Phospho-ABL1 (Y245) and Total ABL1 ELISA Kit
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- sELISA
Supplier Data
Sandwich ELISA - Phospho-ABL1 (Y245) and Total ABL1 ELISA Kit (AB279728)
Western Blot analysis of Jurkat cells treated with/without Pervanadate.
Jurkat cells were treated with Pervanadate.
- sELISA
Supplier Data
Sandwich ELISA - Phospho-ABL1 (Y245) and Total ABL1 ELISA Kit (AB279728)
Jurkat cells treated with/without Pervanadate.
Jurkat cells were treated with Pervanadate. Cell lysates were analyzed using this phosphoELISA.
- sELISA
Supplier Data
Sandwich ELISA - Phospho-ABL1 (Y245) and Total ABL1 ELISA Kit (AB279728)
Positive Control.
Jurkat cells were treated with Pervanadate. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.
Reactivity data
Product details
Phospho-ABL1 (Y245) and Total ABL1 ELISA Kit (ab279728) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated ABL1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-ABL1 and total ABL1. An anti-pan ABL1 has been coated onto a 96-well plate. Samples are pipetted into the wells and ABL1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho ABL1 antibody is added to detect phosphorylated ABL1. In the remaining wells, mouse anti-pan-ABL1 antibody is used to detect pan ABL1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated anti-mouse IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ABL1 (Y245) or pan ABL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ABL1 functions by regulating key processes like cell cycle progression actin dynamics and cell adhesion. ABL1 participates as part of larger protein complexes that modulate cellular movement and gene transcription. When activated it phosphorylates a range of substrates that leads to various cellular outcomes. ABL1 operates in the cytoplasm and nucleus influencing both cytoskeletal rearrangement and DNA repair which highlights its critical function in maintaining cellular integrity and response to damage.
Pathways
ABL1 interacts in both the mitogenic and apoptotic pathways including involvement in the MAPK and PI3K/AKT pathways. ABL1 interfaces with proteins like CRK and GRB2 in these pathways integrating signals that determine cell fate decisions. Through its kinase activity ABL1 mediates signaling cascades that impact cellular growth and survival responding dynamically to internal and external cues.
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