Phospho-Acetyl Coenzyme A Carboxylase (S79) and Total Acetyl Coenzyme A Carboxylase ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-Acetyl Coenzyme A Carboxylase (S79) and Total Acetyl Coenzyme A Carboxylase in Human in Cell Lysate samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
What's included?
Recommended products
Phospho-Acetyl Coenzyme A Carboxylase (S79) and Total Acetyl Coenzyme A Carboxylase ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-Acetyl Coenzyme A Carboxylase (S79) and Total Acetyl Coenzyme A Carboxylase in Human in Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Phospho-Acetyl Coenzyme A Carboxylase (S79) and Total Acetyl Coenzyme A Carboxylase ELISA Kit (ab279730) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated ACC1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-ACC1 and Total ACC1. An anti-pan ACC1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and ACC1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho ACC1 antibody is added to detect phosphorylated ACC1. In the remaining wells, biotinylated anti-pan-ACC1 antibody is used to detect pan ACC1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ACC1 (S79) or pan ACC1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Acetyl Coenzyme A Carboxylase (ACC) also known as acetyl-CoA carboxylase or ACAC is an enzyme that plays an important role in fatty acid metabolism. Mechanically it catalyzes the biotin-dependent carboxylation of acetyl coenzyme A (acetyl-CoA) to produce malonyl-CoA which is an important precursor in the biosynthesis of fatty acids. The molecular weight of ACC is approximately 265 kDa. Humans express this enzyme in multiple tissues such as the liver adipose tissue and mammary glands.
- Biological function summary
Acetyl Coenzyme A Carboxylase contributes to fatty acid synthesis and regulation of metabolism. ACC exists in two main isoforms ACC1 which is found mainly in lipogenic tissues and ACC2 which is associated with oxidative tissues. These isoforms form part of larger complexes within the cell interacting with other enzymes and proteins to regulate metabolic processes. ACC also affects the synthesis of long-chain fatty acids by regulating the amount of malonyl-CoA available as a building block.
- Pathways
Acetyl Coenzyme A Carboxylase plays a role in the synthesis of fatty acids and their cellular metabolism. This enzyme is a component of the lipogenesis pathway where it transforms acetyl-CoA to malonyl-CoA a step critical for fatty acid elongation. ACC interacts with proteins such as fatty acid synthase to carry out its function within these metabolic pathways. Additionally malonyl-CoA produced by ACC serves as a regulator of carnitine palmitoyltransferase 1 integrating with the fatty acid oxidation pathway.
- Associated diseases and disorders
Alterations in the function of acetyl Coenzyme A Carboxylase link to conditions like obesity and type 2 diabetes. Overexpression of ACC can result in increased fat storage contributing to obesity while its inhibition has been considered a strategy to counter insulin resistance in diabetes. In cancer dysregulation of ACC especially ACC1 can lead to altered lipid synthesis promoting tumor growth. ACC1 interacts with other proteins such as AMP-activated protein kinase (AMPK) which senses energy status and is involved in the regulation of ACC activity thereby influencing these diseases.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sandwich ELISA - Phospho-Acetyl Coenzyme A Carboxylase (S79) and Total Acetyl Coenzyme A Carboxylase ELISA Kit (ab279730)
Positive Control.
HeLa cells were treated with Calyculin A. Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.
Sandwich ELISA - Phospho-Acetyl Coenzyme A Carboxylase (S79) and Total Acetyl Coenzyme A Carboxylase ELISA Kit (ab279730)
HeLa cells treated with/without Calyculin A.
HeLa cells were untreated or treated with Calyculin A. Cell lysates were analyzed using this phosphoELISA.
Sandwich ELISA - Phospho-Acetyl Coenzyme A Carboxylase (S79) and Total Acetyl Coenzyme A Carboxylase ELISA Kit (ab279730)
Western Blot analysis of HeLa cells treated with/without Calyculin A.
HeLa cells were untreated or treated with Calyculin A. Cell lysates were analyzed using this western blot.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com