PEGylated Protein (Polyethylene Glycol) ELISA Kit

Product details

PEGylated Protein (Polyethylene Glycol) ELISA Kit ab133065 is a competitive ELISA to measure PEGylated Protein (Polyethylene Glycol) in plasma, serum, tissue lysate samples and other biological samples with a sensitivity of 1 ng/mL.

How the assay works:

The kit detects both free and conjugated forms of PEG. Due to the nature of the antibody used, this assay is more sensitive to higher molecular weight PEG molecules.
A monoclonal antibody specific to PEGylated protein has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with a solution of PEGylated protein conjugated to biotin, followed by a solution of polyclonal antibody to PEGylated protein. The plate is washed to remove unbound reagents. A solution of streptavidin-HRP conjugate is then added. After further incubation the excess reagents are washed away and TMB substrate is added, which is catalyzed by HRP to generate a blue color. A stop solution changes this color from blue to yellow, and the intensity of this yellow coloration is inversely proportional to the amount of PEGylated protein captured in the plate.
This kit does not contain Tween 20.
Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. This is also the same case for other polyoxyethylene detergents. Therefore, it is recommended to avoid Tween 20 and other polyoxyethylene detergents when performing ELISA using anti-PEG products.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

PEGylated Protein (Polyethylene Glycol) ELISA Kit ab133065 protocol summary

1. Add samples and Biotinylated antigen to appropriate wells.
2. Incubate at room temperature
3. Wash and add prepared labeled Streptavidin HRP-conjugate to appropriate wells. Incubate at room temperature.
3. Wash and add TMB substrate to each well. Incubate at room temperature
4. Add Stop Solution to each well. Read immediately.

Poly(ethylene glycol) (PEG) is a widely used polymer in drug delivery systems and is often directly conjugated to a drug therapeutic. PEGylation of drug therapeutics is desirable as it has been found to increase the retention time, reduce the immunogenicity and increase stability towards metabolic enzymes. ab133065, the PEGylated protein ELISA Kit, is applicable for drug development and pharmaceutical manufacturing applications including drug formulations, pharmacokinetics analysis, drug comparison, lead candidate identification, lot release criteria and in-process QC studies. At this time, there are no long-term studies revealing the fate of this polymer in the body. The need for toxicology studies that would determine the accumulation of the metabolized PEGylated therapeutics in various tissues has been identified.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.