Skip to main content

Phospho-ATM (S1981) and Total ATM ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-ATM (S1981) and Total ATM in Human in Cell Lysate samples.

Be the first to review this product! Submit a review

Images

Sandwich ELISA - Phospho-ATM (S1981) and Total ATM ELISA Kit (AB279740), expandable thumbnail
  • Sandwich ELISA - Phospho-ATM (S1981) and Total ATM ELISA Kit (AB279740), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Assay type

Semi-quantitative

Reactive species

Human

Reactivity data

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
2X Cell lysate buffer
1 x 5 mL
300X Streptavidin-Conjugated HRP
1 x 200 µL
5X Assay Diluent
1 x 15 mL
Biotinylated anti-pan-ATM detection antibody
1 x 1 Vial
HRP-conjugated anti-rabbit IgG concentrate (500X)
1 x 25 µL
Pan-ATM Coated Microplate
1 x 1 Unit
Positive Control - T47D cell lysate
1 x 1 Vial
Rabbit anti-phospho-ATM (S1981)-antibody
1 x 1 Vial
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Phospho-ATM (S1981) and Total ATM ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-ATM (S1981) and Total ATM in Human in Cell Lysate samples.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Assay type

Semi-quantitative

Reactive species

Human

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Phospho-ATM (S1981) and Total ATM ELISA Kit (ab279740) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated ATM protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-ATM and total ATM. An anti-pan ATM antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and ATM present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho ATM (S1981) antibody is added to detect phosphorylated ATM (S1981). In the remaining wells, biotinylated anti-pan-ATM antibody is used to detect pan ATM. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ATM (S1981) or pan ATM bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ATM also known as Ataxia Telangiectasia Mutated is a protein kinase with a molecular weight of approximately 370 kDa. ATM protein primarily resides in the cell nucleus and functions as a critical regulator of the cell cycle. It plays a significant role in the detection of DNA damage and initiation of repair processes. As part of its mechanical functions ATM phosphorylates serine and threonine residues on various substrates most notably in response to double-strand breaks in DNA. This activity is important for maintaining genomic stability.

Biological function summary

ATM acts as a coordinator in cellular response to DNA damage highly interacting with multiple components of the DNA repair machinery. It forms a complex with proteins like NBS1 and MRN complex facilitating repair by recruiting and activating other proteins involved in homologous recombination and non-homologous end joining pathways. ATM also modulates p53 activity a primary response factor in cellular stress management linking ATM to control of cell cycle arrest and apoptosis. This positions ATM as an integral part of maintaining cellular integrity in face of genomic insult.

Pathways

ATM integrates neatly within the DNA damage response and cell cycle control pathways. ATM's operative relationship with the MRN complex and its role in the PI3K-related protein kinase family helps initiate appropriate repair processes upon DNA damage detection. Additionally ATM regulates the activity of proteins such as Chk2 which further propagates signals to p53 influencing decisions between cell cycle arrest and apoptosis. These interactions link ATM closely to essential processes like DNA repair and cell survival highlighting its role in genomic maintenance.

Associated diseases and disorders

ATM mutations or dysregulation leads to Ataxia Telangiectasia an autosomal recessive disorder characterized by neurodegeneration immune deficiencies and cancer predisposition. ATM dysfunction also connects to cancer development particularly breast cancer where it transmits signals involving BRCA1 contributing to DNA repair through homologous recombination. Understanding ATM dynamics and related pathways has important implications for developing therapeutic strategies to manage or mitigate effects associated with its dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Phospho-ATM (S1981) and Total ATM ELISA Kit (ab279740), expandable thumbnail

    Sandwich ELISA - Phospho-ATM (S1981) and Total ATM ELISA Kit (ab279740)

    Positive Control.

    T47D cells were treated with UV.

    Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.

    Serial dilutions of lysates were analyzed in this ELISA.

  • Sandwich ELISA - Phospho-ATM (S1981) and Total ATM ELISA Kit (ab279740), expandable thumbnail

    Sandwich ELISA - Phospho-ATM (S1981) and Total ATM ELISA Kit (ab279740)

    UV treated T47D cells treated with/without LPP.

    T47D cells were treated with UV.

    Cell lysates were untreated or treated with Lambda Protein Phosphatase (LPP) and analyzed using this phosphoELISA and Western Blot.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com