PGF2 alpha ELISA Kit is a Competitive ELISA kit for the measurement of PGF2 alpha in Human, Pig in Saliva, Urine, Plasma, Serum samples.
Images
Publications
Filter by
- International journal of environmental research and public health 20:2023Soluble ST2 as a New Oxidative Stress and Inflammation Marker in Metabolic Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIgnacio Roy,Eva Jover,Lara Matilla,Virginia Alvarez,Amaya Fernández-Celis,Maite Beunza,Elena Escribano,Alicia Gainza,Rafael Sádaba,Natalia López-AndrésPubMed 36767947
- Endocrinology 164:2022Decreased Expression of EZH2 in Granulosa Cells Contributes to Endometriosis-Associated Infertility by Targeting IL-1R2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Lin,Xiaomei Tong,Yinli Zhang,Weijia Gu,Qianmeng Huang,Yi Zhang,Feng Zhuo,Fanxuan Zhao,Xiaoying Jin,Chao Li,Dong Huang,Songying Zhang,Yongdong DaiPubMed 36524678
- The Journal of physiology 595:6623-66342017Sex differences in the role of phospholipase A -dependent arachidonic acid pathway in the perivascular adipose tissue function in pigs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbdulla A Ahmad,Michael D Randall,Richard E RobertsPubMed 28877347
- Scientific reports 6:335152016Physicochemistry and cardiovascular toxicity of metal fume PM2.5: a study of human coronary artery endothelial cells and welding workers.
- International journal of molecular sciences 16:29980-952015Dietary Compound Kaempferol Inhibits Airway Thickening Induced by Allergic Reaction in a Bovine Serum Albumin-Induced Model of Asthma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaekeun Shin et. al.PubMed 26694364
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Plasma, Serum
- Assay type
- Competitive
- Reactive species
- Human, Pig
- Range
- 3.05 - 50000 pg/mL
- Assay time
- 3h
- Sensitivity
- = 6.71 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Alternative names
Prostaglandin F2 alpha
What's included?
Recommended products
PGF2 alpha ELISA Kit is a Competitive ELISA kit for the measurement of PGF2 alpha in Human, Pig in Saliva, Urine, Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Plasma, Serum
- Assay type
- Competitive
- Reactive species
- Human, Pig
- Range
- 3.05 - 50000 pg/mL
- Assay time
- 3h
- Assay Platform
- Microplate
- Sensitivity
- = 6.71 pg/mL
Precision
Intra assay
| Sample | n | mean | C.V. |
|---|---|---|---|
Sample High | n 0 | mean 916 pg/mL | C.V. 4.9 |
Sample Low | n 0 | mean 83 pg/mL | C.V. 13.1 |
Sample Media | n 0 | mean 405 pg/mL | C.V. 6.8 |
Inter assay
| Sample | n | mean | C.V. |
|---|---|---|---|
Sample High | n 0 | mean 731 pg/mL | C.V. 3.1 |
Sample Low | n 0 | mean 47 pg/mL | C.V. 9.7 |
Sample Media | n 0 | mean 290 pg/mL | C.V. 5.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 100.3 | Range |
Sample type Plasma | Average % = 96.9 | Range |
Sample type Urine | Average % = 107 | Range |
Sample type Saliva | Average % = 103.5 | Range |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's PGF2 alpha in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of PGF2 alpha in Porcine Plasma, Porcine and Human Serum, Saliva, Urine and Tissue Culture Media.
A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-PGF2 alpha antigen and a polyclonal sheep antibody specific to PGF2 alpha. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of PGF2 alpha captured in the plate.
Prostaglandin F2α (PGF2α) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF2α is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF2α is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF2α is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF2α have also been associated with pre-eclampsia.
Cross Reactivity
| Compound | % Cross Reactivity |
| PGF2α | 100 |
| PGF1α | 11.82 |
| PGD2 | 3.62 |
| 6-keto-PGF1α | 1.38 |
| PGI2 | 1.25 |
| PGE2 | 0.77 |
| Thromboxane B2 | 0.77 |
| 8-iso PGF2α | 0.73 |
| PGE1 | 0.39 |
| PGA2 | <0.10 |
| 6,15-keto-13,14-dihydro-PGF1α | <0.01 |
| 2-Arachidonoylglycerol | <0.01 |
| Anandamide | <0.01 |
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PGF2 alpha also known as prostaglandin F2α is a prostaglandin with a molecular weight of about 354.49 g/mol. It plays a role in various physiological processes. This compound expresses primarily in the uterus where it acts to induce uterine contractions. It also appears in the corpus luteum and other tissues such as the lungs and kidneys where it has different functions. Researchers frequently use PGF2 alpha ELISA as a method to measure its levels in biological fluids due to the precision and sensitivity of this assay.
- Biological function summary
PGF2 alpha participates in reproductive and inflammatory responses. Its primary function involves luteolysis the process of corpus luteum regression necessary for the estrous cycle's progression. PGF2 alpha also promotes smooth muscle contraction including influencing bronchoconstriction and vasoconstriction. Furthermore it functions independently and does not require a complex to execute these actions although other prostaglandins may modulate its effects in specific contexts.
- Pathways
PGF2 alpha sits within the prostaglandin signaling pathway which forms a part of the larger arachidonic acid cascade. It interacts with receptors like the FP receptor to mediate its physiological effects. PGF2 alpha also plays a role in the inflammatory pathway where it acts alongside prostaglandin E2 (PGE2) as part of the body's response to injury and infection. Through these signaling networks PGF2 alpha influences other proteins such as cyclooxygenase (COX) enzymes responsible for prostaglandin synthesis.
- Associated diseases and disorders
PGF2 alpha relates significantly to reproductive health issues and inflammatory conditions. For example its excessive activity can lead to dysmenorrhea characterized by painful menstrual cramps as it induces excessive uterine contractions. PGF2 alpha is also linked to asthma where its role in bronchoconstriction contributes to airway narrowing. In both cases the prostaglandin interacts with proteins like COX-2 which upregulates its production during inflammatory and reproductive processes.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Competitive ELISA - PGF2 alpha ELISA Kit (ab133041)
Representative Standard Curve using ab133041.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com