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AB133041

PGF2 alpha ELISA Kit

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(6 Publications)

PGF2 alpha ELISA Kit is a Competitive ELISA for the measurement of PGF2 alpha in Human, Pig in Biofluids samples.
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Competitive ELISA - PGF2 alpha ELISA Kit (AB133041)
  • cELISA

Supplier Data

Competitive ELISA - PGF2 alpha ELISA Kit (AB133041)

Representative Standard Curve using ab133041.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Plasma, Serum

Reacts with

Human, Pig

Assay type

Competitive

Sensitivity

= 6.71 pg/mL

Range

3.05 - 50000 pg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's PGF2 alpha in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of PGF2 alpha in Porcine Plasma, Porcine and Human Serum, Saliva, Urine and Tissue Culture Media.

A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-PGF2 alpha antigen and a polyclonal sheep antibody specific to PGF2 alpha. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of PGF2 alpha captured in the plate.

Prostaglandin F2α (PGF2α) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF2α is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF2α is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF2α is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF2α have also been associated with pre-eclampsia.

Cross Reactivity

Compound % Cross Reactivity
PGF2α 100
PGF1α 11.82
PGD2 3.62
6-keto-PGF1α 1.38
PGI2 1.25
PGE2 0.77
Thromboxane B2 0.77
8-iso PGF2α 0.73
PGE1 0.39
PGA2 <0.10
6,15-keto-13,14-dihydro-PGF1α <0.01
2-Arachidonoylglycerol <0.01
Anandamide <0.01
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Precision

[ { "reproducibilityType": "Inter", "sample": "High", "replicates": 0, "mean": "731 pg/mL", "standardDeviation": null, "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Inter", "sample": "Low", "replicates": 0, "mean": "47 pg/mL", "standardDeviation": null, "coefficientOfVariability": "9.7" }, { "reproducibilityType": "Inter", "sample": "Media", "replicates": 0, "mean": "290 pg/mL", "standardDeviation": null, "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Intra", "sample": "High", "replicates": 0, "mean": "916 pg/mL", "standardDeviation": null, "coefficientOfVariability": "4.9" }, { "reproducibilityType": "Intra", "sample": "Low", "replicates": 0, "mean": "83 pg/mL", "standardDeviation": null, "coefficientOfVariability": "13.1" }, { "reproducibilityType": "Intra", "sample": "Media", "replicates": 0, "mean": "405 pg/mL", "standardDeviation": null, "coefficientOfVariability": "6.8" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "= 100.3" }, { "sample": "Plasma", "range": null, "average": "= 96.9" }, { "sample": "Urine", "range": null, "average": "= 107" }, { "sample": "Saliva", "range": null, "average": "= 103.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab133041-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Sealer", "number":"AB133041-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB133041-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Donkey anti-sheep IgG Microplate (12 x 8 wells)", "number":"AB133041-CMP08", "productcode":"" }, { "size":"1 x 5 mL", "name":"Stop Solution", "number":"AB133041-CMP06", "productcode":"" }, { "size":"1 x 5 mL", "name":"Direct 8-iso-PGF2 alpha Antibody", "number":"AB133041-CMP07", "productcode":"" }, { "size":"1 x 5 mL", "name":"PGF2 alpha Alkaline Phosphatase Conjugate", "number":"AB133041-CMP02", "productcode":"" }, { "size":"1 x 0.5 mL", "name":"PGF2α Standard", "number":"AB133041-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"pNpp Substrate", "number":"AB133041-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Buffer", "number":"AB133041-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PGF2 alpha also known as prostaglandin F2α is a prostaglandin with a molecular weight of about 354.49 g/mol. It plays a role in various physiological processes. This compound expresses primarily in the uterus where it acts to induce uterine contractions. It also appears in the corpus luteum and other tissues such as the lungs and kidneys where it has different functions. Researchers frequently use PGF2 alpha ELISA as a method to measure its levels in biological fluids due to the precision and sensitivity of this assay.
Biological function summary

PGF2 alpha participates in reproductive and inflammatory responses. Its primary function involves luteolysis the process of corpus luteum regression necessary for the estrous cycle's progression. PGF2 alpha also promotes smooth muscle contraction including influencing bronchoconstriction and vasoconstriction. Furthermore it functions independently and does not require a complex to execute these actions although other prostaglandins may modulate its effects in specific contexts.

Pathways

PGF2 alpha sits within the prostaglandin signaling pathway which forms a part of the larger arachidonic acid cascade. It interacts with receptors like the FP receptor to mediate its physiological effects. PGF2 alpha also plays a role in the inflammatory pathway where it acts alongside prostaglandin E2 (PGE2) as part of the body's response to injury and infection. Through these signaling networks PGF2 alpha influences other proteins such as cyclooxygenase (COX) enzymes responsible for prostaglandin synthesis.

PGF2 alpha relates significantly to reproductive health issues and inflammatory conditions. For example its excessive activity can lead to dysmenorrhea characterized by painful menstrual cramps as it induces excessive uterine contractions. PGF2 alpha is also linked to asthma where its role in bronchoconstriction contributes to airway narrowing. In both cases the prostaglandin interacts with proteins like COX-2 which upregulates its production during inflammatory and reproductive processes.
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Product protocols

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Target data

See full target information PGF2 alpha
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 17: PubMed40004951

2025

Impact of Probiotic Supplementation and High-Intensity Interval Training on Primary Dysmenorrhea: A Double-Blind, Randomized Controlled Trial Investigating Inflammation and Hormonal Modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Yi Yang,Hao-Yu Chen,Chi-Hong Ho,Wen-Ching Huang

International journal of environmental research and public health 20: PubMed36767947

2023

Soluble ST2 as a New Oxidative Stress and Inflammation Marker in Metabolic Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Ignacio Roy,Eva Jover,Lara Matilla,Virginia Alvarez,Amaya Fernández-Celis,Maite Beunza,Elena Escribano,Alicia Gainza,Rafael Sádaba,Natalia López-Andrés

Endocrinology 164: PubMed36524678

2022

Decreased Expression of EZH2 in Granulosa Cells Contributes to Endometriosis-Associated Infertility by Targeting IL-1R2.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Lin,Xiaomei Tong,Yinli Zhang,Weijia Gu,Qianmeng Huang,Yi Zhang,Feng Zhuo,Fanxuan Zhao,Xiaoying Jin,Chao Li,Dong Huang,Songying Zhang,Yongdong Dai

The Journal of physiology 595:6623-6634 PubMed28877347

2017

Sex differences in the role of phospholipase A -dependent arachidonic acid pathway in the perivascular adipose tissue function in pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Abdulla A Ahmad,Michael D Randall,Richard E Roberts

Scientific reports 6:33515 PubMed27641436

2016

Physicochemistry and cardiovascular toxicity of metal fume PM2.5: a study of human coronary artery endothelial cells and welding workers.

Applications

Unspecified application

Species

Human

Chane-Yu Lai,Ching-Huang Lai,Hsiao-Chi Chuang,Chih-Hong Pan,Cheng-Chieh Yen,Wen-Yi Lin,Jen-Kun Chen,Lian-Yu Lin,Kai-Jen Chuang

International journal of molecular sciences 16:29980-95 PubMed26694364

2015

Dietary Compound Kaempferol Inhibits Airway Thickening Induced by Allergic Reaction in a Bovine Serum Albumin-Induced Model of Asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Daekeun Shin,Sin-Hye Park,Yean-Jung Choi,Yun-Ho Kim,Lucia Dwi Antika,Nurina Umy Habibah,Min-Kyung Kang,Young-Hee Kang
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