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AB237652

Pembrolizumab ELISA Kit

5

(1 Review)

|

(2 Publications)

Pembrolizumab ELISA Kit is a sandwich ELISA designed to quantify Pembrolizumab with a sensitivity of 30 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Can be used for pharmacokinetic (PK) studies
- Wide dynamic range - quantifies 30 - 1000 ng/mL
- For Research Use Only - Not intended for diagnostic use
1 Images
Sandwich ELISA - Pembrolizumab ELISA Kit (AB237652)
  • sELISA

Supplier Data

Sandwich ELISA - Pembrolizumab ELISA Kit (AB237652)

Example of Pembrolizumab standard curve.

Typical Standard Curve : This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Quantitative

Sensitivity

= 30 ng/mL

Range

30 - 1000 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Pembrolizumab ELISA Kit ab237652 is a sandwich ELISA to measure Pembrolizumab in serum, plasma with a sensitivity of 30 ng/mL.

How the assay works:

Note: Pembrolizumab ELISA kit ab237652 is intended for Research Use Only, not for use in diagnostic procedures.

This kit is a sandwich based ELISA kit. The color developed is proportional to the amount of Pembrolizumab in the sample or standard. Results of samples can be determined directly using the standard curve.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Pembrolizumab ELISA Kit ab237652 protocol summary

1. Add assay buffer to each well together with samples or standards or controls. Incubate
2. Wash
3. Add HRP-comjugate into each well. Incubate
4. Wash
5. Add TMB substrate
6. Add stop solution and read at 450 nm.

This product is manufactured by BioVision, an Abcam company and was previously called E4383 BioSim™ Pembrolizumab (Human) ELISA Kit. E4383-100 is the same size as the 96 test size of ab237652.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 30" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 30" } ]
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Recovery

[ { "sample": "Serum", "range": "85 - 115 %", "average": null } ]
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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab237652-96Test", "assetComponentDetails": [ { "size":"1 x 0.3 mL", "name":"Pembrolizumab Standard S5", "number":"AB237652-CMP09", "productcode":"" }, { "size":"2 x 50 mL", "name":"Assay Buffer", "number":"AB237652-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"HRP-conjugate Probe", "number":"AB237652-CMP02", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Pembrolizumab Standard S1", "number":"AB237652-CMP05", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Pembrolizumab Standard S2", "number":"AB237652-CMP06", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Pembrolizumab Standard S6", "number":"AB237652-CMP10", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Pembrolizumab Standard S7", "number":"AB237652-CMP11", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Pembrolizumab Standard S4", "number":"AB237652-CMP08", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Pembrolizumab Standard S3", "number":"AB237652-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Wash Buffer (10X)", "number":"AB237652-CMP12", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Plate sealers", "number":"AB237652-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB237652-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB substrate", "number":"AB237652-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB237652-CMP14", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Pembrolizumab
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:4698 PubMed38844770

2024

Ultrasound-mediated delivery of doxorubicin to the brain results in immune modulation and improved responses to PD-1 blockade in gliomas.

Applications

ELISA

Species

Human

Víctor A Arrieta,Andrew Gould,Kwang-Soo Kim,Karl J Habashy,Crismita Dmello,Gustavo I Vázquez-Cervantes,Irina Palacín-Aliana,Graysen McManus,Christina Amidei,Cristal Gomez,Silpol Dhiantravan,Li Chen,Daniel Y Zhang,Ruth Saganty,Meghan E Cholak,Surya Pandey,Matthew McCord,Kathleen McCortney,Brandyn Castro,Rachel Ward,Miguel Muzzio,Guillaume Bouchoux,Carole Desseaux,Michael Canney,Alexandre Carpentier,Bin Zhang,Jason M Miska,Maciej S Lesniak,Craig M Horbinski,Rimas V Lukas,Roger Stupp,Catalina Lee-Chang,Adam M Sonabend

Journal of clinical apheresis 35:227-230 PubMed32142176

2020

Lessons learned from immunoadsorption for hyperviscosity in IgM multiple myeloma-A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Philipp Gauckler,Johannes Leierer,Florian Kocher,Clemens Feistritzer,Wolfgang Willenbacher,Eberhard Gunsilius,Dominik Wolf,Hannes Neuwirt,Gert Mayer,Andreas Kronbichler
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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websiteProtocolBooklet
en
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Associated Products

Select an associated product type
Alternative Product
Assay Kits

AB237666

anti-Pembrolizumab ELISA Kit

elisa-kits

pembrolizumab-elisa-kit-ab237666

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(0 reviews)

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com