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AB133056

PGF2 alpha High Sensitivity ELISA Kit

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(3 Publications)

PGF2 alpha High Sensitivity ELISA Kit is a Competitive ELISA for the measurement of PGF2 alpha High Sensitivity in in Biofluids, Cell Culture Media samples.

View Alternative Names

Prostaglandin F2 alpha

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Competitive ELISA - PGF2 alpha High Sensitivity ELISA Kit (AB133056)
  • cELISA

Supplier Data

Competitive ELISA - PGF2 alpha High Sensitivity ELISA Kit (AB133056)

Representative Standard Curve using ab133056.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum

Assay type

Competitive

Sensitivity

= 0.98 pg/mL

Range

1.95 - 2000 pg/mL

Assay time

2h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's PGF2α High Sensitivity in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of PGF2α in milk, culture supernatants, saliva, urine, serum and plasma.

A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-PGF2α antigen and a polyclonal sheep antibody specific to PGF2α. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of PGF2α captured in the plate.

Prostaglandin F2α (PGF2α) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF2α is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF2α is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF2α is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF2α have also been associated with preeclampsia.

Compound Cross Reactivity
PGF2α 100%
PGF1α 11.82%
PGD2 3.62%
6-keto-PGF1α 1.38%
PGI2 1.25%
PGE2 0.77%
Thromboxane B2 0.77%
8-iso PGF2α 0.73%
PGE1 0.39%
PGA2 <0.1%
6, 15-keto-13, 14-dihydro-PGF1α <0.01%
2-Arachidonoylglycerol <0.01%
Anandamide <0.01%
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Precision

[ { "reproducibilityType": "Inter", "sample": "10.1pmol/mL Spike", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.14" }, { "reproducibilityType": "Inter", "sample": "117.8pmol/mL Spike", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.97" }, { "reproducibilityType": "Inter", "sample": "35.81pmol/mL Spike", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.88" }, { "reproducibilityType": "Intra", "sample": "107.5pmol/mL Spike", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.53" }, { "reproducibilityType": "Intra", "sample": "11.5pmol/mL Spike", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.16" }, { "reproducibilityType": "Intra", "sample": "33.79pmol/mL Spike", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.94" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "= 100.3" }, { "sample": "Plasma", "range": null, "average": "= 96.9" }, { "sample": "Urine", "range": null, "average": "= 107" }, { "sample": "Saliva", "range": null, "average": "= 103.5" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PGF2 alpha also known as prostaglandin F2α is a prostaglandin with a molecular weight of about 354.49 g/mol. It plays a role in various physiological processes. This compound expresses primarily in the uterus where it acts to induce uterine contractions. It also appears in the corpus luteum and other tissues such as the lungs and kidneys where it has different functions. Researchers frequently use PGF2 alpha ELISA as a method to measure its levels in biological fluids due to the precision and sensitivity of this assay.
Biological function summary

PGF2 alpha participates in reproductive and inflammatory responses. Its primary function involves luteolysis the process of corpus luteum regression necessary for the estrous cycle's progression. PGF2 alpha also promotes smooth muscle contraction including influencing bronchoconstriction and vasoconstriction. Furthermore it functions independently and does not require a complex to execute these actions although other prostaglandins may modulate its effects in specific contexts.

Pathways

PGF2 alpha sits within the prostaglandin signaling pathway which forms a part of the larger arachidonic acid cascade. It interacts with receptors like the FP receptor to mediate its physiological effects. PGF2 alpha also plays a role in the inflammatory pathway where it acts alongside prostaglandin E2 (PGE2) as part of the body's response to injury and infection. Through these signaling networks PGF2 alpha influences other proteins such as cyclooxygenase (COX) enzymes responsible for prostaglandin synthesis.

PGF2 alpha relates significantly to reproductive health issues and inflammatory conditions. For example its excessive activity can lead to dysmenorrhea characterized by painful menstrual cramps as it induces excessive uterine contractions. PGF2 alpha is also linked to asthma where its role in bronchoconstriction contributes to airway narrowing. In both cases the prostaglandin interacts with proteins like COX-2 which upregulates its production during inflammatory and reproductive processes.
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Product protocols

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Target data

See full target information PGF2 alpha
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:13201-13224 PubMed39560493

2024

Prostaglandin E regulates senescence and post-senescence neoplastic escape in primary human keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Elise Srour,Nathalie Martin,Claire Drullion,Clémentine De Schutter,Joëlle Giroud,Adrien Pioger,Julie Deslé,Laure Saas,Joe Nassour,Julien Théry,Gauthier Decanter,Nicolas Penel,Chantal Vercamer,Clara Salazar-Cardozo,Corinne Abbadie,Olivier Pluquet

Communications biology 5:1169 PubMed36329259

2022

Prostaglandin-based rAAV-mediated glaucoma gene therapy in Brown Norway rats.

Applications

Unspecified application

Species

Unspecified reactive species

Kristina J Chern,Emily R Nettesheim,Christopher A Reid,Nathan W Li,Gavin J Marcoe,Daniel M Lipinski

Frontiers in immunology 9:456 PubMed29563916

2018

Old Yellow Enzyme from Exhibits Prostaglandin Fα Synthase Activity and Has a Key Role in Parasite Infection and Drug Susceptibility.

Applications

Unspecified application

Species

Unspecified reactive species

Florencia Díaz-Viraqué,María Laura Chiribao,Andrea Trochine,Fabiola González-Herrera,Christian Castillo,Ana Liempi,Ulrike Kemmerling,Juan Diego Maya,Carlos Robello
View all publications
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