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AB279758

Phospho-c-Fos (T232) and Total c-Fos ELISA Kit

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Phospho-c-Fos (T232) and Total c-Fos ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-c-Fos (T232) and Total c-Fos in Human in Cell/Tissue Extracts samples.
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Sandwich ELISA - Phospho-c-Fos (T232) and Total c-Fos ELISA Kit (AB279758)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-c-Fos (T232) and Total c-Fos ELISA Kit (AB279758)

Positive Control.

HeLa cells were irradiated with Calyculin A.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-c-Fos (T232) and Total c-Fos ELISA Kit (AB279758)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-c-Fos (T232) and Total c-Fos ELISA Kit (AB279758)

HeLa cells treated with/without Calyculin A. HeLa cells were treated or untreated with Calyculin A.

Sandwich ELISA - Phospho-c-Fos (T232) and Total c-Fos ELISA Kit (AB279758)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-c-Fos (T232) and Total c-Fos ELISA Kit (AB279758)

HeLa cells treated with/without Calyculin A. HeLa cells were treated or untreated with Calyculin A.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-c-Fos (T232) and Total c-Fos ELISA Kit (ab279758) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated c-Fos protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-c-Fos and total c-Fos. An anti-pan c-Fos antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and c-Fos present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho-c-Fos (T232) antibody is added to detect phosphorylated c-Fos. In the remaining wells, mouse anti-pan-c-Fos antibody is used to detect pan c-Fos. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated anti-mouse IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of c-Fos (T232) or pan c-Fos bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "96Tests": { "sellingSize": "96 Tests", "publicAssetCode":"ab279758-96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279758-CMP10", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-mouse IgG concentrate (1000X)", "number":"AB279758-CMP02", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (500X)", "number":"AB279758-CMP12", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279758-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse anti-pan-c-Fos detection antibody", "number":"AB279758-CMP11", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - treated HeLa cell lysate", "number":"AB279758-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279758-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-c-Fos Coated Microplate", "number":"AB279758-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rabbit anti-phospho-c-Fos (T232)-antibody", "number":"AB279758-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279758-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279758-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The c-Fos protein also known as c-Fos is a component of the Fos family of transcription factors which includes FosB Fra-1 and Fra-2. These proteins play a role in regulating gene expression. c-Fos has a molecular weight of about 55 kDa. You usually find c-Fos expressed in various tissues including the brain providing a marker for neuronal activity and is often induced in response to stimuli like stress growth factors and mitogens. In research settings c-Fos staining and c-Fos immunohistochemistry are techniques commonly used to study activation patterns in cells and tissues.
Biological function summary

C-Fos acts as part of the Activator Protein-1 (AP-1) complex a group of DNA-binding proteins that regulate gene transcription. This complex forms when c-Fos and Jun proteins dimerize. The AP-1 complex controls several cellular processes such as proliferation differentiation and apoptosis. Due to its participation in these vital processes c-Fos influences cellular responses to external stimuli and can affect how cells behave under stress.

Pathways

C-Fos significantly contributes to the MAPK/ERK pathway which plays a role in cell growth and differentiation. It partners with Jun proteins to form functional transcription factors within the MAPK signaling cascade. Additionally c-Fos is involved in the JNK signaling pathway linking it to stress responses and apoptosis. These pathways highlight the partnerships between c-Fos and other proteins like ERK and JNK indicating their intertwined roles in cellular signaling networks.

Researchers associate c-Fos with cancer and neurodegenerative diseases. In cancer c-Fos expression correlates with changes in AP-1 activity which can lead to altered cell growth and proliferation potentially contributing to tumor formation. Its involvement in neurodegenerative diseases reveals its role in neuron apoptosis and cellular stress responses. In these scenarios proteins like Bcl-2 also play a part with CDK1 sharing connections in processes that affect cell cycle and apoptosis illustrating c-Fos's broad significance in both normal and pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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