Phospho-AMPK alpha 1 (S487) ELISA Kit

Phospho-AMPK alpha 1 (S487) ELISA Kit (ab279733) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated AMPK alpha 1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-AMPK alpha 1. An anti-pan AMPK alpha 1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and AMPK alpha 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-AMPK alpha 1 (S487) antibody is used to detect phosphorylated AMPK alpha 1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of AMPK alpha 1 (S487) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.