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AB279729

Phospho-Acetyl Coenzyme A Carboxylase (S79) ELISA Kit

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Phospho-Acetyl Coenzyme A Carboxylase (S79) ELISA Kit is a ELISA for the measurement of Phospho-Acetyl Coenzyme A Carboxylase (S79) in Human in Cell/Tissue Extracts samples.

View Alternative Names

ACC2, ACCB, ACACB, Acetyl-CoA carboxylase 2, ACC-beta

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Sandwich ELISA - Phospho-Acetyl Coenzyme A Carboxylase (S79) ELISA Kit (AB279729)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-Acetyl Coenzyme A Carboxylase (S79) ELISA Kit (AB279729)

HeLa cells treated with/without Calyculin A.

HeLa cells were untreated or treated with Calyculin A.

Sandwich ELISA - Phospho-Acetyl Coenzyme A Carboxylase (S79) ELISA Kit (AB279729)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-Acetyl Coenzyme A Carboxylase (S79) ELISA Kit (AB279729)

Western Blot analysis of HeLa cells treated with/without Calyculin A.

HeLa cells were untreated or treated with Calyculin A.

Sandwich ELISA - Phospho-Acetyl Coenzyme A Carboxylase (S79) ELISA Kit (AB279729)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-Acetyl Coenzyme A Carboxylase (S79) ELISA Kit (AB279729)

Positive Control.

HeLa cells were treated with Calyculin A. Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

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Primary Antibodies

AB22759

Anti-Fatty Acid Synthase antibody

KO Validated

Western blot - Anti-Fatty Acid Synthase antibody (AB22759)

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Reactivity data

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Product details

Phospho-Acetyl Coenzyme A Carboxylase (S79) ELISA Kit (ab279729) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Acetyl Coenzyme A Carboxylase protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Acetyl Coenzyme A Carboxylase. An anti-pan Acetyl Coenzyme A Carboxylase antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Acetyl Coenzyme A Carboxylase present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-Acetyl Coenzyme A Carboxylase (S79) antibody is used to detect phosphorylated Acetyl Coenzyme A Carboxylase. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Acetyl Coenzyme A Carboxylase (S79) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279729-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279729-CMP08", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279729-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279729-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279729-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-ACC1 Coated Microplate", "number":"AB279729-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - treated HeLa cell lysate", "number":"AB279729-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-Acetyl Coenzyme A Carboxylase (S79)-antibody", "number":"AB279729-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279729-CMP04", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (1000X)", "number":"AB279729-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acetyl Coenzyme A Carboxylase (ACC) also known as acetyl-CoA carboxylase or ACAC is an enzyme that plays an important role in fatty acid metabolism. Mechanically it catalyzes the biotin-dependent carboxylation of acetyl coenzyme A (acetyl-CoA) to produce malonyl-CoA which is an important precursor in the biosynthesis of fatty acids. The molecular weight of ACC is approximately 265 kDa. Humans express this enzyme in multiple tissues such as the liver adipose tissue and mammary glands.
Biological function summary

Acetyl Coenzyme A Carboxylase contributes to fatty acid synthesis and regulation of metabolism. ACC exists in two main isoforms ACC1 which is found mainly in lipogenic tissues and ACC2 which is associated with oxidative tissues. These isoforms form part of larger complexes within the cell interacting with other enzymes and proteins to regulate metabolic processes. ACC also affects the synthesis of long-chain fatty acids by regulating the amount of malonyl-CoA available as a building block.

Pathways

Acetyl Coenzyme A Carboxylase plays a role in the synthesis of fatty acids and their cellular metabolism. This enzyme is a component of the lipogenesis pathway where it transforms acetyl-CoA to malonyl-CoA a step critical for fatty acid elongation. ACC interacts with proteins such as fatty acid synthase to carry out its function within these metabolic pathways. Additionally malonyl-CoA produced by ACC serves as a regulator of carnitine palmitoyltransferase 1 integrating with the fatty acid oxidation pathway.

Alterations in the function of acetyl Coenzyme A Carboxylase link to conditions like obesity and type 2 diabetes. Overexpression of ACC can result in increased fat storage contributing to obesity while its inhibition has been considered a strategy to counter insulin resistance in diabetes. In cancer dysregulation of ACC especially ACC1 can lead to altered lipid synthesis promoting tumor growth. ACC1 interacts with other proteins such as AMP-activated protein kinase (AMPK) which senses energy status and is involved in the regulation of ACC activity thereby influencing these diseases.
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Product protocols

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Target data

Mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA and plays a central role in fatty acid metabolism (PubMed : 16854592, PubMed : 19236960, PubMed : 19900410, PubMed : 20457939, PubMed : 20952656, PubMed : 26976583). Catalyzes a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed : 19236960, PubMed : 20457939, PubMed : 20952656, PubMed : 26976583). Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, negatively regulates fatty acid oxidation (By similarity). Together with its cytosolic isozyme ACACA, which is involved in de novo fatty acid biosynthesis, promotes lipid storage (By similarity).
See full target information ACACB phospho S79
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websiteProtocolBooklet
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Product promise

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