AB279843

Phospho-c-Jun (S63) ELISA Kit

Name: Phospho-c-Jun (S63) ELISA Kit (ab279843) | Abcam
Brand: Abcam Limited
SKU: AB279843
Price: 1145 USD
Availability: InStock

Be the first to review this product! Submit a review

(0 Publication)

Phospho-c-Jun (S63) ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-c-Jun (S63) in Mouse in Cell/Tissue Extracts samples.

View Alternative Names

Transcription factor Jun, AH119, Activator protein 1, Proto-oncogene c-Jun, Transcription factor AP-1 subunit Jun, V-jun avian sarcoma virus 17 oncogene homolog, AP1, Jun A, Jun

3 Images

Sandwich ELISA - Phospho-c-Jun (S63) ELISA Kit (AB279843)

sELISA

Supplier Data

Sandwich ELISA - Phospho-c-Jun (S63) ELISA Kit (AB279843)

NIH/3T3 cells treated with/without Anisomycin.

NIH/3T3 cells were untreated or treated with 10 μg/ml Anisomycin for 30 mins.

sELISA

Supplier Data

Sandwich ELISA - Phospho-c-Jun (S63) ELISA Kit (AB279843)

Positive Control.

NIH/3T3 cells were treated with Anisomycin at 37°C for 30 mins.

Solubilize cells at 4 x 10⁷ cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

sELISA

Supplier Data

Sandwich ELISA - Phospho-c-Jun (S63) ELISA Kit (AB279843)

NIH/3T3 cells treated with/without Anisomycin.

NIH/3T3 cells were untreated or treated with 10 μg/ml Anisomycin for 30 mins.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Mouse

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Phospho-c-Jun (S63) ELISA Kit (ab279843) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in mouse cell lysates. By determining phosphorylated c-Jun protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of mouse phospho-c-Jun. An anti-pan c-Jun antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and c-Jun present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-c-Jun (S63) antibody is used to detect phosphorylated c-Jun. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of c-Jun (S63) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279843-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (1000X)", "number":"AB279843-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279843-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279843-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279843-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-c-Jun Coated Microplate", "number":"AB279843-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-c-Jun (S63)-antibody", "number":"AB279843-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - NIH/3T3 cell lysate", "number":"AB279843-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279843-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279843-CMP06", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein c-Jun is an important component of the AP-1 transcription factor complex and is often referred to by alternative names such as c-jun. It has a molecular weight of about 39 kDa. Scientists have found c-jun expressed in a wide range of tissues indicating its diverse roles in cellular functions. This protein plays a critical mechanical role by binding specific DNA sequences to regulate the transcription of various genes involved in cell proliferation and apoptosis.

Biological function summary

C-Jun impacts cellular activities by being a significant part of the AP-1 transcription factor complex interacting with other proteins such as Fos. This interaction allows c-Jun to modulate gene expression in response to cellular stimuli. In particular c-Jun influences cell cycle progression and differentiation. Therefore its activity is necessary for physiological and pathological processes. As c-jun's function remains tightly regulated any changes can have wide-ranging effects.

Pathways

C-Jun significantly influences the MAPK/ERK and JNK pathways. These pathways play important roles in cellular responses to stress growth factors and cytokines. Within these pathways c-Jun interacts with proteins such as JNK which phosphorylates c-Jun and enhances its activity. These interactions allow c-Jun to regulate target genes involved in important cellular processes without much delay. Understanding c-Jun's function in these pathways helps elucidate how cells control division survival and apoptosis.

C-Jun's dysregulation connects to various conditions including cancer and neurodegenerative diseases. Abnormal c-Jun activity can lead to oncogenesis by promoting unrestrained cellular proliferation. Additionally c-Jun involvement in disorders like Alzheimer's disease stems from its role in apoptotic pathways which may cause neuronal death. Accompanying proteins like JNK are also implicated in these conditions making the regulation and expression of c-Jun a focus for therapeutic research.

style

left-column

Product protocols

style

left-column

Target data

Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3' (PubMed : 14707112). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription factor complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity (PubMed : 2498083). Together with FOSB, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway (By similarity). Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation (PubMed : 17210646). Involved in activated KRAS-mediated transcriptional activation of USP28 (By similarity). Binds to the USP28 promoter (By similarity).

See full target information Transcription factor AP-1 phospho S63

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Phospho-c-Jun (S63) ELISA Kit

Sandwich ELISA - Phospho-c-Jun (S63) ELISA Kit (AB279843)

Sandwich ELISA - Phospho-c-Jun (S63) ELISA Kit (AB279843)

Sandwich ELISA - Phospho-c-Jun (S63) ELISA Kit (AB279843)

Key facts

Detection method

Sample types

Reacts with

Assay type

Assay Platform

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Assay Kits

Primary Antibodies

Assay Kits

Product promise