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AB279751

Phospho-BTK (Y551) ELISA Kit

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Phospho-BTK (Y551) ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-BTK (Y551) in Human in Cell/Tissue Extracts samples.

View Alternative Names

AGMX1, ATK, BPK, BTK, Tyrosine-protein kinase BTK, Agammaglobulinemia tyrosine kinase, B-cell progenitor kinase, Bruton tyrosine kinase

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Sandwich ELISA - Phospho-BTK (Y551) ELISA Kit (AB279751)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-BTK (Y551) ELISA Kit (AB279751)

Positive Control.

THP-1 cells were treated with Pervanadate at 37°C for 30 min.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-BTK (Y551) ELISA Kit (AB279751)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-BTK (Y551) ELISA Kit (AB279751)

THP-1 cells untreated/treated with Pervanadate. THP-1 cells were untreated or treated with Pervanadate for 10 mins.

Sandwich ELISA - Phospho-BTK (Y551) ELISA Kit (AB279751)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-BTK (Y551) ELISA Kit (AB279751)

THP-1 cells untreated/treated with Pervanadate. THP-1 cells were untreated or treated with Pervanadate for 10 mins.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-BTK (Y551) ELISA Kit (ab279751) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated BTK protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-BTK. An anti-pan BTK antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and BTK present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-BTK (Y551) antibody is used to detect phosphorylated BTK. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BTK (Y551) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279751-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279751-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-BTK Coated Microplate", "number":"AB279751-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279751-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - THP-1 cell lysate", "number":"AB279751-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279751-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279751-CMP08", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (1000X)", "number":"AB279751-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-BTK (Y551)-antibody", "number":"AB279751-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279751-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BTK or Bruton's tyrosine kinase is an important enzyme in the body that functions mainly in signaling pathways within cells. It is known for its involvement in the development and activation of B cells and is expressed in cells of the hematopoietic system. The BTK protein has a molecular weight of about 76 kDa. Researchers can study BTK using tools like the BTK ELISA kit which helps measure its presence in biological samples. Furthermore there are specific anti-BTK antibodies which assist in the detection of BTK bands during analysis. BTK inhibitors like BTK C481S have been developed to regulate its activity.
Biological function summary

BTK plays an important role in the immune response by transmitting signals from the B cell receptor to the inside of the cell which promotes B cell maturation and survival. It operates as part of a larger signal transduction complex that includes other proteins and molecules. For accurate quantification of BTK expression a BTK sandwich ELISA kit may be utilized capturing and revealing the BTK protein's presence in sample preparations.

Pathways

BTK associates with both the B cell receptor signaling and the PI3K-Akt pathway. These pathways are critical for the proper functioning and proliferation of B cells. BTK interacts with proteins like PLCγ2 and BLNK in the signaling cascade highlighting its central role in transmitting extracellular signals to elicit appropriate cellular responses.

BTK is closely linked to X-linked agammaglobulinemia (XLA) a genetic condition marked by an absence of mature B cells. This highlights the connection between BTK function and immune competence. Moreover aberrant BTK activity has been implicated in some types of B cell malignancies such as chronic lymphocytic leukemia (CLL). The BTK pathway's dysregulation in these disorders may involve interactions with proteins like SYK suggesting that targeting BTK could offer therapeutic benefits in managing such conditions.
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Product protocols

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Target data

Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling (PubMed : 19290921). Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation (PubMed : 19290921). After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members (PubMed : 11606584). PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK (PubMed : 11606584). BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways (PubMed : 16517732, PubMed : 17932028). Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway (PubMed : 16517732). The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense (PubMed : 16517732). Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells (PubMed : 16517732, PubMed : 17932028). Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation (PubMed : 16415872). BTK also plays a critical role in transcription regulation (PubMed : 19290921). Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes (PubMed : 19290921). BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B (PubMed : 19290921). Acts as an activator of NLRP3 inflammasome assembly by mediating phosphorylation of NLRP3 (PubMed : 34554188). Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR (PubMed : 9012831). GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression (PubMed : 9012831). ARID3A and NFAT are other transcriptional target of BTK (PubMed : 16738337). BTK is required for the formation of functional ARID3A DNA-binding complexes (PubMed : 16738337). There is however no evidence that BTK itself binds directly to DNA (PubMed : 16738337). BTK has a dual role in the regulation of apoptosis (PubMed : 9751072).
See full target information BTK phospho Y551
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