Phospho-ATR (T1989) and Total ATR ELISA Kit
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- sELISA
Supplier Data
Sandwich ELISA - Phospho-ATR (T1989) and Total ATR ELISA Kit (AB279742)
UV treated T47D cells.
T47D cells were untreated or treated with UV.
Cell lysates were analyzed using this phosphoELISA and Western Blot.
- sELISA
Supplier Data
Sandwich ELISA - Phospho-ATR (T1989) and Total ATR ELISA Kit (AB279742)
Positive Control.
T47D cells were exposed to 50 J/m2 of UV light followed by a 4 hours recovery period.
Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.
Serial dilutions of lysates were analyzed in this ELISA.
Reactivity data
Product details
Phospho-ATR (T1989) and Total ATR ELISA Kit (ab279742) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated ATR protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-ATR and total ATR. An anti-pan ATR antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and ATR present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho-ATR (T1989) antibody is added to detect phosphorylated ATR. In the remaining wells, mouse anti-pan-ATR antibody is used to detect pan ATR. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated anti-mouse IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ATR (T1989) or pan ATR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary information
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Biological function summary
ATR plays an essential role in maintaining genomic stability. It is part of a larger protein complex that includes ATRIP (ATR-interacting protein) which helps in localizing ATR to sites of DNA damage. Once activated ATR phosphorylates various substrates including CHK1 a critical checkpoint kinase involved in cell cycle arrest during DNA repair processes. The ability of ATR to coordinate with these proteins helps cells manage DNA damage effectively and prevent genomic instability.
Pathways
ATR functions centrally in the DNA damage response and repair mechanisms particularly the ATR-Chk1 pathway. This pathway interacts closely with the ATM (Ataxia Telangiectasia Mutated) pathway which also responds to DNA damage but usually to double-strand breaks. ATR primarily acts in response to replication stress and its activation leads to the arrest of the cell cycle allowing DNA repair to occur. This cooperation between ATR and ATM highlights their complementary roles in safeguarding genomic integrity under stress.
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Publications (1)
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Scientific reports 13:8304 PubMed37221295
2023
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