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AB178651

Anti-Parvovirus B 19 IgM ELISA Kit

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Anti-Parvovirus B 19 IgM ELISA Kit is an indirect ELISA for the qualitative detection of IgM class antibodies against Parvovirus B 19 in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Sensitivity

> 95 %

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Parvovirus B 19 IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Parvovirus B 19 in Human serum and plasma.

A 96-well plate has been precoated with Parvovirus B 19 antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Parvovirus B 19 antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Parvovirus B 19 IgM sample captured in plate.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 21, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 7, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab178651-1x96Tests", "assetComponentDetails": [ { "size":"1 x 2 mL", "name":"Parvovirus B 19 IgM Positive Control", "number":"AB178651-CMP09", "productcode":"" }, { "size":"1 x 3 mL", "name":"Parvovirus B 19 IgM Cut-off Control", "number":"AB178651-CMP05", "productcode":"" }, { "size":"1 x 2 mL", "name":"Parvovirus B 19 IgM Negative Control", "number":"AB178651-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover Foil", "number":"AB178651-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB178651-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"Parvovirus B 19 Anti-IgM HRP Conjugate", "number":"AB178651-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Parvovirus B 19 (IgM) Coated Microplate (12 x 8 wells)", "number":"AB178651-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB178651-CMP10", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgM Sample Diluent", "number":"AB178651-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB178651-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB178651-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Parvovirus B19 IgM typically called Parvovirus IgM represents the immunoglobulin M antibodies that the human body produces in response to Parvovirus B19 infection. This IgM has an important role during the early stages of infection and aids in identifying recent exposure to the virus. Parvovirus B19 is a small single-stranded DNA virus with a mass approximately 5-6 kDa. The expression of IgM antibodies occurs mostly within the bloodstream shortly after infection. Detection of these antibodies is commonly performed using diagnostic assays such as the Parvo ELISA test and Parvo IgM ELISA kit.
Biological function summary

The detection of Parvovirus B19 IgM indicates the body's activation of the immune response against Parvovirus B19. This response serves as the body's mechanism to eliminate the virus and prevent its replication. IgM is not part of a complex but plays an important stand-alone role in a host's immune defense. Its presence also aids in identifying other immune system activity linking to additional responses such as the production of immunoglobulin G (IgG) later in the infection phase.

Pathways

Parvovirus B19 IgM links to the immune response pathway particularly in the production and maturation of antibody responses. It interacts with other antibodies and immune complexes present during a virus infection aiding in directing B cell activation and eventual IgG formation. No direct protein-protein interaction occurs with Parvovirus B19 specific IgM within these pathways however its presence assists in shaping further immune activities critical for viral clearance.

Parvovirus B19 IgM serves as a significant marker for conditions like erythema infectiosum also known as fifth disease and transient aplastic crisis in individuals with hemolytic disorders. The presence of IgM antibodies indicates an active or recent infection. Parvovirus B19 plays an important role in these conditions with its viral proteins interacting indirectly with the host's blood cell production mechanisms potentially leading to complications in cases of anemia or compromised immune systems.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Parvovirus B 19 IgM
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

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Alternative Product
Assay Kits

AB178650

Anti-Parvovirus B 19 IgG ELISA Kit

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