720745, PubMed:19935711, PubMed:19995915, PubMed:22017876, PubMed:23429703, PubMed:28178239). Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD (PubMed:11500364, PubMed:12801526, PubMed:14673156, PubMed:15071500, PubMed:15341740, PubMed:16286006, PubMed:17052453, PubMed:17053147, PubMed:17936702, PubMed:18952604, PubMed:19085255, PubMed:19720745, PubMed:19935711, PubMed:19995915, PubMed:22017876, PubMed:23429703, PubMed:28178239). Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex (PubMed:16286006). Upon mitogenic stimulation, phosphorylation by the mechanistic target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation (PubMed:16286006). The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B (PubMed:16286006). Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis (PubMed:17053147). Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR (PubMed:15341740). In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2 (PubMed:11500364). Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling (PubMed:19720745, PubMed:19935711, PubMed:19995915). Also involved in feedback regulation of mTORC1 and mTORC2 by phosphorylating DEPTOR (PubMed:22017876). Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function (By similarity). Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex (PubMed:17936702). The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function (PubMed:17936702). Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1 (PubMed:18952604). In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B (PubMed:17052453). May be involved in cytoskeletal rearrangement through binding to neurabin (By similarity). Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR (PubMed:23429703). Following activation by mTORC1, phosphorylates EPRS and thereby plays a key role in fatty acid uptake by adipocytes and also most probably in interferon-gamma-induced translation inhibition (PubMed:28178239).
Alternative names
STK14A, RPS6KB1, Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, 70 kDa ribosomal protein S6 kinase 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha, P70S6K1, p70-S6K 1, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA
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p70S6K (pT389) ELISA Kit is a single-wash 90-min Simplestep used to quantify p70S6K (pT389) with a sensitivity of 1 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Homogenate, Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Mouse, Human
- Range
- 1 - 100 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate
- Sensitivity
- = 1 ng/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample MCF7 Lysate | n 6 | C.V. 3.3 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample MCF7 Lysate | n 3 | C.V. 2.9 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's p70S6K (pT389) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the semi-quantitative measurement of p70S6K (pT389) protein in Human and mouse cells.
The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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5 product images
Sandwich ELISA - p70S6K (pT389) ELISA Kit (ab176651)
Typical cell lysate dilution series.
Example of a typical p70S6K (pT389) cell lysate dilution series. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - p70S6K (pT389) ELISA Kit (ab176651)
Typical recombinant protein standard curve.
Example of a typical p70S6K (pT389) recombinant protein standard curve. The proportion of total protein that is phosphorylated is unknown - data is indicative only. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - p70S6K (pT389) ELISA Kit (ab176651)
Linearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1 x Cell Extraction Buffer PTR. Data from duplicate measurements of p70S6K (pT389) are normalized and plotted.
Sandwich ELISA - p70S6K (pT389) ELISA Kit (ab176651)
Comparison of p70S6K (pT389) expression in different cell lines.
Cell line analysis for Total p70S6K from 100 μg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).
Sandwich ELISA - p70S6K (pT389) ELISA Kit (ab176651)
p70S6K (pT389) phosphorylation in response to insulin treatment.
Induction of p70S6K (pT389) phosphorylation in MCF-7 cells in response to insulin treatment. MCF-7 cells were cultured in 96-well tissue culture plates, serum-starved and treated (10 min) with a dose-range of insulin before cell lysis. Data from quadruplicate measurements of p70S6K (pT389) are plotted and compared against total p70S6K protein levels. Comparative p70S6K (pT389) and p70S6K (Total) data also shown by Western Blot.
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