pan-HMR VEGFC ELISA Kit is a single-wash 90-min Simplestep used to quantify pan-HMR VEGFC with a sensitivity of 4.375 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates KDR/VEGFR2 and FLT4/VEGFR3 receptors.
Vascular endothelial growth factor C, VEGF-C, Flt4 ligand, Vascular endothelial growth factor-related protein, Flt4-L, VRP, VEGFC
pan-HMR VEGFC ELISA Kit is a single-wash 90-min Simplestep used to quantify pan-HMR VEGFC with a sensitivity of 4.375 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
Sample | n | C.V. |
---|---|---|
Sample Supernatant | n 8 | C.V. 6.9 |
Sample | n | C.V. |
---|---|---|
Sample Supernatant | n 3 | C.V. 8.2 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 85 | Range 77 - 89 % |
Sample type Heparin Plasma | Average % = 85 | Range 79 - 94 % |
Sample type EDTA Plasma | Average % = 96 | Range 85 - 103 % |
Sample type Citrate plasma | Average % = 80 | Range 77 - 84 % |
Sample type Cell culture supernatant | Average % = 86 | Range 73 - 100 % |
pan-HMR VEGFC SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VEGFC protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant, Serum, Cit Plasma, EDTA Plasma, Serum. Quantitate pan-HMR VEGFC with 4.375 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
VEGFC also known as Vascular Endothelial Growth Factor C is a member of the VEGF family involved in angiogenesis and lymphangiogenesis. It has a molecular mass of approximately 35 kDa. VEGFC is expressed in various tissues with high levels in the heart lung and muscle. It acts primarily through its interaction with the VEGFR-3 receptor stimulating the formation of blood and lymphatic vessels. This protein plays an important role in the development and maintenance of the circulatory and lymphatic systems.
VEGFC contributes to the regulation of vascular and lymphatic endothelial cell functions. It is not known to be part of a larger protein complex but exerts its functions through interactions with receptors such as VEGFR-3. By promoting cell proliferation and migration VEGFC assists in the repair and regeneration of tissues. Its role in fluid balance is essential as it helps maintain tissue equilibrium and immune function.
VEGFC is integral to the VEGF signaling and lymphatic vessel development pathways. Within these processes VEGFC works alongside other proteins such as VEGFA and VEGFB to modulate vascular permeability and vessel formation. The interaction between VEGFC and the VEGFR-3 receptor specifically mediates lymphatic endothelial cell activation and function linking it to vital physiological processes like inflammation and tissue repair.
VEGFC is associated with cancer and lymphedema. Its role in promoting angiogenesis is critical in tumor growth and metastasis with its overexpression often linked to cancer progression. Furthermore insufficient VEGFC activity contributes to the development of lymphedema due to inadequate lymphatic drainage. In cancer pathology VEGFC works together with VEGFR-3 contributing to the abnormal growth of lymphatic vessels which in turn facilitates cancer cell dissemination.
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Example of VEGFC standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentration of native VEGFC was measured in duplicate at different sample concentrations. Undiluted samples are as follows: human serum 25%, human plasma (citrate) 25%, human plasma (EDTA) 25%, human plasma (heparin) 25%, and mouse plasma (citrate) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Interpolated concentrations of VEGFC in mouse serum 12.5%, mouse plasma (EDTA) 12.5%, rat serum 25%, rat plasma (citrate) 25%, rat plasma (EDTA) 25%, and rat plasma (heparin) 25% were below the detectable range.
Standard curve comparison between VEGFC SimpleStep ELISA kit (ab315050) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.
Interpolated concentration of native VEGFC was measured in duplicate at different sample concentrations. Undiluted samples are PC-3 cell supernatant 50%, HT-1080 cell supernatant 50%, and human urine 25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGFC concentration of detected samples was determined to be 53.4 pg/mL with a range of undetected – 88.8 pg/mL.
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