JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314361

pan-HMR SYT1 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

pan-HMR SYT1 ELISA Kit is a single-wash 90-min Simplestep used to quantify pan-HMR SYT1 with a sensitivity of 7.218 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

SVP65, SYT, SYT1, Synaptotagmin-1, Synaptotagmin I, p65, SytI

3 Images
Sandwich ELISA - pan-HMR SYT1 ELISA Kit (AB314361)
  • sELISA

Supplier Data

Sandwich ELISA - pan-HMR SYT1 ELISA Kit (AB314361)

Interpolated concentration of native SYT1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human cortex extract 1 μg/mL, rat brain extract 0.4 μg/mL, and mouse brain extract 0.2 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - pan-HMR SYT1 ELISA Kit (AB314361)
  • sELISA

Supplier Data

Sandwich ELISA - pan-HMR SYT1 ELISA Kit (AB314361)

Example of SYT1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - pan-HMR SYT1 ELISA Kit (AB314361)
  • sELISA

Supplier Data

Sandwich ELISA - pan-HMR SYT1 ELISA Kit (AB314361)

Representative image of pan-HMR SYT1 ELISA Kit ELISA Kit (ab314361)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X pan-HMR SYT1 ELISA Kit ELISA Kit Capture Antibody

- 10X pan-HMR SYT1 Detector Antibody

- 10X pan-HMR SYT1 ELISA Kit ELISA Kit Lyophilised Purified Protein (2 vials)

- pan-HMR SYT1 ELISA Kit ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Human, Mouse, Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 7.218 pg/mL

Range

21.875 - 1400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

pan-HMR SYT1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SYT1 protein in Tissue extracts. Quantitate pan-HMR SYT1 with 7.218 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.36" } ]
style
left-column

Recovery

[ { "sample": "Tissue Extracts", "range": "110 - 121 %", "average": "= 117" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314361-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB314361-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB314361-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB314361-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB314361-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB314361-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB314361-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X pan-HMR SYT1 Capture Antibody", "number":"AB314361-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"pan-HMR SYT1 Lyophilized Recombinant Protein", "number":"AB314361-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X pan-HMR SYT1 Detector Antibody", "number":"AB314361-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB314361-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB314361-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB314361-CMP12", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Calcium sensor that participates in triggering neurotransmitter release at the synapse (By similarity). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (By similarity). It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes (PubMed : 23999003).
See full target information SYT1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Primary Antibodies

AB302627

Anti-SYT1 antibody [EPR24488-129]

20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT1 antibody [EPR24488-129] (AB302627)

  • 0
  • 0
Proteins & Peptides

AB108130

Recombinant Human SYT1 protein

SDS-PAGE - Recombinant Human SYT1 protein (AB108130)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com