AB315050

pan-HMR VEGFC ELISA Kit

SimpleStep
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pan-HMR VEGFC ELISA Kit is a single-wash 90-min Simplestep used to quantify pan-HMR VEGFC with a sensitivity of 4.375 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders

View Alternative Names

Vascular endothelial growth factor C, VEGF-C, Flt4 ligand, Vascular endothelial growth factor-related protein, Flt4-L, VRP, VEGFC

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ELISA - pan-HMR VEGFC ELISA Kit (AB315050)

ELISA

Supplier Data

ELISA - pan-HMR VEGFC ELISA Kit (AB315050)

sELISA

Supplier Data

Sandwich ELISA - pan-HMR VEGFC ELISA Kit (AB315050)

Standard curve comparison between VEGFC SimpleStep ELISA kit (ab315050) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - pan-HMR VEGFC ELISA Kit (AB315050)

Example of VEGFC standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - pan-HMR VEGFC ELISA Kit (AB315050)

Interpolated concentration of native VEGFC was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human serum 25%, human plasma (citrate) 25%, human plasma (EDTA) 25%, human plasma (heparin) 25%, and mouse plasma (citrate) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Interpolated concentrations of VEGFC in mouse serum 12.5%, mouse plasma (EDTA) 12.5%, rat serum 25%, rat plasma (citrate) 25%, rat plasma (EDTA) 25%, and rat plasma (heparin) 25% were below the detectable range.

sELISA

Supplier Data

Sandwich ELISA - pan-HMR VEGFC ELISA Kit (AB315050)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGFC concentration of detected samples was determined to be 53.4 pg/mL with a range of undetected – 88.8 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - pan-HMR VEGFC ELISA Kit (AB315050)

Interpolated concentration of native VEGFC was measured in duplicate at different sample concentrations. Undiluted samples are PC-3 cell supernatant 50%, HT-1080 cell supernatant 50%, and human urine 25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, Heparin Plasma, EDTA Plasma, Citrate plasma, Cell culture supernatant

Reacts with

Rat, Human, Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 4.375 pg/mL

Range

5.859 - 375 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

pan-HMR VEGFC ELISA Kit ab315050 is a rapid single-wash 90-min Sandwich ELISA to measure pan-HMR VEGFC in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 4.375 pg/mL.

How the assay works

pan-HMR VEGFC SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

pan-HMR VEGFC ELISA Kit ab315050 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.9" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.2" } ]

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Recovery

[ { "sample": "Serum", "range": "77 - 89 %", "average": "= 85" }, { "sample": "Heparin Plasma", "range": "79 - 94 %", "average": "= 85" }, { "sample": "EDTA Plasma", "range": "85 - 103 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "77 - 84 %", "average": "= 80" }, { "sample": "Cell culture supernatant", "range": "73 - 100 %", "average": "= 86" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab315050-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB315050-CMP08" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315050-CMP05" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB315050-CMP07" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB315050-CMP06" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB315050-CMP04" }, { "size":"20 x 1 Vial", "name":"pan-HMR VEGFC Lyophilized Recombinant Protein", "number":"AB315050-CMP03" }, { "size":"10 x 600 µL", "name":"10X pan-HMR VEGFC Capture Antibody", "number":"AB315050-CMP01" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB315050-CMP10" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315050-CMP09" }, { "size":"10 x 600 µL", "name":"10X pan-HMR VEGFC Detector Antibody", "number":"AB315050-CMP02" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315050-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315050-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315050-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315050-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB315050-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"pan-HMR VEGFC Lyophilized Recombinant Protein", "number":"AB315050-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X pan-HMR VEGFC Capture Antibody", "number":"AB315050-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB315050-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB315050-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X pan-HMR VEGFC Detector Antibody", "number":"AB315050-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315050-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VEGFC also known as Vascular Endothelial Growth Factor C is a member of the VEGF family involved in angiogenesis and lymphangiogenesis. It has a molecular mass of approximately 35 kDa. VEGFC is expressed in various tissues with high levels in the heart lung and muscle. It acts primarily through its interaction with the VEGFR-3 receptor stimulating the formation of blood and lymphatic vessels. This protein plays an important role in the development and maintenance of the circulatory and lymphatic systems.

Biological function summary

VEGFC contributes to the regulation of vascular and lymphatic endothelial cell functions. It is not known to be part of a larger protein complex but exerts its functions through interactions with receptors such as VEGFR-3. By promoting cell proliferation and migration VEGFC assists in the repair and regeneration of tissues. Its role in fluid balance is essential as it helps maintain tissue equilibrium and immune function.

Pathways

VEGFC is integral to the VEGF signaling and lymphatic vessel development pathways. Within these processes VEGFC works alongside other proteins such as VEGFA and VEGFB to modulate vascular permeability and vessel formation. The interaction between VEGFC and the VEGFR-3 receptor specifically mediates lymphatic endothelial cell activation and function linking it to vital physiological processes like inflammation and tissue repair.

VEGFC is associated with cancer and lymphedema. Its role in promoting angiogenesis is critical in tumor growth and metastasis with its overexpression often linked to cancer progression. Furthermore insufficient VEGFC activity contributes to the development of lymphedema due to inadequate lymphatic drainage. In cancer pathology VEGFC works together with VEGFR-3 contributing to the abnormal growth of lymphatic vessels which in turn facilitates cancer cell dissemination.

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Product protocols

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Target data

Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates KDR/VEGFR2 and FLT4/VEGFR3 receptors.

See full target information VEGFC

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

pan-HMR VEGFC ELISA Kit

ELISA - pan-HMR VEGFC ELISA Kit (AB315050)

Sandwich ELISA - pan-HMR VEGFC ELISA Kit (AB315050)

Sandwich ELISA - pan-HMR VEGFC ELISA Kit (AB315050)

Sandwich ELISA - pan-HMR VEGFC ELISA Kit (AB315050)

Sandwich ELISA - pan-HMR VEGFC ELISA Kit (AB315050)

Sandwich ELISA - pan-HMR VEGFC ELISA Kit (AB315050)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Assay Kits

Secondary Antibodies

Primary Antibodies

Product promise