p70S6K (pT389 + Total) ELISA Kit is a single-wash 90-min Simplestep used to detect p70S6K (pT389 + Total). The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Semi-quantitative Sandwich ELISA - 450 nm readout : works on any standard plate reader
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Homogenate, Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Mouse, Human
- Assay time
- 1h 30m
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
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p70S6K (pT389 + Total) ELISA Kit is a single-wash 90-min Simplestep used to detect p70S6K (pT389 + Total). The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Semi-quantitative Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Homogenate, Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Mouse, Human
- Assay time
- 1h 30m
- Assay Platform
- Microplate
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 6 | C.V. 4.5 |
Sample pT389 | n 6 | C.V. 3.3 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 3 | C.V. 4.1 |
Sample pT389 | n 3 | C.V. 2.9 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's p70S6K (pT389) and p70S6K (Total) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the semi-quantitative measurement of p70S6K (pT389) and Total p70S6K protein in Human and mouse cells.
The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
P70 S6 kinase also known as p70S6K or S6K1 is an important serine/threonine protein kinase with a molecular weight of about 70 kDa. This kinase plays a central role in cellular processes by phosphorylating the S6 ribosomal protein which stimulates protein synthesis. p70 S6 kinase sees expression in various tissues prominently in muscle and liver and also in other body tissues where cell growth and metabolism are actively regulated.
- Biological function summary
The function of p70 S6 kinase lies in the regulation of cell growth proliferation and survival. By modulating protein synthesis it impacts how cells respond to growth signals. p70S6K forms part of the larger TORC1 complex which includes mTOR and other associated proteins. This complex helps regulate both anabolic processes like protein and lipid synthesis and catabolic processes playing a prominent role in controlling the cell cycle. Its activity makes it essential for cellular energy homeostasis.
- Pathways
P70 S6 kinase is important to the PI3K/Akt/mTOR signaling pathway which is vital for cell growth and metabolism regulation. This pathway involves several interactions where p70S6K phosphorylates downstream targets and cooperates with proteins like mTOR Akt and eIF4E to promote protein synthesis. Additionally the pathway interfaces with the insulin signaling pathway signifying its role in nutrient sensing and growth regulation.
- Associated diseases and disorders
P70 S6 kinase exhibits a clear association with cancer and metabolic diseases. Its overactivation often contributes to cancer progression as the increased cell proliferation and survival promote tumor growth. p70S6K also entails connections with type 2 diabetes where its interaction with insulin signaling impacts glucose metabolism. The kinase associates with proteins like mTOR and PTEN whose mutations or dysregulation can cause these pathological conditions emphasizing its importance in disease dynamics.
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4 product images
ELISA - p70S6K (pT389 + Total) ELISA Kit (ab225582)
Example of p70S6K (pT389) and p70S6K (Total) cell lysate standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
ELISA - p70S6K (pT389 + Total) ELISA Kit (ab225582)
Example of p70S6K (pT389) recombinant protein standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
ELISA - p70S6K (pT389 + Total) ELISA Kit (ab225582)
Cell line analysis for Total p70S6K from 100 µg/mL preparations of cell extracts.
Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).
ELISA - p70S6K (pT389 + Total) ELISA Kit (ab225582)
Inhibition of p70S6K (pT389) phosphorylation in MCF-7 cells in response to wortmannin treatment.
MCF-7 cells were cultured in 96-well tissue culture plates, and treated (2 h) with a dose-range of wortmannin before stimulation with insulin (10 μg/mL for 30 min) and cell lysis. Data from triplicate measurements of p70S6K (pT389) are plotted and compared against total p70S6K protein levels.
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