Skip to main content

Phosph-RSK1 p90 (S380) ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phosph-RSK1 p90 (S380) in Human in Cell Lysate samples.

Be the first to review this product! Submit a review

Images

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Assay type

Semi-quantitative

Reactive species

Human

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB (By similarity). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration (PubMed:26158630).

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
2X Cell lysate buffer
1 x 5 mL
5X Assay Diluent
1 x 15 mL
HRP-conjugated anti-rabbit IgG concentrate (1000X)
1 x 25 µL
Pan-RSK1 p90 Coated Microplate
1 x 1 Unit
Positive Control - A431 cell lysate
1 x 1 Vial
Rabbit anti-phospho-Phosph-RSK1 p90 (S380)-antibody
2 x 1 Vial
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Phosph-RSK1 p90 (S380) ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phosph-RSK1 p90 (S380) in Human in Cell Lysate samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Assay type

Semi-quantitative

Reactive species

Human

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Phosph-RSK1 p90 (S380) ELISA Kit (ab279914) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated RSK1 p90 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-RSK1 p90. An anti-pan RSK1 p90 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and RSK1 p90 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-RSK1 p90 (S380) antibody is used to detect phosphorylated RSK1 p90. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RSK1 p90 (S380) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

Biological summary

RSK1 plays a fundamental role in cell proliferation and differentiation in the context of the p90 protein weight. It is an important component of the mitogen-activated protein kinase (MAPK) signaling pathway and forms part of multi-protein complexes that facilitate its activation and function. RSK1 influences various biological processes by phosphorylating transcription factors histone proteins and other kinases thereby regulating gene expression and protein synthesis. These actions highlight its influence in maintaining cellular homeostasis.

Mechanical summary

RSK1 also known as p90 ribosomal S6 kinase 1 or p90RSK is a serine/threonine kinase with an approximate mass of 90 kDa. It belongs to the family of proteins that integrate signals from various extracellular stimuli. This kinase is expressed in diverse tissues including brain lung liver and skeletal muscle. At the molecular level RSK1 phosphorylates various target proteins that are involved in regulating cellular growth and survival. Its activity is regulated by phosphorylation events that occur at specific sites such as Serine 238 (E238 in some literature) which are critical for its function.

Pathway

RSK1 p90 interacts significantly with the MAPK/ERK and PI3K/AKT pathways. It acts downstream of ERK where it receives activating signals that enable it to phosphorylate substrates within the cell. This role supports its interaction with proteins like Akt and mTOR which are critical mediators of cell growth signals. The interplay between these pathways enables cells to respond to growth factors and stress contributing to the regulation of development and metabolism.

Disease

RSK1 is implicated in the progression of cancer and neurological disorders. In cancer abnormal RSK1 signaling can lead to unchecked cell growth and survival where it often interfaces with proteins such as C-RAF and BCL-2 to promote oncogenic pathways. In neurological disorders RSK1 influences pathways that regulate neuron survival and plasticity connecting it to proteins like CREB. Understanding RSK1's role in these conditions can provide insight into targeted therapies that may mitigate these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Phosph-RSK1 p90 (S380) ELISA Kit (ab279914), expandable thumbnail

    Sandwich ELISA - Phosph-RSK1 p90 (S380) ELISA Kit (ab279914)

    Positive Control.

    A431 cells were treated with EGF at 37°C for 20 min.

    Cells were lysed in Cell Lysate Buffer (4 x 107 cells/ml).

    Serial dilutions of lysates were analyzed.

  • Sandwich ELISA - Phosph-RSK1 p90 (S380) ELISA Kit (ab279914), expandable thumbnail

    Sandwich ELISA - Phosph-RSK1 p90 (S380) ELISA Kit (ab279914)

    A431 cells treated with/without EGF. A431 cells were untreated or treated with 100 ng/ml EGF for 20 minutes.

  • Sandwich ELISA - Phosph-RSK1 p90 (S380) ELISA Kit (ab279914), expandable thumbnail

    Sandwich ELISA - Phosph-RSK1 p90 (S380) ELISA Kit (ab279914)

    A431 cells treated with/without EGF. A431 cells were untreated or treated with 100 ng/ml EGF for 20 minutes.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com