JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB279740

Phospho-ATM (S1981) and Total ATM ELISA Kit

Be the first to review this product! Submit a review

|

(1 Publication)

Phospho-ATM (S1981) and Total ATM ELISA Kit is a ELISA for the measurement of Phospho-ATM (S1981) and Total ATM in Human in Cell/Tissue Extracts samples.
2 Images
Sandwich ELISA - Phospho-ATM (S1981) and Total ATM ELISA Kit (AB279740)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-ATM (S1981) and Total ATM ELISA Kit (AB279740)

Positive Control.

T47D cells were treated with UV.

Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-ATM (S1981) and Total ATM ELISA Kit (AB279740)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-ATM (S1981) and Total ATM ELISA Kit (AB279740)

UV treated T47D cells treated with/without LPP.

T47D cells were treated with UV.

Cell lysates were untreated or treated with Lambda Protein Phosphatase (LPP) and analyzed using this phosphoELISA and Western Blot.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB279742

Phospho-ATR (T1989) and Total ATR ELISA Kit

Sandwich ELISA - Phospho-ATR (T1989) and Total ATR ELISA Kit (AB279742)

  • 0
  • 0
Primary Antibodies

AB288357

Alexa Fluor® 647 Anti-ATM antibody [SP224]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-ATM antibody [SP224] (AB288357)

  • 0
  • 0
Assay Kits

AB113852

TMRE-Mitochondrial Membrane Potential Assay Kit

Functional Studies - TMRE-Mitochondrial Membrane Potential Assay Kit (AB113852)

  • 5
  • 4
Assay Kits

AB279816

Phospho-gamma H2A.X (S139) ELISA Kit

Sandwich ELISA - Phospho-gamma H2A.X (S139) ELISA Kit (AB279816)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Phospho-ATM (S1981) and Total ATM ELISA Kit (ab279740) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated ATM protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-ATM and total ATM. An anti-pan ATM antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and ATM present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho ATM (S1981) antibody is added to detect phosphorylated ATM (S1981). In the remaining wells, biotinylated anti-pan-ATM antibody is used to detect pan ATM. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ATM (S1981) or pan ATM bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279740-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"300X Streptavidin-Conjugated HRP", "number":"AB279740-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279740-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rabbit anti-phospho-ATM (S1981)-antibody", "number":"AB279740-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-ATM Coated Microplate", "number":"AB279740-CMP04", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279740-CMP09", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (500X)", "number":"AB279740-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Biotinylated anti-pan-ATM detection antibody", "number":"AB279740-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279740-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - T47D cell lysate", "number":"AB279740-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279740-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279740-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATM also known as Ataxia Telangiectasia Mutated is a protein kinase with a molecular weight of approximately 370 kDa. ATM protein primarily resides in the cell nucleus and functions as a critical regulator of the cell cycle. It plays a significant role in the detection of DNA damage and initiation of repair processes. As part of its mechanical functions ATM phosphorylates serine and threonine residues on various substrates most notably in response to double-strand breaks in DNA. This activity is important for maintaining genomic stability.
Biological function summary

ATM acts as a coordinator in cellular response to DNA damage highly interacting with multiple components of the DNA repair machinery. It forms a complex with proteins like NBS1 and MRN complex facilitating repair by recruiting and activating other proteins involved in homologous recombination and non-homologous end joining pathways. ATM also modulates p53 activity a primary response factor in cellular stress management linking ATM to control of cell cycle arrest and apoptosis. This positions ATM as an integral part of maintaining cellular integrity in face of genomic insult.

Pathways

ATM integrates neatly within the DNA damage response and cell cycle control pathways. ATM's operative relationship with the MRN complex and its role in the PI3K-related protein kinase family helps initiate appropriate repair processes upon DNA damage detection. Additionally ATM regulates the activity of proteins such as Chk2 which further propagates signals to p53 influencing decisions between cell cycle arrest and apoptosis. These interactions link ATM closely to essential processes like DNA repair and cell survival highlighting its role in genomic maintenance.

ATM mutations or dysregulation leads to Ataxia Telangiectasia an autosomal recessive disorder characterized by neurodegeneration immune deficiencies and cancer predisposition. ATM dysfunction also connects to cancer development particularly breast cancer where it transmits signals involving BRCA1 contributing to DNA repair through homologous recombination. Understanding ATM dynamics and related pathways has important implications for developing therapeutic strategies to manage or mitigate effects associated with its dysfunction.
style
left-column

Product protocols

style
left-column

Target data

style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:8304 PubMed37221295

2023

Sting and p53 DNA repair pathways are compromised in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas J Nelson,Yunhui Xu
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com