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AB279844

Phospho-c-Jun (S63) and Total c-Jun ELISA Kit

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Phospho-c-Jun (S63) and Total c-Jun ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-c-Jun (S63) and Total c-Jun in Mouse in Cell/Tissue Extracts samples.
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Sandwich ELISA - Phospho-c-Jun (S63) and Total c-Jun ELISA Kit (AB279844)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-c-Jun (S63) and Total c-Jun ELISA Kit (AB279844)

Positive Control.

NIH/3T3 cells were treated with Anisomycin at 37°C for 30 mins.

Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-c-Jun (S63) and Total c-Jun ELISA Kit (AB279844)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-c-Jun (S63) and Total c-Jun ELISA Kit (AB279844)

NIH/3T3 cells treated with/without Anisomycin.

NIH/3T3 cells were untreated or treated with 10 μg/ml Anisomycin for 30 mins.

Sandwich ELISA - Phospho-c-Jun (S63) and Total c-Jun ELISA Kit (AB279844)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-c-Jun (S63) and Total c-Jun ELISA Kit (AB279844)

NIH/3T3 cells treated with/without Anisomycin.

NIH/3T3 cells were untreated or treated with 10 μg/ml Anisomycin for 30 mins.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Mouse

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-c-Jun (S63) and Total c-Jun ELISA Kit (ab279844) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in mouse cell lysates. By determining phosphorylated c-Jun protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of, mouse phospho-c-Jun and total c-Jun. An antipan c-Jun antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and c-Jun present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho-c-Jun (S63) antibody is added to detect phosphorylated c-Jun. In the remaining wells, rabbit anti-pan-c-Jun antibody is used to detect pan c-Jun. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of c-Jun (S63) or pan c-Jun bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein c-Jun is an important component of the AP-1 transcription factor complex and is often referred to by alternative names such as c-jun. It has a molecular weight of about 39 kDa. Scientists have found c-jun expressed in a wide range of tissues indicating its diverse roles in cellular functions. This protein plays a critical mechanical role by binding specific DNA sequences to regulate the transcription of various genes involved in cell proliferation and apoptosis.
Biological function summary

C-Jun impacts cellular activities by being a significant part of the AP-1 transcription factor complex interacting with other proteins such as Fos. This interaction allows c-Jun to modulate gene expression in response to cellular stimuli. In particular c-Jun influences cell cycle progression and differentiation. Therefore its activity is necessary for physiological and pathological processes. As c-jun's function remains tightly regulated any changes can have wide-ranging effects.

Pathways

C-Jun significantly influences the MAPK/ERK and JNK pathways. These pathways play important roles in cellular responses to stress growth factors and cytokines. Within these pathways c-Jun interacts with proteins such as JNK which phosphorylates c-Jun and enhances its activity. These interactions allow c-Jun to regulate target genes involved in important cellular processes without much delay. Understanding c-Jun's function in these pathways helps elucidate how cells control division survival and apoptosis.

C-Jun's dysregulation connects to various conditions including cancer and neurodegenerative diseases. Abnormal c-Jun activity can lead to oncogenesis by promoting unrestrained cellular proliferation. Additionally c-Jun involvement in disorders like Alzheimer's disease stems from its role in apoptotic pathways which may cause neuronal death. Accompanying proteins like JNK are also implicated in these conditions making the regulation and expression of c-Jun a focus for therapeutic research.
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Product protocols

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Target data

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websiteProtocolBooklet
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