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AB279739

Phospho-ATM (S1981) ELISA Kit

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Phospho-ATM (S1981) ELISA Kit is a ELISA for the measurement of Phospho-ATM (S1981) in Human in Cell/Tissue Extracts samples.

View Alternative Names

Serine-protein kinase ATM, Ataxia telangiectasia mutated, A-T mutated, ATM

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Sandwich ELISA - Phospho-ATM (S1981) ELISA Kit (AB279739)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-ATM (S1981) ELISA Kit (AB279739)

T47D cells were treated with UV. Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-ATM (S1981) ELISA Kit (AB279739)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-ATM (S1981) ELISA Kit (AB279739)

UV treated T47D cells treated with/without LPP.

T47D cells were treated with UV.

Cell lysates were untreated or treated with Lambda Protein Phosphatase (LPP) and analyzed using this phosphoELISA and Western Blot.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-ATM (S1981) ELISA Kit (ab279739) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated ATM protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-ATM. An anti-pan ATM antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and ATM present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and rabbit anti-phospho-ATM (S1981) antibody is used to detect phosphorylated ATM. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ATM (S1981) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279739-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279739-CMP07", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279739-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279739-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-ATM (S1981)-antibody", "number":"AB279739-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279739-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279739-CMP03", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (500X)", "number":"AB279739-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-ATM Coated Microplate", "number":"AB279739-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - T47D cell lysate", "number":"AB279739-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATM also known as Ataxia Telangiectasia Mutated is a protein kinase with a molecular weight of approximately 370 kDa. ATM protein primarily resides in the cell nucleus and functions as a critical regulator of the cell cycle. It plays a significant role in the detection of DNA damage and initiation of repair processes. As part of its mechanical functions ATM phosphorylates serine and threonine residues on various substrates most notably in response to double-strand breaks in DNA. This activity is important for maintaining genomic stability.
Biological function summary

ATM acts as a coordinator in cellular response to DNA damage highly interacting with multiple components of the DNA repair machinery. It forms a complex with proteins like NBS1 and MRN complex facilitating repair by recruiting and activating other proteins involved in homologous recombination and non-homologous end joining pathways. ATM also modulates p53 activity a primary response factor in cellular stress management linking ATM to control of cell cycle arrest and apoptosis. This positions ATM as an integral part of maintaining cellular integrity in face of genomic insult.

Pathways

ATM integrates neatly within the DNA damage response and cell cycle control pathways. ATM's operative relationship with the MRN complex and its role in the PI3K-related protein kinase family helps initiate appropriate repair processes upon DNA damage detection. Additionally ATM regulates the activity of proteins such as Chk2 which further propagates signals to p53 influencing decisions between cell cycle arrest and apoptosis. These interactions link ATM closely to essential processes like DNA repair and cell survival highlighting its role in genomic maintenance.

ATM mutations or dysregulation leads to Ataxia Telangiectasia an autosomal recessive disorder characterized by neurodegeneration immune deficiencies and cancer predisposition. ATM dysfunction also connects to cancer development particularly breast cancer where it transmits signals involving BRCA1 contributing to DNA repair through homologous recombination. Understanding ATM dynamics and related pathways has important implications for developing therapeutic strategies to manage or mitigate effects associated with its dysfunction.
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Product protocols

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Target data

Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor (PubMed : 10550055, PubMed : 10839545, PubMed : 10910365, PubMed : 12556884, PubMed : 14871926, PubMed : 15064416, PubMed : 15448695, PubMed : 15456891, PubMed : 15790808, PubMed : 15916964, PubMed : 17923702, PubMed : 21757780, PubMed : 24534091, PubMed : 35076389, PubMed : 9733514). Recognizes the substrate consensus sequence [ST]-Q (PubMed : 10550055, PubMed : 10839545, PubMed : 10910365, PubMed : 12556884, PubMed : 14871926, PubMed : 15448695, PubMed : 15456891, PubMed : 15916964, PubMed : 17923702, PubMed : 24534091, PubMed : 9733514). Phosphorylates 'Ser-139' of histone variant H2AX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism (By similarity). Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FBXW7, FANCD2, NFKBIA, BRCA1, CREBBP/CBP, RBBP8/CTIP, FBXO46, MRE11, nibrin (NBN), RAD50, RAD17, PELI1, TERF1, UFL1, RAD9, UBQLN4 and DCLRE1C (PubMed : 10550055, PubMed : 10766245, PubMed : 10802669, PubMed : 10839545, PubMed : 10910365, PubMed : 10973490, PubMed : 11375976, PubMed : 12086603, PubMed : 15456891, PubMed : 19965871, PubMed : 21757780, PubMed : 24534091, PubMed : 26240375, PubMed : 26774286, PubMed : 30171069, PubMed : 30612738, PubMed : 30886146, PubMed : 30952868, PubMed : 38128537, PubMed : 9733515, PubMed : 9843217). May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation (PubMed : 19965871). Phosphorylates ATF2 which stimulates its function in DNA damage response (PubMed : 15916964). Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks (PubMed : 29203878). Phosphorylates TTC5/STRAP at 'Ser-203' in the cytoplasm in response to DNA damage, which promotes TTC5/STRAP nuclear localization (PubMed : 15448695). Also involved in pexophagy by mediating phosphorylation of PEX5 : translocated to peroxisomes in response to reactive oxygen species (ROS), and catalyzes phosphorylation of PEX5, promoting PEX5 ubiquitination and induction of pexophagy (PubMed : 26344566).
See full target information ATM phospho S1981
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