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AB279744

Phospho-Axl (Y779) and Total Axl ELISA Kit

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Phospho-Axl (Y779) and Total Axl ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-Axl (Y779) and Total Axl in Human in Cell/Tissue Extracts samples.
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Sandwich ELISA - Phospho-Axl (Y779) and Total Axl ELISA Kit (AB279744)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-Axl (Y779) and Total Axl ELISA Kit (AB279744)

Positive Control.

HUVEC cells were treated with Pervanadate (PV).

Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-Axl (Y779) and Total Axl ELISA Kit (AB279744)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-Axl (Y779) and Total Axl ELISA Kit (AB279744)

PV treated HUVEC cells.

HUVEC cells were untreated or treated with PV.

Cell lysates were analyzed using this phosphoELISA and Western Blot.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-Axl (Y779) and Total Axl ELISA Kit (ab279744) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Axl protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Axl and total Axl. An anti-pan Axl antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Axl present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho-Axl (Y779) antibody is added to detect phosphorylated Axl. In the remaining wells, biotinylated anti-pan-Axl antibody is used to detect pan Axl. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Axl (Y779) or pan Axl bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279744-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279744-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279744-CMP09", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279744-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279744-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-Axl Coated Microplate", "number":"AB279744-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Biotinylated anti-pan-Axl detection antibody", "number":"AB279744-CMP04", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (500X)", "number":"AB279744-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279744-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X Streptavidin-Conjugated HRP", "number":"AB279744-CMP11", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - HUVEC cell lysate", "number":"AB279744-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rabbit anti-phospho-Axl (Y779)-antibody", "number":"AB279744-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Axl protein also known as AXL receptor tyrosine kinase plays a significant role in cell signaling. It has a molecular weight of approximately 98 kDa. Axl is a transmembrane receptor that is expressed in a variety of tissues including the immune system reproductive organs and the central nervous system. It is mainly recognized for transmitting signals from the extracellular matrix into the cytoplasm by binding with its ligand Gas6.
Biological function summary

Axl is important in mediating cell survival proliferation and migration. It functions as part of the TAM family of receptors which also includes Tyro3 and Mer. Axl often forms complexes with these receptors to facilitate efficient signaling. This interaction triggers phosphorylation events that activate downstream signaling proteins reinforcing its influence in cellular functions.

Pathways

Axl is deeply involved in the PI3K-Akt and MAPK signaling pathways which are central to cellular growth and survival. In these pathways Axl interacts closely with proteins like PI3K and Akt to regulate processes such as apoptosis and metabolism. Its interaction with these pathways places Axl as an important modulator of intracellular signaling cascades.

Axl has been implicated in cancer progression and immune response dysregulation. In many cancer types Axl overexpression links to tumor invasion metastasis and resistance to therapy. Moreover Axl’s role in autoimmune diseases becomes evident through its association with the Gas6 protein. Exploring these connections furthers understanding of how Axl's aberrant activity influences health and disease.
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Product protocols

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Target data

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websiteProtocolBooklet
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